TY - JOUR AB - Corrigendum We were alerted by a post on Pubpeer that the image panel in Fig. 6A (transwell migration assay) contains several images that overlap with each other, even though they should represent independent experiments. We deeply regret this misrepresentation of results in Fig. 6A. Upon inspection of archived raw data from 2008, when the experiments were conducted, we found that our archived images from this experiment are incomplete, and regretfully, we were not able to reconstruct how or why the faulty figure assembly had occurred. Given the resulting lack of fullest confidence in the results shown in Fig. 6A, and by extension also in the associated quantification in Fig. 6B, we want to notify the readers that we wish to remove as a correction Fig. 6 and the corresponding paragraph in the Results section from the publication. The removal of Fig. 6 has in our judgement no effect on the validity of other results and the conclusions of this study. The transwell migration assay of Fig. 6 was independent of other experiments and was in support of expression (Fig. 1), binding (Fig. 2), in vivo mutant mouse (Figs. 3–5), explant culture (Fig. 7), and supplemental data studies, which all stand by themselves as valid. The authors would like to apologize for any inconvenience caused. AU - Maier, V. AU - Jolicoeur, C.* AU - Rayburn, H.* AU - Takegahara, N.* AU - Kumanogoh, A.* AU - Kikutani, H.* AU - Tessier-Lavigne, M.* AU - Wurst, W. AU - Friedel, R.H. C1 - 67555 C2 - 54067 CY - 525 B St, Ste 1900, San Diego, Ca 92101-4495 Usa TI - Corrigendum to Semaphorin 4C and 4G are ligands of Plexin-B2 required in cerebellar development [Mol. Cell. Neurosci., 2011 Feb;46(2):419-31]. JO - Mol. Cell. Neurosci. VL - 125 PB - Academic Press Inc Elsevier Science PY - 2023 SN - 1044-7431 ER - TY - JOUR AB - eural stem cells in the subventricular zone (SVZ) of the lateral ventricles give rise to new interneurons of the olfactory bulb (OB) throughout life. SVZ/OB neurogenesis is influenced by olfactory network activity, which modulates the survival of new neurons during their integration into the OB network. Previous work suggested that such activity-dependent survival is regulated via the CREB signalling pathway. Curiously, CREB signalling is already active during the early developmental stages of adult SVZ/OB neurogenesis. To investigate the role of cell autonomous CREB signalling during early stages of adult SVZ/OB neurogenesis, we ablated CREB-pathway activity in the SVZ/OB neurogenic lineage using a retroviral strategy. Surprisingly, loss of CREB signalling resulted in increased cell death and loss of expression of the neurogenic transcription factor Pax 6, and of a subset of neuronal proteins in migrating neurons of the RMS. Moreover, post-migratory neurons in the OB displayed impaired dendritic development. These results demonstrate an essential role for CREB signalling in maturation of newborn neurons in the OB and uncover a novel role for CREB signalling in the survival and maintenance of neuronal gene expression during the early stages of SVZ/OB neurogenesis. AU - Herold, S. AU - Jagasia, R. AU - Merz, K. AU - Wassmer, K. AU - Lie, D.C. C1 - 5954 C2 - 27655 SP - 79-88 TI - CREB signalling regulates early survival, neuronal gene expression and morphological development in adult subventricular zone neurogenesis. JO - Mol. Cell. Neurosci. VL - 46 IS - 1 PB - Elsevier PY - 2011 SN - 1044-7431 ER - TY - JOUR AB - Semaphorins and Plexins are cognate ligand-receptor families that regulate important steps during nervous system development. The Plexin-B2 receptor is critically involved in neural tube closure and cerebellar granule cell development, however, its specific ligands have only been suggested by in vitro studies. Here, we show by in vivo and in vitro analyses that the two Semaphorin-4 family members Sema4C and Sema4G are likely to be in vivo ligands of Plexin-B2. The Sema4C and Sema4G genes are expressed in the developing cerebellar cortex, and Sema4C and Sema4G proteins specifically bind to Plexin-B2 expressing cerebellar granule cells. To further elucidate their in vivo function, we have generated and analyzed Sema4C and Sema4G knockout mouse mutants. Like Plexin-B2-/- mutants, Sema4C-/- mutants reveal exencephaly and subsequent neonatal lethality with partial penetrance. Sema4C-/- mutants that bypass exencephaly are viable and fertile, but display distinctive defects of the cerebellar granule cell layer, including gaps in rostral lobules, fusions of caudal lobules, and ectopic granule cells in the molecular layer. In addition to neuronal defects, we observed in Sema4C-/- mutants also ventral skin pigmentation defects that are similar to those found in Plexin-B2-/- mutants. The Sema4G gene deletion causes no overt phenotype by itself, but combined deletion of Sema4C and Sema4G revealed an enhanced cerebellar phenotype. However, Sema4C/Sema4G double mutants showed overall less severe cerebellar phenotypes than Plexin-B2-/- mutants, indicating that further ligands of Plexin-B2 exist. In explant cultures of the developing cerebellar cortex, Sema4C promoted migration of cerebellar granule cell precursors in a Plexin-B2-dependent manner, supporting the model that a reduced migration rate of granule cell precursors is the basis for the cerebellar defects of Sema4C-/- and Sema4C/Sema4G mutants. AU - Maier, V. AU - Jolicoeur, C.