TY  - JOUR
AB  - mmune mediated keratitis (IMMK) is primarily a non-ulcerative keratitis in horses causing intermittent ocular pain, eventually resulting in visual impairment. Affected horses typically respond to immunomodulatory treatment. However, the underlying cause of the disease remains enigmatic. The current study was undertaken to investigate the presence of autoantibodies in horses with immune mediated keratitis. Using 28 horses with IMMK and 27 healthy controls screening for serum autoantibodies against the corneal proteome using indirect immunofluorescence, one-dimensional (1DE) and two-dimensional electrophoresis (2DE) with subsequent western blot analysis was performed followed by mass spectrometric identification of bands or spots of interest. Indirect immunofluorescence did not reveal a difference in immune response towards corneal proteins between healthy horses and those with IMMK. Using western blot analysis some horses affected by IMMK (4/28) showed a single band (1D) or a single spot (2DE) (5/28) not detected in healthy controls. The corresponding spot was identified as maspin (SERPINB5), a protein responsible for the inhibition of corneal vascularisation, cell migration and cell adhesion to the extracellular matrix. Tests with a recombinant human protein commercially available did not verify blot findings, but the human protein may not be fully cross-reactive. Still, maspin might play a role in some cases of equine IMMK. Further research is needed to clarify the etiology of this disease.
AU  - Braus, B.K.*
AU  - Miller, I.*
AU  - Kummer, S.*
AU  - Kleinwort, K.J.H.*
AU  - Hirmer, S.*
AU  - Hauck, S.M.
AU  - McMullen, R.J.*
AU  - Kerschbaumer, M.*
AU  - Deeg, C.A.*
C1  - 51069
C2  - 42806
CY  - Amsterdam
SP  - 48-54
TI  - Investigation of corneal autoantibodies in horses with immune mediated keratitis (IMMK).
JO  - Vet. Immunol. Immunopathol.
VL  - 187
PB  - Elsevier Science Bv
PY  - 2017
SN  - 0165-2427
ER  - 

TY  - JOUR
AB  - Human leukocyte antigen (HLA)-haploidentical stem cell transplantation is an opportunity for nearly all patients lacking an HLA matched stem cell donor. However, graft rejection and graft-versus-host disease (GvHD) as well as infectious complications still result in high treatment-related mortality. Here, we used the dog as a preclinical model for the study of tolerance induction with the aim to optimize and to improve a clinical protocol of haploidentical stem cell transplantation. For this purpose CD6-depleted peripheral blood stem cells (PBSCs) were transfused 6d after transplantation of unmodified bone marrow from dog leukocyte antigen (DLA)-haploidentical littermate donors in order to induce immune tolerance. Besides hematopoietic stem cells CD6-depleted PBSC contain, NK cells and a minority of suppressive CD8-positive cells that may suppress activated T lymphocytes. Recipients were conditioned with, cyclophosphamide and antithymocyte globulin (ATG) preceded by a transfusion of donor buffy coat and either 1, 2 or 3×3.3Gy total body irradiation (TBI). Postgrafting immunosuppression was limited to 30d of cyclosporine and methotrexate. The additional administration of CD6-depleted PBSCs after unmodified marrow could not prevent GvHD, but it may improve engraftment and chimerism after conditioning with 2×3.3Gy TBI. Reasons for incomplete suppression and possible improvements for clinical applications are discussed.
AU  - Zorn, J.
AU  - Schwamberger, S.
AU  - Panzer, W.
AU  - Adler, H.
AU  - Kolb, H.-J.
C1  - 6636
C2  - 29015
CY  - Amsterdam, Netherlands
SP  - 27-35
TI  - Transplantation of CD6-depleted peripheral blood stem cells after DLA-haploidentical bone marrow transplantation contributes to engraftment and tolerance in a preclinical model of stem cell transplantation.
JO  - Vet. Immunol. Immunopathol.
VL  - 144
IS  - 1-2
PB  - Elsevier
PY  - 2011
SN  - 0165-2427
ER  - 

