TY - JOUR AU - Keymer, A.* AU - Cardoso, C.* AU - Messerer, M. AU - Brands, M.* AU - Banhara, A.* AU - Parniske, M.* AU - Mayer, K.F.X. AU - Doermann, P.* AU - Gutjahr, C.* C1 - 57230 C2 - 47634 CY - 3340 Pilot Knob Road, St Paul, Mn 55121 Usa SP - 129-129 TI - Arabidopsis lipid biosynthesis genes required for successful colonization by the pathogenic oomycete Hyaloperonospora arabidopsidis. JO - Mol. Plant Microbe Interact. VL - 32 IS - 10 PB - Amer Phytopathological Soc PY - 2019 SN - 0894-0282 ER - TY - JOUR AU - Kutschera, A.* AU - Dawid, C.* AU - Gisch, N.* AU - Schmid, C.* AU - Raasch, L.* AU - Gerster, T.* AU - Schaeffer, M.* AU - Smakowska, E.* AU - Belkhadir, Y.* AU - Vlot, A.C. AU - Chandler, C.E.* AU - Schellenberger, R.* AU - Schwudke, D.* AU - Ernst, R.K.* AU - Dorey, S.* AU - Hueckelhoven, R.* AU - Hofmann, T.* AU - Ranf, S.* C1 - 57229 C2 - 47648 CY - 3340 Pilot Knob Road, St Paul, Mn 55121 Usa SP - 241-242 TI - Bacterial medium chain 3-hydroxy fatty acid metabolites trigger LORE-mediated immunity in Arabidopsis thaliana. JO - Mol. Plant Microbe Interact. VL - 32 IS - 10 PB - Amer Phytopathological Soc PY - 2019 SN - 0894-0282 ER - TY - JOUR AB - Pipecolic acid (Pip) is an essential component of systemic acquired resistance, priming resistance in Arabidopsis thaliana against (hemi) biotrophic pathogens. Here, we studied the potential role of Pip in bacteria-induced systemic immunity in barley. Exudates of barley leaves infected with the systemic immunity-inducing pathogen Pseudomonas syringae pv. japonica induced immune responses in A. thaliana. The same leaf exudates contained elevated Pip levels compared with those of mock-treated barley leaves. Exogenous application of Pip induced resistance in barley against the hemibiotrophic bacterial pathogen Xanthomonas translucens pv. cerealis. Furthermore, both a systemic immunity-inducing infection and exogenous application of Pip enhanced the resistance of barley against the biotrophic powdery mildew pathogen Blumeria graminis f. sp. hordei. In contrast to a systemic immunity-inducing infection, Pip application did not influence lesion formation by a systemically applied inoculum of the necrotrophic fungus Pyrenophora teres. Nitric oxide (NO) levels in barley leaves increased after Pip application. Furthermore, X. translucens pv. cerealis induced the accumulation of superoxide anion radicals and this response was stronger in Pip-pretreated compared with mock-pretreated plants. Thus, the data suggest that Pip induces barley innate immune responses by triggering NO and priming reactive oxygen species accumulation. AU - Lenk, M. AU - Wenig, M. AU - Bauer, K. AU - Hug, F. AU - Knappe, C. AU - Lange, B. AU - Timsy, T. AU - Häußler, F. AU - Mengel, F. AU - Dey, S. AU - Schäffner, A. AU - Vlot, A.C. C1 - 56325 C2 - 47000 CY - 3340 Pilot Knob Road, St Paul, Mn 55121 Usa SP - 1303-1313 TI - Pipecolic acid is induced in barley upon infection and triggers immune responses associated with elevated nitric oxide accumulation. JO - Mol. Plant Microbe Interact. VL - 32 IS - 10 PB - Amer Phytopathological Soc PY - 2019 SN - 0894-0282 ER - TY - JOUR AU - Lenk, M. AU - Wenig, M. AU - Knappe, C. AU - Dey, S. AU - Stuttmann, J.* AU - Lang, D. AU - Fischer, I. AU - Imani, J.* AU - Kogel, K.H.* AU - Mayer, K.F.X. AU - Vlot, A.C. C1 - 57225 C2 - 47644 CY - 3340 Pilot Knob Road, St Paul, Mn 55121 Usa SP - 20-20 TI - High throughput phenotyping to optimize CRISPR/Cas9 mutation efficiency in cereal crops. JO - Mol. Plant Microbe Interact. VL - 32 IS - 10 PB - Amer Phytopathological Soc PY - 2019 SN - 0894-0282 ER - TY - JOUR AU - Stabl, G.* AU - Schalk, S.* AU - Siani, R. AU - Schloter, M. AU - Dawid, C.* AU - Gutjahr, C.