TY - JOUR AB - Intestinal organoids reflect the 3D structure and function of their original tissues. Organoid are typically cultured in Matrigel, an extracellular matrix (ECM) mimicking the basement membrane, which is suitable for epithelial cells but does not accurately mimic the tumour microenvironment of colorectal cancer (CRC). The ECM and particularly collagen type I is crucial for CRC progression and invasiveness. Given that efforts to examine CRC organoid invasion in a more physiologically relevant ECM have been limited, we used a floating collagen type I matrix (FC) to study organoid invasion in three patient-derived CRC organoid lines. In FC gel, organoids contract, align, and fuse into macroscopic ring structures, initiating minor branch formation and invasion fronts, phenomena unique for the collagen ECM and otherwise not observed in Matrigel-grown CRC organoids. In contrast to Matrigel, FC organoids showed basal extrusion with improper actin localization, but without change in the organoid polarity. Moreover, small clusters of vital invading cells were observed. Gene expression analysis revealed that the organoids cultured in a FC matrix presented more epithelial and stem cell-like characteristics. This novel technique of cultivating CRC organoids in a FC matrix represents an in-vitro model for studying cancer organization and matrix remodelling with increased organoid stemness potential. AU - Wimmers, D.G.* AU - Huebner, K.* AU - Dale, T.* AU - Papargyriou, A. AU - Reichert, M. AU - Hartmann, A.* AU - Schneider-Stock, R.* C1 - 73674 C2 - 57164 CY - Hackerbrucke 6, 80335 Munich, Germany TI - A floating collagen matrix triggers ring formation and stemness characteristics in human colorectal cancer organoids. JO - Pathol. Res. Pract. VL - 269 PB - Elsevier Gmbh PY - 2025 SN - 0344-0338 ER - TY - JOUR AU - Romeike, B.F.M.* AU - Böckeler, A.* AU - Kremmer, E. AU - Sommer, P.* AU - Grässer, F.* AU - Krick, C.* C1 - 2272 C2 - 23310 SP - 727-732 TI - Immunohistochemical detection of dITPase in intracranial tumors. JO - Pathol. Res. Pract. VL - 201 PY - 2005 SN - 0344-0338 ER - TY - JOUR AU - Fend, F.* AU - Kremer, M.* AU - Specht, K.* AU - Quintanilla-Martinez, L. C1 - 9953 C2 - 21175 SP - 425-430 TI - Laser Microdissection in Hematopathology. JO - Pathol. Res. Pract. VL - 199 PY - 2003 SN - 0344-0338 ER - TY - JOUR AB - Interactive selection of a limited number of cells in imaging cytometry for determining the DNA histogram of breast cancer cells as the best known prognosticator at the moment, implies statistical and systematic sampling problems. Analysis of histograms of 361 breast cancer aspirate specimens measured in two laboratories demonstrate the expected high statistical variations in view of the only 100 cells measured per case but also slight systematic differences. Controlled systematic sampling without pathological bias results in a somewhat higher malignancy grading than selective biased sampling. For this finding we have no explanation. The main result is, however, that we did not find the expected contrary which makes the argument for at least this application invalid that expert pathologists are needed for reliable interactive sampling. AU - Burger, G.T. AU - Aubele, M. AU - Jütting, U. AU - Auer, G.U. C1 - 40550 C2 - 0 SP - 391-395 TI - Interactive cytometry, chance or evil of bias?. JO - Pathol. Res. Pract. VL - 188 IS - 4-5 PY - 1992 SN - 0344-0338 ER - TY - JOUR AU - Dörmer, P. AU - Hültner, L. AU - Mergenthaler, H.-G. C1 - 18047 C2 - 10954 SP - 145-149 TI - Proliferation and Maturation of Human Bone Marrow Cells in Infectious Diseases. JO - Pathol. Res. Pract. VL - 186 PY - 1990 SN - 0344-0338 ER - TY - JOUR AB - In 6 patients with various types of infectious disease an extendced study of proliferation and maturation of erythropoiesis and granulocytopoiesis was performed. By means of quantitative 14C-autoradiography DNA synthesis time and labeling index were determined in every morphologically defined cell compartment of both lineages. With these parameters and the relative frequency of cells in the various compartments cell cycle times, relative cell production rates and maturation indices were determined. A general labeling index reduction and prolongation of DNA synthesis time was observed which was statistically significant in the majority of compartments. As a consequence, cell cycle times were prolonged throughout, the deviation from normal increasing with advancing maturation in both lineages. Relative cell production was normal in myelocytes, but elevated in myeloblasts and promyelocytes. On the other hand, proerythroblasts and basophilic erythroblasts showed normal relative cell production rates, while a significantly reduced value was found in polychromatic erythroblasts. The maturation index in both lineages was reduced by roughly 50%. Since cell cycle times were generally prolonged, the most significant deviations from normal being present in the latest proliferative compartments, the low maturation indices are discussed in the light of ineffective erythro- and granulocytopoiesis. Premature cell death in the bone marrow is suggested to be a significant factor in particular cases of infectious disease. AU - Dörmér, P.G. AU - Hültner, L. AU - Mergenthaler, H.G. C1 - 42628 C2 - 0 SP - 145-149 TI - Proliferation and maturation of human bone marrow cells in infectious diseases. JO - Pathol. Res. Pract. VL - 186 IS - 1 PY - 1990 SN - 0344-0338 ER - TY - JOUR AB - Glass models of small rectangularly branched and 360° curved arteries have been perfused with diluted bovine platelet rich plasma activated with ADP. Whereas straight tube flow did not lead to platelet deposits, branched tube flow caused thrombi at two sites. First site was the region of the distal rim of the orifice of the side branch, second site was the wall of the main tube opposite and downstream the side branch origin. Curved tube flow produced deposits on the wall with the smaller radius and on the wall with the greater radius of curvature. The formation of deposits at the specific sites required definite flow conditions. Thrombi at sites corresponding to the predilection sites of atherosclerosis in small arteries, i.e. the wall opposite the side branch and the wall with the smaller radius of curvature, occured where flow separated from the wall, reattaches forming a stagnation point flow. The influence of stagnation point flows on thrombus formation on glass walls was studied quantitatively for a rotationally symmetric stagnation point flow. The experiments revealed that, activated bovine platelets adhere only where they are transported by flows to the wall exhibiting velocity components directed perpendicularly towards the wall; polycellular stripe like thrombi form only when the velocity components of platelets parallel to the wall overcomes a critical value; the mass of deposited polycellular thrombi increases with the velocity component parallel to the wall. For all deposits a critical parallel velocity component exists which dislodges thrombi. AU - Baldauf, W. AU - Wurzinger, L.J. AU - Kinder, J. C1 - 42364 C2 - 35828 SP - 9-33 TI - The role of stagnation point flow in the formation of platelet thrombi on glass surfaces in tubes with various geometry. JO - Pathol. Res. Pract. VL - 163 IS - 1 PY - 1978 SN - 0344-0338 ER -