TY  - JOUR
AB  - Sudden acquired retinal degeneration syndrome (SARDS) is a disease characterised by sudden and bilateral vision loss of dogs. Previous studies failed to identify the underlying cause [Mattson, A., Roberts, S.M., Isherwood, J.M.E., 1992. Clinical features suggesting hyperadrenocorticism associated with sudden acquired retinal degeneration syndrome in a dog. J. Am. Anim. Hosp. Assoc. 28, 199-202; Van der Woerdt, A., Nasisse, M.P., Davidson, M.G., 1991. Sudden acquired retinal degeneration in the dog: clinical and laboratory findings in 36 cases. Prog. Vet. Comp. Ophthamol. 1, 11-18] and earlier investigations about the occurrence of anti-retinal antibodies in SARDS patients showed inconsistent results. To provide a novel approach to those findings we designed a more detailed study. Autoantibodies of SARDS patients and normal controls were tested against the purified autoantigens S-antigen and cellular retinaldehyde binding protein (CRALBP) that play a role in human autoimmune uveitis. Next we tested the autoantibody binding pattern to whole retinal lysate. No difference in the incidence of autoantibodies could be found between SARDS patients and healthy controls while testing the well-known autoantigens S-antigen and CRALBP. Potential novel, yet unknown autoantigens were identified by a screening test using the retinal proteome as an autoantigenic source. In SARDS patients and normal controls, several retinal proteins were bound by IgG antibodies, but one band was strongly marked by SARDS patients. That band was excised, subjected to mass spectrometry (matrix-assisted laser desorption/ionisation-time of flight (MALDI-TOF/TOF)) and identified as neuron-specific enolase. Binding of the IgG autoantibodies of SARDS-affected dogs to this protein was verified using purified NSE, revealing 25% of NSE autoantibody-positive SARDS patients and 0% of negative controls. Our findings indicate that at least some dogs with SARDS have autoantibodies against NSE, although it is unclear whether these play a causative role in SARDS or whether they are the result of retinal destruction by another mechanism.
AU  - Braus, B.K.*
AU  - Hauck, S.M.
AU  - Amann, B.*
AU  - Heinrich, C.*
AU  - Fritsche, J.*
AU  - Koestlin, R.*
AU  - Deeg, C.A.*
C1  - 2986
C2  - 25852
SP  - 177-183
TI  - Neuron-specific enolase antibodies in patients with sudden acquired retinal degeneration syndrome.
JO  - Vet. Immunol. Immunopathol.
VL  - 124
IS  - 1-2
PB  - Elsevier Scientific Publ. Co.
PY  - 2008
SN  - 0165-2427
ER  - 

TY  - JOUR
AB  - The major goal of this work was to describe the in vitro generation of mature functional neutrophils derived from a canine enriched haematopoietic progenitor cell population. We have utilised lineage depletion by immunomagnetic selection to isolate a canine haematopoietic progenitor cell population. The physical, immunological, metabolical and morphological methodologies employed in this study have permitted us to isolate and define a cell population enriched in Rh-123low and CD34+ cells. Irradiated pre-established long-term bone marrow cultures (LTBMC) were utilised to determine the self-renewal ability of lineage negative (Lin−) cells, as well as their capacity to differentiate into mature functional neutrophils. The authors demonstrate for the first time that canine neutrophils derived from Lin− cells are able to produce oxyradicals, express a specific neutrophil surface antigen, and contain gelatinase granules. These characteristics enable them to migrate through basement membranes to act as a first line defence mechanism. The fact that these cells are able to differentiate into functional mature cells, and give rise to long-term culture-initiating cells (LTC-IC) after 35 days of culture, allows the authors to assure that the isolated canine enriched haematopoietic cell population exhibit functional characteristics, associated with primitive haematopoietic cells.
AU  - León, L.L.*
AU  - Ostronoff, L.K.
AU  - Fermin, M.L.*
AU  - Fragio, C.*
AU  - Kremmer, E.
AU  - Kolb, H.-J.
AU  - Tejero, C.
C1  - 1283
C2  - 26157
CY  - Amsterdam
SP  - 41-50
TI  - In vitro generation of mature neutrophils from canine Lin- bone marrow cells.
JO  - Vet. Immunol. Immunopathol.
VL  - 107
IS  - 1-2
PB  - Elsevier
PY  - 2005
SN  - 0165-2427
ER  - 