* C1 - 57228 C2 - 47647 CY - 3340 Pilot Knob Road, St Paul, Mn 55121 Usa SP - 54-54 TI - Deciphering the influence of arbuscular mycorrhiza on the root exudate and microbiome of Lotus japonicus. JO - Mol. Plant Microbe Interact. VL - 32 IS - 10 PB - Amer Phytopathological Soc PY - 2019 SN - 0894-0282 ER - TY - JOUR AU - Thind, A.K.* AU - Wicker, T.* AU - Müller, T.* AU - Ackermann, P.M.* AU - Steuernagel, B.* AU - Wulff, B.B.* AU - Spannagl, M. AU - Felder, M. AU - Lux, T. AU - Mayer, K.F.X. AU - Keller, B.* AU - Krattinger, S.G.* C1 - 57223 C2 - 47655 CY - 3340 Pilot Knob Road, St Paul, Mn 55121 Usa SP - 180-181 TI - Chromosome scale comparative analysis of two wheat cultivars unravels large structural variations and underpinning molecular mechanisms. JO - Mol. Plant Microbe Interact. VL - 32 IS - 10 PB - Amer Phytopathological Soc PY - 2019 SN - 0894-0282 ER - TY - JOUR AU - Wenig, M. AU - Ghirardo, A. AU - Sales, J. AU - Schnitzler, J.-P. AU - Vlot, A.C. C1 - 57222 C2 - 47654 CY - 3340 Pilot Knob Road, St Paul, Mn 55121 Usa SP - 238-238 TI - Monoterpenes act as volatile cues in intra-plant and inter-plant propagation of innate immunity. JO - Mol. Plant Microbe Interact. VL - 32 IS - 10 PB - Amer Phytopathological Soc PY - 2019 SN - 0894-0282 ER - TY - JOUR AB - Esca disease is one of the major grapevine trunk diseases in Europe and the aetioloy is complex since several inhabiting fungi are identified to be associated with this disease. Among the foliar symptom expression, apoplectic form may be distinguished and characterized by sudden dieback of shoots, leaf drop and shriveling of grape clusters in few days that can ultimately induce the plant death. To further understand this drastic event, we conducted transcriptomic and metabolomic analyses to characterize responses of leaves during the period preceding symptom appearance (twenty and seven days before foliar symptoms expression) and at the day of apoplexy expression. Transcriptomic and metabolomic provide signatures for the apoplectic leaves and most of changes concern the metabolism of carbohydrates, amino acids, and phenylpropanoids. Deciphering on glutathione-S-transferase, its preferential location in phloem, correlated with the upregulation of GST genes and a decrease of the glutathione level, offer further support to the putative role of glutathione during apoplexy expression. AU - Magnin-Robert, M.* AU - Adrian, M.V.* AU - Trouvelot, S.* AU - Spagnolo, A.* AU - Jacquens, L.* AU - Letousey, P.* AU - Rabenoelina, F.* AU - Harir, M. AU - Roullier-Gall, C. AU - Clément, C.* AU - Schmitt-Kopplin, P. AU - Vallat, A.* AU - Abou-Mansour, E.* AU - Fontaine, F.* C1 - 51756 C2 - 43510 CY - St Paul SP - 946-959 TI - Alterations in grapevine leaf metabolism occur prior to esca apoplexy appearance. JO - Mol. Plant Microbe Interact. VL - 30 IS - 12 PB - Amer Phytopathological Soc PY - 2017 SN - 0894-0282 ER - TY - JOUR AB - The commercially available inoculant Bacillus amyloliquefaciens FZB42 is able to considerably reduce lettuce bottom rot caused by Rhizoctonia solani. To understand the interaction between FZB42 and R. solani in the rhizosphere of lettuce we used an axenic system with lettuce bacterized with FZB42, and inoculated with R. solani. Confocal laser scanning microscopy showed that FZB42 could delay the initial establishment of R. solani on the plants. To show which secondary metabolites of FZB42 are produced under these in-situ conditions we developed an UHPLC-qToF-MS based method and identified surfactin, fengycin and bacillomycin D in the lettuce rhizosphere. We hypothesized that lipopeptides and polyketides play a role in enhancing the plant defence responses in addition to the direct antagonistic effect towards R. solani , and used a qRT-PCR based assay for marker genes involved in defence signaling pathways in lettuce. A significant higher expression of PDF 1.2 observed in the bacterized plants