TY  - JOUR
AB  - Aims: To develop an estrone-targeted d-alpha-tocopherol polyethylene glycol 1000 succinate (TPGS)-based liposomal system for enhanced intracellular delivery of doxorubicin (DOX). Materials & methods: Zetasizer, transmission electron microscopy, energy dispersive x-ray, Fourier-transform infrared spectroscopy, differential scanning calorimetry, x-ray diffraction, confocal laser scanning microscopy and FACS analysis were used for formulation characterization and evaluation. Results: The DOX-LIPO-TPGS and DOX-LIPO-TPGS-estrone formulations had vesicle sizes (117.6 ± 3.51; 144 ± 5.00 nm), zeta potential (-36.4 ± 0.75; -35.8 ± 0.76), polydispersity index (0.123 ± 0.005; 0.169 ± 0.005) and percent entrapment efficiency (73.56 ± 3.55; 77.16 ± 3.83%) with improved cytotoxicity and cellular uptake, confirming the targeted potential of the developed formulations. Conclusion: The results suggest that the developed liposomal formulation with desired characteristics is potentially capable of nonimmunogenic, site-specific drug delivery to targeted cancer sites and reduced DOX-associated cardiac toxicity.
AU  - Kurmi, B.D.*
AU  - Patel, P.*
AU  - Paliwal, R.*
AU  - Kumar, P.
AU  - Paliwal, S.R.*
C1  - 68622
C2  - 54791
CY  - Unitec House, 3rd Floor, 2 Albert Place, Finchley Central, London, N3 1qb, England
SP  - 1261-1279
TI  - Multifunctional nanotherapeutics for intracellular trafficking of doxorubicin against breast cancer.
JO  - Nanomed.
VL  - 18
IS  - 19
PB  - Future Medicine Ltd
PY  - 2023
SN  - 1743-5889
ER  - 

TY  - JOUR
AB  - The potential of poly(lactic-co-glycolic acid) (PLGA) to design nanoparticles (NPs) and target the central nervous system remains to be exploited. In the current study we designed fluorescent 70-nm PLGA NPs, loaded with bulky fluorophores, thereby making them significantly brighter than quantum dots in single-particle fluorescence measurements. The high brightness of NPs enabled their visualization by intravital real-time 2-photon microscopy. Subsequently, we found that PLGA NPs coated with pluronic F-68 circulated in the blood substantially longer than uncoated NPs and were taken up by cerebro-vascular endothelial cells. Additionally, confocal microscopy revealed that coated PLGA NPs were present in late endothelial endosomes of cerebral vessels within 1hour after systemic injection and were more readily taken up by endothelial cells in peripheral organs. The combination of ultra-bright NPs and in vivo imaging may thus represent a promising approach to reduce the gap between development and clinical application of nanoparticle-based drug carriers.
AU  - Khalin, I.*
AU  - Severi, C.*
AU  - Heimburger, D.*
AU  - Wehn, A.*
AU  - Hellal, F.
AU  - Reisch, A.*
AU  - Klymchenko, A.S.*
AU  - Plesnila, N.*
C1  - 63907
C2  - 51983
TI  - Dynamic tracing using ultra-bright labelling and multi-photon microscopy identifies endothelial uptake of poloxamer 188 coated poly(lactic-co-glycolic acid) nano-carriers in vivo.
JO  - Nanomed.
VL  - 40
PY  - 2022
SN  - 1743-5889
ER  - 

TY  - JOUR
AB  - Lung cancer is by far the most common cause of cancer-related deaths in the world. Nanoparticle-based therapies enable targeted drug delivery for lung cancer treatment with increased therapeutic efficiency and reduced systemic toxicity. At the same time, nanomedicine has the potential for multimodal treatment of lung cancer that may involve 'all-in-one' targeting of several tumor-associated cell types in a timely and spatially controlled manner. Therapeutic approaches, however, are hampered by a translational gap between basic scientists, clinicians and pharma industry due to suboptimal animal models and difficulties in scale-up production of nanoagents. This calls for a disease-centered approach with interdisciplinary basic and clinical research teams with the support of pharma industries.
AU  - Bölükbas, D.A.
AU  - Meiners, S.
C1  - 47181
C2  - 39141
SP  - 3203-3212
TI  - Lung cancer nanomedicine: Potentials and pitfalls.
JO  - Nanomed.
VL  - 10
IS  - 21
PY  - 2015
SN  - 1743-5889
ER  - 