TY  - JOUR
AU  - Fiegler, H.
AU  - Knabel, M.
AU  - Franz, M.
AU  - Kolb, H.-J.
AU  - Just, U.
C1  - 21965
C2  - 20489
SP  - 61-70
TI  - Determination of donor-type chimerism using a semi-quantitative PCR-based method in a canine model for bone marrow transplantation.
JO  - Vet. Immunol. Immunopathol.
VL  - 84
PY  - 2002
SN  - 0165-2427
ER  - 

TY  - JOUR
AB  - CD3, CD4, CD5, and CD8 antigen expression of T cells and IgG expression of B cells and canine distemper virus (CDV) antigen distribution were immunohistochemically examined in lymphoid tissues (lymph node, spleen, thymus, and tonsil) of control dogs and animals with spontaneous canine distemper. In addition, CNS tissue of all animals was studied for neuropathological changes and CDV antigen distribution. Based on the degree of depletion distemper dogs were classified into two groups. Group I represented animals with moderate to marked lymphoid depletion, while group II dogs displayed mild or no depletion. CDV antigen was mainly found in lymphocytes and macrophages of group T dogs, whereas CDV expression was most prominent in dendritic cells of group II animals. In group I dogs, a marked loss of CD3, CD4, CD5, CD8, and IgG expression was noticed, hereby loss of CD4+ cells was mon prominent than depletion of CD8+ cells. In the lymphoid tissues of group II animals, a significant increase in the number of T and B cells was observed compared to group I dogs. The number of CD3+, CD4+, and CD8+ cells in group II dogs was similar to the findings in controls, however, CD5 and IgG expression was mildly reduced in T and B cell areas, respectively. Additionally, in groups I and II dogs, CD3+ and CD5- T cells were detected in T cell areas. Whether this cell population represents a cell type with autoimmune reactive potential remains to be determined. Surprisingly in group II animals, viral antigen was found predominantly in dendritic cells indicating a change in the cell tropism of CDV during chronic infection and a possible mechanism of viral persistence. The two patterns of lymphoid depletions correlated to two different types of canine distemper encephalitis (CDE). Group I dogs displayed acute non-inflammatory CDE, whereas group II does suffered from chronic inflammatory demyelinating CDE, indicating a pathogenic relationship between lymphocytic depletion and inflammatory brain lesions in distemper.
AU  - Wünschmann, A.*
AU  - Kremmer, E.
AU  - Baumgärtner, W.*
C1  - 9503
C2  - 19332
SP  - 83-98
TI  - Phenotypical characterization of T and B cell areas in lymphoid tissues of dogs with spontaneous distemper.
JO  - Vet. Immunol. Immunopathol.
VL  - 73
PB  - Elsevier Scientific Publ. Co.
PY  - 2000
SN  - 0165-2427
ER  - 

TY  - JOUR
AU  - Wünschmann, A.*
AU  - Alldinger, S.*
AU  - Kremmer, E.
AU  - Baumgärtner, W.*
C1  - 20872
C2  - 18926
SP  - 101-116
TI  - Identification of CD4+ and CD8+ T cell subsets and B cells in the brain of dogs with spontaneous acute, subacute-, and chronic-demyelinating distemper encephalitis.
JO  - Vet. Immunol. Immunopathol.
VL  - 67
PY  - 1999
SN  - 0165-2427
ER  - 

