TY  - JOUR
AB  - State-of-the-art tissue-clearing methods provide subcellular-level optical access to intact tissues from individual organs and even to some entire mammals. When combined with light-sheet microscopy and automated approaches to image analysis, existing tissue-clearing methods can speed up and may reduce the cost of conventional histology by several orders of magnitude. In addition, tissue-clearing chemistry allows whole-organ antibody labelling, which can be applied even to thick human tissues. By combining the most powerful labelling, clearing, imaging and data-analysis tools, scientists are extracting structural and functional cellular and subcellular information on complex mammalian bodies and large human specimens at an accelerated pace. The rapid generation of terabyte-scale imaging data furthermore creates a high demand for efficient computational approaches that tackle challenges in large-scale data analysis and management. In this Review, we discuss how tissue-clearing methods could provide an unbiased, system-level view of mammalian bodies and human specimens and discuss future opportunities for the use of these methods in human neuroscience.Tissue-clearing methods are now allowing 3D imaging of intact tissues and some entire mammals. In this Review, Ueda and colleagues discuss the various tissue-clearing methods, related techniques and data analysis and management, as well as the application of these methods in neuroscience.
AU  - Ueda, H.R.*
AU  - Ertürk, A.
AU  - Chung, K.*
AU  - Gradinaru, V.*
AU  - Chedotal, A.*
AU  - Tomancak, P.*
AU  - Keller, P.J.*
C1  - 57756
C2  - 48116
CY  - Macmillan Building, 4 Crinan St, London N1 9xw, England
SP  - 61-79
TI  - Tissue clearing and its applications in neuroscience.
JO  - Nat. Rev. Neurosci.
VL  - 21
IS  - 2
PB  - Nature Publishing Group
PY  - 2020
SN  - 1471-003X
ER  - 

TY  - JOUR
AB  - In this Review Article, the accept date was inadvertently omitted. It should be 18 November 2019. This error has been corrected online. https://doi.org/10.1038/s41583-020-0291-5 | Published online 09 March 2020.
AU  - Ueda, H.R.*
AU  - Ertürk, A.
AU  - Gradinaru, V.*
AU  - Chédotal, A.*
AU  - Tomancak, P.*
AU  - Keller, P.J.*
C1  - 58549
C2  - 48153
TI  - Publisher Correction: Tissue clearing and its applications in neuroscience (Nature Reviews Neuroscience, (2020), 21, 2, (61-79), 10.1038/s41583-019-0250-1).
JO  - Nat. Rev. Neurosci.
PY  - 2020
SN  - 1471-003X
ER  - 

TY  - JOUR
AB  - Astrocyte-like cells, which act as stem cells in the adult brain, reside in a few restricted stem cell niches. However, following brain injury, glia outside these niches acquire or reactivate stem cell potential as part of reactive gliosis. Recent studies have begun to uncover the molecular pathways involved in this process. A comparison of molecular pathways activated after injury with those involved in the normal neural stem cell niches highlights strategies that could overcome the inhibition of neurogenesis outside the stem cell niche and instruct parenchymal glia towards a neurogenic fate. This new view on reactive glia therefore suggests a widespread endogenous source of cells with stem cell potential, which might potentially be harnessed for local repair strategies.
AU  - Robel, S.*
AU  - Berninger, B.*
AU  - Götz, M.
C1  - 5938
C2  - 28416
CY  - London
SP  - 88-104
TI  - The stem cell potential of glia: Lessons from reactive gliosis.
JO  - Nat. Rev. Neurosci.
VL  - 12
IS  - 2
PB  - Nature Publ. Group
PY  - 2011
SN  - 1471-003X
ER  - 

TY  - JOUR
AU  - Wurst, W.
AU  - Bally-Cuif, L.
C1  - 9710
C2  - 20768
SP  - 99-108
TI  - Neural plate pattering : Upstream and downstream of the isthmic iorganizer. 
JO  - Nat. Rev. Neurosci.
VL  - 2
PY  - 2001
SN  - 1471-003X
ER  -