* AU - Rayburn, H.* AU - Takegahara, N.* AU - Kumanogoh, A.* AU - Kikutani, H.* AU - Tessier-Lavigne, M.* AU - Wurst, W. AU - Friedel, R.H. C1 - 6048 C2 - 28771 CY - San Diego, CA SP - 419-431 TI - Semaphorin 4C and 4G are ligands of Plexin-B2 required in cerebellar development. JO - Mol. Cell. Neurosci. VL - 46 IS - 2 PB - Academic Press PY - 2011 SN - 1044-7431 ER - TY - JOUR AB - The Ca2+ stimulated adenylate cyclase 1 (AC1) is a key mediator of retinotopic map refinement and is required for the retraction response of retinal growth cones to the guidance cue ephrin-A5 We show here that AC1 is dynamically expressed in subpopulations of motor neurons in the spinal cord and sensory neurons of the dorsal root ganglia during development AC1 was first detected around E12 5 in motoneurons of the medial aspect of the lateral motor column (LMCm) and the lateral region of the medial motor column (MMCl) which project to the ventral limb and body wall musculature respectively Expression levels gradually increased until they reached a maximum at a time when peripheral sensory and motor axons branch and establish connections with their targets In barrelless mice where a mutation inactivates the AC1 gene sensory projections to the skin in the limbs and trunk region as well as innervations of the intercostal musculature provided by MMCl axons show increased branching These results suggest a function of AC1 in the formation of peripheral nerve trajectories such as branching and pruning after the initial projections have been laid down. AU - Haupt, C. AU - Langhoff, J. AU - Huber, A.B. C1 - 3841 C2 - 27997 SP - 439-448 TI - Adenylate Cyclase 1 modulates peripheral nerve branching patterns. JO - Mol. Cell. Neurosci. VL - 45 IS - 4 PB - Elsevier PY - 2010 SN - 1044-7431 ER - TY - JOUR AB - The orphan nuclear receptor NR4A2/Nurr1 is mandatory for the terminal differentiation of mesencephalic dopamine neurons in mammals, but a similar role has remained elusive in the homologous area of the fish brain, the posterior tuberculum. Using loss- and gain-of-function experiments in zebrafish, we show that NR4A2 is indeed responsible for the expression of tyrosine hydroxylase (TH) in selective subpopulations of dopamine cells in the posterior tuberculum, as well as in the pretectum, preoptic area and telencephalon. Cross sections of the neural tube reveal that cells expressing the proliferation marker PCNA, NR4A2 and TH are aligned along a mediolateral progression rather than overlapping populations, suggesting that NR4A2 does not simply regulate TH expression but also controls more general steps of progenitor commitment towards the fully differentiated DA neuronal state. Finally, in line with NR4A2+/- heterozygote mice, NR4A2 morphant fish are hyperactive. This behavioural phenotype is maintained throughout life, pointing to a developmental control of locomotor activity by NR4A2. Our results shed new light on NR4A2 function in the DA differentiation pathway, and stress the effect of DA dysregulation on the control of locomotor activity. AU - Blin, M.* AU - Norton, W.H.J. AU - Bally-Cuif, L. AU - Vernier, P.* C1 - 3650 C2 - 25857 SP - 592-604 TI - NR4A2 controls the differentiation of selective dopaminergic nuclei in the zebrafish brain. JO - Mol. Cell. Neurosci. VL - 39 IS - 4 PB - Elsevier PY - 2008 SN - 1044-7431 ER - TY - JOUR AB - Since the discovery of radial glia as the source of neurons, their heterogeneity in regard to neurogenesis has been described by clonal and time-lapse analysis in vitro. However, the molecular determinants specifying neurogenic radial glia differently from radial glia that mostly self-renew remain ill-defined. Here, we isolated two radial glial subsets that co-exist at mid-neurogenesis in the developing cerebral cortex and their immediate progeny. While one subset generates neurons directly, the other is largely non-neurogenic but also gives rise to Tbr2-positive basal precursors, thereby contributing indirectly to neurogenesis. Isolation of these distinct radial glia subtypes allowed determining interesting differences in their transcriptome. These transcriptomes were also strikingly different from the transcriptome of radial glia isolated at the end of neurogenesis. This analysis therefore identifies, for the first time, the lineage origin of basal progenitors and the molecular differences of this lineage in comparison to directly neurogenic and gliogenic radial glia. AU - Pinto, L. AU - Mader, M.T. AU - Irmler, M. AU - Gentilini, M.