TY  - JOUR
AB  - Monoclonal antibodies (mAb) were produced by immunizing BALB/c mice with non-adherent dog lymphocytes. M10 was specific for a subset of dog lymphocytes. M10 belonged to the IgG1 subclass and reacted with 26% of dog peripheral blood lymphocytes, 24% of spleen lymphocytes, 81% of thymus cells, 1.2% of bone marrow cells (5.8% of bone marrow lymphocytes) and 23% of PHA-stimulated lymphocytes. Immunohistology of snap-frozen thymus and spleen showed that the spleen B-cell area stained negative, whereas the spleen T-cell area and the thymus medulla exhibited positive reaction in 20-30%. The thymus cortex was strongly positive. M10 diminished cell lysis by 58% in cell mediated lysis assays (CML). Immunoblot assays revealed that M10 recognized an antigen with a molecular weight of 76 kD under non-reducing and 33 kD under reducing conditions. Finally, M10 bound to a canine CD8α transfected rat T-cell line (NB2). These findings characterize M10 as an antibody directed against the dog CD8 antigen.
AU  - Voß, C.Y.
AU  - Kremmer, E.
AU  - Hoffmann-Fezer, G.
AU  - Schumm, M.A.
AU  - Günther, W.H.H.
AU  - Kolb, H.J.
AU  - Thierfelder, S.S.
C1  - 40285
C2  - 13617
SP  - 311-325
TI  - Identification and characterization of a mouse monoclonal antibody (M10) directed against canine (dog) CD8+ lymphocytes.
JO  - Vet. Immunol. Immunopathol.
VL  - 38
IS  - 3-4
PY  - 1993
SN  - 0165-2427
ER  - 

TY  - JOUR
AB  - Autoimmune hemolytic anemia (AIHA) was detected in pigs affected by spontaneous eperythrozoonosis. Autoantibodies against red cells were found by hemagglutination and hemolysin tests and direct and indirect antiglobulin tests using immunofluorescence. According to these findings and morphological results, the process in porcine eperythrozoonosis is to be classified as acquired autoimmune hemolytic anemia due to "cold" antibodies.
AU  - Hoffmann, R.M.*
AU  - Schmid, D.O.
AU  - Hoffmann-Fezer, G.*
C1  - 41787
C2  - 38571
SP  - 111-119
TI  - Erythrocyte antibodies in porcine eperythrozoonosis.
JO  - Vet. Immunol. Immunopathol.
VL  - 2
IS  - 2
PY  - 1981
SN  - 0165-2427
ER  - 

TY  - JOUR
AB  - Neural lesions of Marek's disease, Marek's disease tumours in the ovary, liver, and kidney, as well as spleen and bursa of Fabricii of chickens bearing Marek's disease tumorous infiltrations, were examined by a new immunohistochemical technique basing upon Sternbergers unlabelled antibody enzyme method which allows the exact localization of lymphoid cells based on their surface antigens. Type C neural lesions contained T-lymphocytes almost exclusively. Type B neural lesions had relatively high proportions of T- and B-lymphocytes, and severe type A neural lesions possessed one part of heavily labelled T-lymphocytes and a number of cells stained weakly by rabbit-antichicken-T-cell-globulin. Tumorous infiltrations had similar characteristics as type A neural lesions. Spleen and interfollicular spaces of bursa of Fabricius were infiltrated by T-lymphocytes.
AU  - Hoffmann-Fezer, G.
AU  - Hoffmann, R.M.*
C1  - 40870
C2  - 38635
SP  - 113-123
TI  - Anatomical distribution of T- and B-lymphocytes in Marek's disease - An immunohistochemical study.
JO  - Vet. Immunol. Immunopathol.
VL  - 1
IS  - 2
PY  - 1980
SN  - 0165-2427
ER  -