in response to subsequent pathogen challenge showed that FZB42 could enhance the lettuce defence response towards the fungal pathogen. To identify if surfactin or other non-ribosomally synthesized secondary metabolites could elicit the observed enhanced defence gene expression, we examined two mutants of FZB42 deficient in production of surfactin and the lipopetides and polyketides by expression analysis and pot experiments. In the absence of surfactin and other non-ribosomally synthesized secondary metabolites there was no enhanced PDF 1.2 mediated response to the pathogen challenge. Pot experiment results showed that the mutants failed to reduce disease incidence in lettuce as compared to the FZB42 wild type, indicating, that surfactin as well as other non-ribosomally synthesized secondary metabolites play a role in the actual disease suppression and on lettuce health. In conclusion, our study showed that non-ribosomally synthesized secondary metabolites of FZB42 are actually produced in the lettuce rhizosphere and contribute to the disease suppression by mediating plant defence gene expression towards the pathogen R. solani. AU - Chowdhury, S.P. AU - Uhl, J. AU - Grosch, R.* AU - Alqueres, S. AU - Pittroff, S. AU - Dietel, K.* AU - Schmitt-Kopplin, P. AU - Borriss, R.* AU - Hartmann, A. C1 - 44976 C2 - 37137 SP - 984-995 TI - Cyclic lipopeptides of Bacillus amyloliquefaciens subsp. plantarum colonizing the lettuce rhizosphere enhance plant defence responses towards the bottom rot pathogen Rhizoctonia solani. JO - Mol. Plant Microbe Interact. VL - 28 IS - 9 PY - 2015 SN - 0894-0282 ER - TY - JOUR AB - Gluconacetobacter diazotrophicus is an aerobic diazotrophic plant-growth-promoting bacterium isolated from different gramineous plants. We showed that reactive oxygen species (ROS) were produced at early stages of rice root colonization, a typical plant defense response against pathogens. The transcription of the pathogen-related-10 gene of the jasmonic acid (JA) pathway but not of the PR-1 gene of the salicylic acid pathway was activated by the endophytic colonization of rice roots by G. diazotrophicus strain PALS. Quantitative polymerase chain reaction analyses showed that, at early stages of colonization, the bacteria upregulated the transcript levels of ROS-detoxifying genes such as superoxide dismutase (SOD) and glutathione reductase (GR). To proof the role of ROS-scavenging enzymes in the colonization and interaction process, transposon insertion mutants of the SOD and GR genes of strain PALS were constructed. The SOD and GR mutants were unable to efficiently colonize the roots, indicated by the decrease of tightly root-associated bacterial cell counts and endophytic colonization and by fluorescence in situ hybridization analysis. Interestingly, the mutants did not induce the PR-10 of the JA-pathway, probably due to the inability of endophytic colonization. Thus, ROS-scavenging enzymes of G. diazotrophicus strain PALS play an important role in the endophytic colonization of rice plants. AU - Alqueres, S. AU - Meneses, C.* AU - Rouws, L.* AU - Rothballer, M. AU - Baldani, I.* AU - Schmid, M. AU - Hartmann, A. C1 - 26720 C2 - 32360 SP - 937-945 TI - The bacterial superoxide dismutase and glutathione reductase are crucial for endophytic colonization of rice roots by Gluconacetobacter diazotrophicus PAL5. JO - Mol. Plant Microbe Interact. VL - 26 IS - 8 PB - Amer. Phytopathological Soc. PY - 2013 SN - 0894-0282 ER - TY - JOUR AB - Plant small molecule UDP-glycosyltransferases (UGT) glycosylate a vast number of endogenous substances but also act in detoxification of metabolites produced by plant pathogenic microorganisms. The ability to inactivate the Fusarium graminearum mycotoxin deoxynivalenol (DON) into DON-3-O-glucoside is crucial for resistance of