TY - JOUR AB - In the last years, progestins have raised special concerns for their documented negative effects on aquatic species, yet little is known about their environmental levels in surface waters and bioaccumulation in the trophic web. This study aimed to 1) adapt an extraction method for quantifying progestins in freeze-dried matrices, 2) validate the analytical procedure for three matrices: bivalve, polychaete, and crustacean, and 3) characterize levels of the four most prescribed synthetic progestins in key species across three Portuguese estuaries. Through the validated method, progestins were only quantifiable for the crustacean. Values were generally low, peaking with drospirenone values in Ria de Aveiro (1.33 ± 0.26 ng/g ww) and Tagus estuary (1.42 ± 0.55 ng/g ww), while Ria Formosa exhibited the lowest progestin concentrations (< 1 ng/g ww). This study enabled the development of a precise extraction and analytical method for quantifying steroid hormones in three distinct biological matrices. AU - Amorim, V.E.* AU - Morais, H.* AU - Ferreira, A.C.S.* AU - Pardal, M.A.* AU - Cruzeiro, C. AU - Cardoso, P.G.* C1 - 69024 C2 - 53809 CY - The Boulevard, Langford Lane, Kidlington, Oxford Ox5 1gb, England TI - Application of a robust analytical method for quantifying progestins in environmental samples from three Portuguese Estuaries. JO - Mar. Pollut. Bull. VL - 199 PB - Pergamon-elsevier Science Ltd PY - 2023 SN - 0025-326X ER - TY - JOUR AB - Coastal areas are affected by multiple stressors like climate change and endocrine disruptors (EDCs). In the laboratory, we investigated the combined effects of increased temperature and EDCs (drospirenone and mercury) on the fitness and gonads' maturation dynamics of the marine gastropod Nucella lapillus for 21 days. Survival was negatively affected by all the stressors alone, while, in combination, a synergistic negative effect was observed. Both chemicals, as single factors, did not cause any effect on the maturation stage of ovaries and testis. However, in the presence of a higher temperature, it was clear a delay in the maturation stage of the ovaries, but not in the testis, suggesting a higher negative impact of the stressors in females than in males. In summary, drospirenone caused a low negative impact in aquatic species, like gastropods, but in combination with other EDCs and/or increased temperature can be a matter of concern. AU - Morais, H.* AU - Arenas, F.* AU - Cruzeiro, C. AU - Galante-Oliveira, S.* AU - Cardoso, P.G.* C1 - 67665 C2 - 53972 CY - The Boulevard, Langford Lane, Kidlington, Oxford Ox5 1gb, England TI - Combined effects of climate change and environmentally relevant mixtures of endocrine disrupting compounds on the fitness and gonads' maturation dynamics of Nucella lapillus (Gastropoda). JO - Mar. Pollut. Bull. VL - 190 PB - Pergamon-elsevier Science Ltd PY - 2023 SN - 0025-326X ER - TY - JOUR AB - Synthetic progestins are micropollutants of special concern, due to their growing use in human and veterinary therapies and their risks to aquatic life. Currently, there is a lack of environmental information on these compounds, worldwide. The main objective of this work was to characterize the levels of the most consumed progestins in Portugal. For that, Ria de Aveiro, Tagus estuary and Ria Formosa were sampled in a temporal perspective to evaluate levels of drospirenone (DRO), desogestrel (DSG), gestodene (GST) and levonorgestrel (LNG). Drospirenone and desogestrel were the most abundant progestins. In the North of Portugal, DSG was the most abundant (Aveiro: 193.9 ng L-1 in summer), while DRO was more representative in the South (Tagus: 178.9 ng L-1; Formosa: 125.7 ng L-1) and also in summer. These spatial differences can be associated with the hydrodynamics of each estuarine system as well as the distinct population and tourist levels associated with each site. AU - Morais, H.* AU - Cruzeiro, C. AU - Pardal, M.A.* AU - Cardoso, P.G.* C1 - 68002 C2 - 54480 CY - The Boulevard, Langford Lane, Kidlington, Oxford Ox5 1gb, England TI - Baseline progestins characterization in surface waters of three main Portuguese estuaries. JO - Mar. Pollut. Bull. VL - 194 IS - Pt B PB - Pergamon-elsevier Science Ltd PY - 2023 SN - 0025-326X ER - TY - JOUR AB - Polycyclic aromatic hydrocarbons (PAH), as a part of dissolved organic matter (DOM), are environmental pollutants of the marine compartment. This study investigates the origin of PAH, which is supposed to derive mainly from anthropogenic activities, and their alteration along the salinity gradient of the Baltic Sea. Pyrolysis in combination with gas chromatography and two mass selective detectors in one measurement cycle are utilized as a tool for an efficient trace analysis of such complex samples, by which it is possible to detect degradation products of high molecular structures. Along the north-south transect of the Baltic Sea a slightly rising trend for PAH is visible. Their concentration profiles correspond to the ship traffic as a known anthropogenic source, underlined by the value of special isomer ratios such as phenanthrene and anthracene (0.31-0.45) or pyrene and fluoranthene (0.44-0.53). The detection of naphthalene and the distribution of its alkylated representatives support this statement. AU - Otto, S.