TY  - JOUR
AB  - AIM: To investigate the influence of gold nanoparticle geometry on the biochemical response of Calu-3 epithelial cells. MATERIALS & METHODS: Spherical, triangular and hexagonal gold nanoparticles (GNPs) were used. The GNP-cell interaction was assessed via atomic absorption spectroscopy (AAS) and transmission electron microscopy (TEM). The biochemical impact of GNPs was determined over 72 h at (0.0001-1 mg/ml). RESULTS: At 1 mg/ml, hexagonal GNPs reduced Calu-3 viability below 60%, showed increased reactive oxygen species production and higher expression of proapoptotic markers. A cell mass burden of 1:2:12 as well as number of GNPs per cell (2:1:3) was observed for spherical:triangular:hexagonal GNPs. CONCLUSION: These findings do not suggest a direct shape-toxicity effect. However, do highlight the contribution of shape towards the GNP-cell interaction which impacts upon their intracellular number, mass and volume dose. KEYWORDS: CD95 (APO-1/Fas); Cathepsin-B; biocompatibility; caspases; cell death; gold nanoparticles; lung epithelial cells; nanoparticle shape; nanotoxicology; reactive oxygen species  
AU  - Tian, F.
AU  - Clift, M.J.*
AU  - Casey, A.*
AU  - del Pino, P.*
AU  - Pelaz, B.*
AU  - Conde, J.*
AU  - Byrne, H.J.*
AU  - Rothen-Rutishauser, B.*
AU  - Estrada, G.*
AU  - de la Fuente, J.M.*
AU  - Stöger, T.
C1  - 46827
C2  - 37875
SP  - 2643-2657
TI  - Investigating the role of shape on the biological impact of gold nanoparticles in vitro.
JO  - Nanomed.
VL  - 10
IS  - 17
PY  - 2015
SN  - 1743-5889
ER  - 

TY  - JOUR
AB  - Aim: To investigate the relationship of alveolar macrophages and inhaled nanoparticles in the lung. Materials & methods: Rats were exposed by inhalation to 14 nm gold nanoparticles for 6 hours, and ultramicroscopic observation on the frequency and localization of gold nanoparticles within lavaged macrophages was performed up to 7 days. Results & discussion: The majority of macrophages examined on day 0 (94%) contained internalized gold nanoparticles, and the percentage decreased to 59% on day 7. Gold nanoparticles were exclusively found within cytoplasmic vesicles. On day 0, most gold nanoparticles appeared to be individual or slightly agglomerated, and they were frequently agglomerated on day 7. Conclusion: Alveolar macrophages efficiently internalized nanoparticles by endocytosis, and re-arrangements of vesicles and of nanoparticles in the vesicles of macrophages occurred.
AU  - Takenaka, S.
AU  - Möller, W.
AU  - Semmler-Behnke, M.
AU  - Karg, E.W.
AU  - Wenk, A.
AU  - Schmid, O.
AU  - Stöger, T.
AU  - Jennen, L.
AU  - Aichler, M.
AU  - Walch, A.K.
AU  - Pokhrel, S.*
AU  - Mädler, L.*
AU  - Eickelberg, O.
AU  - Kreyling, W.G.
C1  - 7202
C2  - 29547
SP  - 855-865
TI  - Efficient internalization and intracellular translocation of inhaled gold nanoparticles in rat alveolar macrophages.
JO  - Nanomed.
VL  - 7
IS  - 6
PB  - Future Medicine
PY  - 2012
SN  - 1743-5889
ER  -