cereals. We analyzed the UGT gene family of the monocot model species Brachypodium distachyon and functionally characterized two gene clusters containing putative orthologs of previously identified DON-detoxification genes from Arabidopsis thaliana and barley. Analysis of transcription showed that UGTs encoded in both clusters are highly inducible by DON and expressed at much higher levels upon infection with a wild-type DON-producing F. graminearum strain compared to infection with a mutant deficient in DON production. Expression of these genes in a toxin sensitive strain of Saccharomyces cerevisiae revealed that only two B. distachyon UGTs encoded by members of a cluster of six genes homologous to the DON-inactivating barley HvUGT13248 were able to convert DON into DON-3-O-glucoside. Also, a single copy gene from Sorghum bicolor orthologous to this cluster and one of three putative orthologs of rice exhibit this ability. Seemingly, the UGT genes undergo rapid evolution and changes in copy number, making it difficult to identify orthologs with conserved substrate specificity. AU - Schweiger, W.* AU - Pasquet, J.C.* AU - Nussbaumer, T. AU - Kovalsky Paris, M.P.* AU - Wiesenberger, G.* AU - Macadré, C.* AU - Ametz, C.* AU - Berthiller, F.* AU - Lemmens, M.* AU - Saindrenan, P.* AU - Mewes, H.-W. AU - Mayer, K.F.X. AU - Dufresne, M.* AU - Adam, G.* C1 - 23803 C2 - 31294 SP - 781-792 TI - Functional characterization of two clusters of Brachypodium distachyon UDP-glycosyltransferases encoding putative deoxynivalenol detoxification genes. JO - Mol. Plant Microbe Interact. VL - 26 IS - 7 PB - Amer. Phytopathological Soc. PY - 2013 SN - 0894-0282 ER - TY - JOUR AU - Raacke, I.C.* AU - von Rad, U. AU - Mueller, M.J.* AU - Berger, S.* C1 - 3027 C2 - 23859 SP - 1138-1146 TI - Yeast increases resistance in Arabidopsis against Pseudomonas syringae and Botrytis cinerea by salicylic acid-dependent as well as -independent mechanisms. JO - Mol. Plant Microbe Interact. VL - 19 PY - 2006 SN - 0894-0282 ER - TY - JOUR AB - Harpin is a well-known proteinaceous bacterial elicitor that can induce an oxidative burst and programmed cell death in various host plants. Given the demonstrated roles of mitochondria in animal apoptosis, we investigated the effect of harpin from Pseudomonas syringae on mitochondrial functions in Arabidopsis suspension cells in detail. Fluorescence microscopy in conjunction with double-staining for reactive oxygen species (ROS) and mitochondria suggested co-localization of mitochondria and ROS generation. Plant defense responses or cell death after pathogen attack have been suggested to be regulated by the concerted action of ROS and nitric oxide (NO). However, although Arabidopsis cells respond to harpin treatment with NO generation, time course analyses suggest that NO generation is not involved in initial responses but, rather, is a consequence of cellular decay. Among the fast responses we observed was a decrease of the mitochondrial membrane potential deltapsim, and, possibly as a direct consequence, of ATP production. Furthermore, treatment of Arabidopsis cells with harpin protein induced a rapid cytochrome C release from mitochondria into the cytosol, which is regarded as a hallmark of programmed cell death or apoptosis. Northern and DNA array analyses showed strong induction of protecting or scavenging systems such as alternative oxidase and small heat shock proteins, components that are known to be associated with cellular stress responses. In sum, the presented data suggest that harpin inactivates mitochondria in Arabidopsis cells. AU - Krause, M. AU - Durner, J. C1 - 3471 C2 - 21703 SP - 131-139 TI - Harpin inactivates mitochondria in arabidopsis suspension cells. JO - Mol. Plant Microbe Interact. VL - 17 IS - 2 PB - APS PY - 2004 SN - 0894-0282 ER -