* AU - Streibel, T. AU - Erdmann, S.* AU - Klingbeil, S.* AU - Schulz-Bull, D.* AU - Zimmermann, R. C1 - 46588 C2 - 37695 SP - 35-42 TI - Pyrolysis-gas chromatography-mass spectrometry with electron-ionization or resonance-enhanced-multi-photon-ionization for characterization of polycyclic aromatic hydrocarbons in the Baltic Sea. JO - Mar. Pollut. Bull. VL - 99 IS - 1-2 PY - 2015 SN - 0025-326X ER - TY - JOUR AB - Water concentrations of polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs) and organochlorine pesticides (OCPs) were estimated from semipermeable membrane devices (SPMDs) and from sediment pollutant concentrations. SPMDs were deployed in the Istanbul Strait and Marmara Sea and retrieved after 7 and 21 days. Performance reference compounds (PRCs) were used to determine the site-specific sampling rates of the compounds. Water concentrations (C-w) of the analyzed compounds estimated by using two different calculation methods for SPMDs were found similar. C-w of total PAHs estimated from SPMDs (Cw-spmd) were found between 13 and 79 ng L-1 and between 7.0 and 68 ng L-1 for 7 and 21 days of deployments respectively. Water concentrations of PCBs using sediment data was found as between 0.001 and 11.0 ng L-1. The highest value of Cw-spmd for two deployments were 2.8 ng L-1 for OCPs. C-w estimated from sediment concentrations were generally higher than those estimated from SPMDs. (C) 2013 Elsevier Ltd. All rights reserved. AU - Karacik, B.* AU - Okay, O.S.* AU - Henkelmann, B. AU - Pfister, G. AU - Schramm, K.-W. C1 - 25927 C2 - 31983 SP - 258-265 TI - Water concentrations of PAH, PCB and OCP by using semipermeable membrane devices and sediments. JO - Mar. Pollut. Bull. VL - 70 IS - 1-2 PB - Pergamon-Elsevier Science PY - 2013 SN - 0025-326X ER - TY - JOUR AB - Polycyclic aromatic hydrocarbons (PAHs) are important environmental pollutants due to their persistence and bioaccumulation potential both in organisms and in sediments. In this study, bioaccumulation and depuration experiments were performed employing local Mediterranean mussel species, Mytilus galloprovincialis, with two biomarkers: filtration rate and lysosomal stability (neutral red retention) assay. Benzo(a)anthracene (BaA) was chosen as the model PAH compound due to its common presence in several matrices in the marine environment. Bioconcentration Factors (BCFs) for the mussels exposed to different BaA concentrations were calculated from both kinetic rate constants and from the experimental data and found between 27-3184 and 16-2745, respectively. Experimental QSAR values were found comparable to the previous QSAR models created for BaA. The effect of BaA exposure on the mussels showed a more clear dose-response relationship according to the results of lysosomal stability compared to the filtration rate. AU - Yakan, S.D.* AU - Henkelmann, B. AU - Schramm, K.-W. AU - Okay, O.S.* C1 - 6907 C2 - 29412 SP - 471-476 TI - Bioaccumulation depuration kinetics and effects of benzo(a)anthracene on Mytilus galloprovincialis. JO - Mar. Pollut. Bull. VL - 63 IS - 5-12 PB - Elsevier PY - 2011 SN - 0025-326X ER - TY - JOUR AB - For the first time the concentrations of copper, zinc, mercury, cadmium and lead in hair samples of the Mediterranean Monk Seal, one of the world's rarest mammals, were determined. The hair samples were collected in the absence of the animals from six caves located in the Ionian Sea, Greece. Our data suggest the importance of hair analysis as a useful indicator for heavy metals in these rare animals. AU - Yediler, A. AU - Panou, A. AU - Schramel, P. C1 - 40422 C2 - 40039 SP - 156-159 TI - Heavy metals in hair samples of the mediterranean monk seal (Monachus monachus). JO - Mar. Pollut. Bull. VL - 26 IS - 3 PY - 1993 SN - 0025-326X ER - TY - JOUR AU - Yediler, Y. AU - Panou, A. AU - Schramel, P. C1 - 19264 C2 - 12336 SP - 156-159 TI - Heavy Metals in Hair Samples of the Mediterranean Monk Seal (Monachus monachus). JO - Mar. Pollut. Bull. VL - 26 PY - 1993 SN - 0025-326X ER -