* AU - Santoni, F.* AU - Drechsel, D. AU - Blum, R.* AU - Stahl, R.* AU - Bulfone, A.* AU - Malatesta, P.* AU - Beckers, J. AU - Götz, M. C1 - 1820 C2 - 25434 SP - 15-42 TI - Prospective isolation of functionally distinct radial glial subtypes--lineage and transcriptome analysis. JO - Mol. Cell. Neurosci. VL - 38 IS - 1 PB - Elsevier PY - 2008 SN - 1044-7431 ER - TY - JOUR AB - Stress exposure can lead to the precipitation of psychiatric disorders in susceptible individuals, but the molecular underpinnings are incompletely understood. We used forced swimming in mice to reveal stress-regulated genes in the CA3 area of the hippocampus. To determine changes in the transcriptional profile 4 h and 8 h after stress exposure microarrays were used in the two mouse strains C57BL/6J and DBA/2J, which are known for their differential stress response. We discovered a surprisingly distinct set of regulated genes for each strain and followed selected ones by in situ hybridisation. Our results support the concept of a phased transcriptional reaction to stress. Moreover, we suggest novel stress-elicited pathways, which comprise a number of genes involved in the regulation of neuronal plasticity. Furthermore, we focused in particular on dihydropyrimidinase like 2, to which we provide evidence for its regulation by NeuroD, an important factor for neuronal activity-dependent dendritic morphogenesis. AU - Tsolakidou, A.* AU - Trümbach, D. AU - Panhuysen, M.* AU - Pütz, B.* AU - Deussing, J.M.* AU - Wurst, W. AU - Sillaber, I.* AU - Holsboer, F.* AU - Rein, T.* C1 - 1537 C2 - 25454 SP - 444-452 TI - Acute stress regulation of neuroplasticity genes in mouse hippocampus CA3 area - possible novel signalling pathways. JO - Mol. Cell. Neurosci. VL - 38 IS - 3 PB - Elsevier PY - 2008 SN - 1044-7431 ER - TY - JOUR AB - Appropriate neurogenesis and patterning of the forebrain requires the transcription factor Pax6, yet it is largely unknown how Pax6 exerts its effects at the molecular level. To characterize Pax6-mediated regulation of gene expression during murine forebrain neurogenesis, we performed microarray analysis with tissue from the dorsal Pax6-dependent telencephalon and the ventral Pax6-negative telencephalon at the onset of neurogenesis (E12) and at mid-neurogenesis (E15) in wild-type and Pax6-deficient mutant littermates. In the Pax6-deficient cortex the expression levels of various transcription factors involved in neurogenesis (like Satb2, Nfia, AP-2gamma, NeuroD6, Ngn2, Tbr2, Bhlhb5) and the retinoic acid signalling molecule Rlbp1 were reduced. Regulation by Pax6 could be confirmed upon electroporation of a Pax6- and a dominant-negative Pax6-containing vector into embryonic cortex. Taken together, our data reveal novel insights into the molecular pathways regulated by Pax6 during cortical neurogenesis. Most intriguingly, this analysis revealed time- and region-specific differences in Pax6-mediated transcription, explaining the specific function of Pax6 at early and later stages of neurogenesis. AU - Holm, P.C. AU - Mader, M.T. AU - Haubst, N. AU - Wizenmann, A. AU - Sigvardsson, M.* AU - Götz, M. C1 - 4881 C2 - 24388 SP - 99-119 TI - Loss- and gain-of-function analyses reveal targets of Pax6 in the developing mouse telencephalon. JO - Mol. Cell. Neurosci. VL - 34 PB - Elsevier PY - 2007 SN - 1044-7431 ER - TY - JOUR AU - Borgkvist, A.* AU - Puelles, E.* AU - Carta, M.* AU - Acampora, D.* AU - Ang, S.-L.* AU - Wurst, W. AU - Goiny, M.* AU - Fisone, G.* AU - Simeone, A.* AU - Usiello, A.* C1 - 3724 C2 - 23956 SP - 293-302 TI - Altered dopaminergic innervation and amphetamine response in adult Otx2 conditional mutant mice. JO - Mol. Cell. Neurosci. VL - 31 PY - 2006 SN - 1044-7431 ER - TY - JOUR AU - Andrae, J.* AU - Afink, G.* AU - Zhang, X.-Q.* AU - Wurst, W. AU - Nister, M.* C1 - 4071 C2 - 22419 SP - 308-321 TI - Forced expression of platelet-derived growth factor B in the mouse cerebellar primordium changes cell migration during midline fusion and causes cerebellar ectopia. JO - Mol. Cell. Neurosci. VL - 26 PY - 2004 SN - 1044-7431 ER - TY - JOUR AU - Hack, M.A. AU - Sugimori, M.* AU - Lundberg, C.* AU - Nakafuku, M.* AU - Götz, M. C1 - 4621 C2 - 22433 SP - 664-678 TI - Regionalization and fate specification in neurospheres: The role of Olig2 and Pax6. JO - Mol. Cell. Neurosci. VL - 25 PY - 2004 SN - 1044-7431 ER - TY - JOUR AU - Panhuysen, M. AU - Vogt Weisenhorn, D.M. AU - Blanquet, V. AU - Brodski, C. AU - Heinzmann, U. AU - Beisker, W. AU - Wurst, W. C1 - 4072 C2 - 22420 SP - 101-111 TI - Effects of Wnt1 signaling on proliferation in the developing mid-/hindbrain region. JO - Mol. Cell. Neurosci. VL - 26 PY - 2004 SN - 1044-7431 ER -