TY  - JOUR
AB  - Owing to reduced light scattering and tissue autofluorescence, in vivo fluorescence imaging in the 1,000–3,000-nm near-infrared II (NIR-II) spectral range can afford non-invasive imaging at depths of millimetres within biological tissue. Infrared fluorescent probes labelled with antibodies or other targeting ligands also enable NIR-II molecular imaging at the single-cell level. Here we present recent developments in the design of fluorophores and probes emitting in the NIR-II window based on organic synthesis and nanoscience approaches. We also review advances in NIR-II wide-field and microscopy imaging modalities, with a focus on preclinical imaging and promising clinical translation case studies. Finally, we outline current issues and challenges for the wider adoption of NIR-II imaging in biomedical research and clinical imaging.
AU  - Wang, F.*
AU  - Zhong, Y.*
AU  - Bruns, O.T.
AU  - Liang, Y.*
AU  - Dai, H.*
C1  - 70285
C2  - 55485
CY  - Heidelberger Platz 3, Berlin, 14197, Germany
TI  - In vivo NIR-II fluorescence imaging for biology and medicine.
JO  - Nat. Photonics
PB  - Nature Portfolio
PY  - 2024
SN  - 1749-4885
ER  - 

TY  - JOUR
AB  - Correction to: Nature Photonicshttps://doi.org/10.1038/s41566-024-01391-5, published online 4 March 2024 In the version of the article initially published, the credit for Fig. 1d was incorrect and has now been amended to Guosong Hong, Stanford University in the HTML and PDF versions of the article.
AU  - Wang, F.*
AU  - Zhong, Y.*
AU  - Bruns, O.T.
AU  - Liang, Y.*
AU  - Dai, H.*
C1  - 70797
C2  - 55679
TI  - Author Correction: In vivo NIR-II fluorescence imaging for biology and medicine (Nature Photonics, (2024), 10.1038/s41566-024-01391-5).
JO  - Nat. Photonics
PY  - 2024
SN  - 1749-4885
ER  - 

TY  - JOUR
AB  - Ultrasonography1 and photoacoustic2,3 (optoacoustic) tomography have recently seen great advances in hardware and algorithms. However, current high-end systems still use a matrix of piezoelectric sensor elements, and new applications require sensors with high sensitivity, broadband detection, small size and scalability to a fine-pitch matrix. This work demonstrates an ultrasound sensor in silicon photonic technology with extreme sensitivity owing to an innovative optomechanical waveguide. This waveguide has a tiny 15 nm air gap between two movable parts, which we fabricated using new CMOS-compatible processing. The 20 μm small sensor has a noise equivalent pressure below 1.3 mPa Hz−1/2 in the measured range of 3–30 MHz, dominated by acoustomechanical noise. This is two orders of magnitude better than for piezoelectric elements of an identical size4. The demonstrated sensor matrix with on-chip photonic multiplexing5–7 offers the prospect of miniaturized catheters that have sensor matrices interrogated using just a few optical fibres, unlike piezoelectric sensors that typically use an electrical connection for each element.
AU  - Westerveld, W.J.*
AU  - Mahmud-Ul-Hasan, M.*
AU  - Shnaiderman, R.
AU  - Ntziachristos, V.
AU  - Rottenberg, X.*
AU  - Severi, S.*
AU  - Rochus, V.*
C1  - 61612
C2  - 50351
CY  - Heidelberger Platz 3, Berlin, 14197, Germany
SP  - 341–345
TI  - Sensitive, small, broadband and scalable optomechanical ultrasound sensor in silicon photonics.
JO  - Nat. Photonics
VL  - 15
PB  - Nature Research
PY  - 2021
SN  - 1749-4885
ER  - 

TY  - JOUR
AB  - The emerging clinical use of targeted fluorescent agents heralds a shift in intraoperative imaging practices that overcome the limitations of human vision. However, in contrast to established radiological methods, no appropriate performance specifications and standards have been established in fluorescence molecular imaging. Moreover, the dependence of fluorescence signals on many experimental parameters and the use of wavelengths ranging from the visible to short-wave infrared (400–1,700 nm) complicate quality control in fluorescence molecular imaging. Here, we discuss the experimental parameters that critically affect fluorescence molecular imaging accuracy, and introduce the concept of high-fidelity fluorescence imaging as a means for ensuring reliable reproduction of fluorescence biodistribution in tissue.
AU  - Koch, M.
AU  - Symvoulidis, P.
AU  - Ntziachristos, V.
C1  - 54231
C2  - 45335
CY  - Macmillan Building, 4 Crinan St, London N1 9xw, England
SP  - 505-515
TI  - Tackling standardization in fluorescence molecular imaging.
JO  - Nat. Photonics
VL  - 12
IS  - 9
PB  - Nature Publishing Group
PY  - 2018
SN  - 1749-4885
ER  - 

TY  - JOUR
AB  - Optoacoustic imaging, or photoacoustic imaging, is insensitive to photon scattering within biological tissue and, unlike conventional optical imaging methods, makes high-resolution optical visualization deep within tissue possible. Recent advances in laser technology, detection strategies and inversion techniques have led to significant improvements in the capabilities of optoacoustic systems. A key empowering feature is the development of video-rate multispectral imaging in two and three dimensions, which offers fast, spectral differentiation of distinct photoabsorbing moieties. We review recent advances and capabilities in the technology and its corresponding emerging biological and clinical applications.
AU  - Taruttis, A.*
AU  - Ntziachristos, V.
C1  - 44417
C2  - 36937
CY  - London
SP  - 219-227
TI  - Advances in real-time multispectral optoacoustic imaging and its applications.
JO  - Nat. Photonics
VL  - 9
IS  - 4
PB  - Nature Publishing Group
PY  - 2015
SN  - 1749-4885
ER  - 

TY  - JOUR
AB  - Fluorescent proteins have become essential reporter molecules for studying life at the cellular and sub-cellular level, re-defining the ways in which we investigate biology. However, because of intense light scattering, most organisms and tissues remain inaccessible to current fluorescence microscopy techniques at depths beyond several hundred micrometres. We describe a multispectral opto-acoustic tomography technique capable of high-resolution visualization of fluorescent proteins deep within highly light-scattering living organisms. The method uses multiwavelength illumination over multiple projections combined with selective-plane opto-acoustic detection for artifact-free data collection. Accurate image reconstruction is enabled by making use of wavelength-dependent light propagation models in tissue. By performing whole-body imaging of two biologically important and optically diffuse model organisms, Drosophila melanogaster pupae and adult zebrafish, we demonstrate the facility to resolve tissue-specific expression of eGFP and mCherrry fluorescent proteins for precise morphological and functional observations in vivo.
AU  - Razansky, D.
AU  - Distel, M.
AU  - Vinegoni, C.*
AU  - Ma, R.*
AU  - Perrimon, N.*
AU  - Köster, R.W.
AU  - Ntziachristos, V.
C1  - 514
C2  - 26996
SP  - 412-417
TI  - Multispectral opto-acoustic tomography of deep-seated fluorescent proteins in vivo.
JO  - Nat. Photonics
VL  - 3
IS  - 7
PB  - Nature Publ. Group
PY  - 2009
SN  - 1749-4885
ER  -