TY  - JOUR
AB  - Unlike traditional optical methods, optoacoustic imaging is less sensitive to scattering of ballistic photons, so it is capable of high-resolution interrogation at a greater depth. By integrating video-rate visualization with multiplexing and sensing a range of endogenous and exogenous chromophores, optoacoustic imaging has matured into a versatile noninvasive investigation modality with rapidly expanding use in biomedical research. We review the principal features of the technology and discuss recent advances it has enabled in structural, functional, and molecular neuroimaging in small-animal models. In extending the boundaries of noninvasive observation beyond the reach of customary photonic methods, the latest developments in optoacoustics have substantially advanced neuroimaging inquiry, with promising implications for basic and translational studies.
AU  - Ovsepian, S.V.
AU  - Olefir, I.
AU  - Ntziachristos, V.
C1  - 57284
C2  - 47708
CY  - 84 Theobalds Rd, London Wc1x 8rr, England
SP  - 1315-1326
TI  - Advances in optoacoustic neurotomography of animal models.
JO  - Trends Biotechnol.
VL  - 37
IS  - 12
PB  - Elsevier Science London
PY  - 2019
SN  - 0167-7799
ER  - 

TY  - JOUR
AB  - One in six couples of reproductive age worldwide are affected at least once by some form of infertility. In vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) are widely-available assisted reproductive technologies (ART). The identification and isolation of the most-motile sperm with DNA integrity are essential to IVF and ICSI, ultimately affecting treatment consequences and the health of offspring. Recently, microfluidic technologies been developed to sort sperm according to sperm morphology, motility, DNA integrity, and functionality for IVF techniques. There have also been emerging applications in wildlife conservation, high-throughput single-sperm genomics, sperm-driven robotics, and in-home fertility testing. We review a broad range of studies applying the principles of microfluidics to sperm research.
AU  - Knowlton, S.M.*
AU  - Sadasivam, M.
AU  - Tasoglu, S.*
C1  - 43952
C2  - 36681
CY  - London
SP  - 221-229
TI  - Microfluidics for sperm research.
JO  - Trends Biotechnol.
VL  - 33
IS  - 4
PB  - Elsevier Science London
PY  - 2015
SN  - 0167-7799
ER  - 

TY  - JOUR
AB  - The past decade marked an optical revolution in biology: an unprecedented number of optical techniques were developed and adopted for biological exploration, demonstrating increasing interest in optical imaging and in vivo interrogations. Optical methods have become faster and have reached nanoscale resolution, and are now complemented by optoacoustic (photoacoustic) methods capable of imaging whole specimens in vivo. Never before were so many optical imaging barriers broken in such a short time-frame: with new approaches to optical microscopy and mesoscopy came an increased ability to image biology at unprecedented speed, resolution, and depth. This review covers the most relevant techniques for imaging in developmental biology, and offers an outlook on the next steps for these technologies and their applications.
AU  - Ripoll, J.*
AU  - Koberstein-Schwarz, B.
AU  - Ntziachristos, V.
C1  - 47158
C2  - 39123
SP  - 679-691
TI  - Unleashing optics and optoacoustics for developmental biology.
JO  - Trends Biotechnol.
VL  - 33
IS  - 11
PY  - 2015
SN  - 0167-7799
ER  - 

TY  - JOUR
AU  - Werner, T.
C1  - 9834
C2  - 20965
SP  - 9-13
TI  - Promoters can contribute to the elucidation of protein function.
JO  - Trends Biotechnol.
VL  - 21
PY  - 2003
SN  - 0167-7799
ER  - 

TY  - JOUR
AB  - The high specificity and sensitivity of fluorescence techniques have made them important analytical tools in medicine and biotechnology. Besides monitoring and quantitative detection of biomolecules these methods can be used for controlling bacterial activities or for measuring physiological states of cells or tissues. Three topics of importance in biotechnology - immunoassays, photosynthesis and fermentation - are treated in detail.
AU  - Schneckenburger, H.
AU  - Reuter, B.W.
AU  - Schoberth, S.M.
C1  - 41790
C2  - 38220
SP  - 257-261
TI  - Fluorescence techniques in biotechnology.
JO  - Trends Biotechnol.
VL  - 3
IS  - 10
PY  - 1985
SN  - 0167-7799
ER  -