TY  - JOUR
AB  - OBJECTIVE: The influence of different exposures to PM2.5 (particulate matter with an aerodynamic diameter below 2.5 µm) on the concentrations of biomarkers of exposure and oxidative stress should be investigated. For this purpose urine samples from 9 individuals travelling from Germany to China were collected and analysed. MATERIALS: Robust LC and LC-MS/MS methods were established for the determination of biomarkers including 8-hydroxy-2'deoxyguanosine, malondialdehyde, F2α-isoprostanes, and hydroxylated polycyclic aromatic hydrocarbons. As a pilot study, 9 volunteers travelled from Germany (mean daily concentration of PM2.5: 21 µg/m(3)) to China (mean daily concentration of PM2.5: 108 µg/m(3)). Urine samples were collected before and after the trip. RESULTS: In samples collected after return to Germany, the median concentrations of oxidative stress biomarkers were observed to be higher than in samples collected before leaving Germany. Decreasing trends were observed in the sequences of samples collected after return in the following weeks. Correlations were found between exposure and oxidative stress biomarkers. CONCLUSION: Travellers are ideal models for PM pollution induced acute health effects study. Exposure to PM pollution can cause oxidative stress and damage.
AU  - Wu, X.
AU  - Lintelmann, J.
AU  - Klingbeil, S.*
AU  - Li, J.*
AU  - Wang, H.*
AU  - Kuhn, E.
AU  - Ritter, S.
AU  - Zimmermann, R.
C1  - 50786
C2  - 42698
CY  - Abingdon
SP  - 1-27
TI  - Determination of air pollution-related biomarkers of exposure in urine of travellers between Germany and China using liquid chromatographic and liquid chromatographic-mass spectrometric methods: A pilot study.
JO  - Biomarkers
VL  - 22
IS  - 6
PB  - Taylor & Francis Ltd
PY  - 2017
SN  - 1354-750X
ER  - 

TY  - JOUR
AB  - Blood levels of cyto- and chemokines might reflect immune deregulations which might be related to lymphomagenesis. Potential utility of stored blood samples of a prospective cohort was evaluated by the effect of different blood sample types and freeze-thaw cycles on analyte levels. Bead-based immunoassays were performed on two fresh samples (serum, citrate and heparin plasma) of 10 asymptomatic adults collected 14 days apart and on aliquots of the first samples which were put through one to three freeze-thaw cycles to measure 11 cytokines, four chemokines and two adhesion molecules. Median coefficients of variation (CVs) of the measured analytes were 20%, 24% and 32% in serum, citrate and heparin plasma, respectively. Strong correlations (rank correlation coefficient 0.74-0.98) were observed between sample types, although small differences in analyte levels were observed for most analytes. Freeze-thaw cycles did not markedly change analyte levels. Our study supports the use of this assay among asymptomatic subjects in epidemiological studies.
AU  - Hosnijeh, F.S.*
AU  - Krop, E.J.M.*
AU  - Portengen, L.*
AU  - Rabkin, C.S.*
AU  - Linseisen, J.
AU  - Vineis, P.*
AU  - Vermeulen, R.*
C1  - 2021
C2  - 27390
SP  - 140-148
TI  - Stability and reproducibility of simultaneously detected plasma and serum cytokine levels in asymptomatic subjects.
JO  - Biomarkers
VL  - 15
IS  - 2
PB  - Taylor & Francis Ltd.
PY  - 2010
SN  - 1354-750X
ER  - 

TY  - JOUR
AB  - Both epidemiological and toxicological studies indicate that inhalation and subsequent deposition of air borne particles into the lungs have adverse health effects. Recently, the ultrafine particle (UfP) fraction (diameter < 100nm) has received particular attention, as their small size may lead to more toxic proper ties. In this study we summarize the current knowledge on the dosimetry of inhaled particles (including UfPs) with a focus on recent data on translocation of UfPs into secondary target organs (such as brain and heart) suggesting that the lifetime dose of ambient UfPs in secondary target organs is about 1011 particles. Furthermore, we highlight the main pathways of particle induced toxicity and the reasons for the potentially higher toxicity of UfPs. Finally, we discuss recent evidence indicating that (BET) surface area is the single most relevant dose metric for the toxicity of UfPs, which has important implications for regulatory measures on the toxicity of ambient and engineered particles.
AU  - Schmid, O.
AU  - Möller, W.
AU  - Semmler-Behnke, M.
AU  - Ferron, G.A.
AU  - Karg, E.W.
AU  - Lipka, J.
AU  - Schulz, S.
AU  - Kreyling, W.G.
AU  - Stöger, T.
C1  - 896
C2  - 26412
CY  - Abingdon
SP  - 67-73
TI  - Dosimetry and toxicology of inhaled ultrafine particles.
JO  - Biomarkers
VL  - 14
IS  - SUPPL.1
PB  - Taylor & Francis
PY  - 2009
SN  - 1354-750X
ER  - 

TY  - JOUR
AB  - Several epidemiological studies have shown that in the human population ambient particulate matter (PM) is associated with adverse health effects. Little is known, however, about the relative effects of aerosol constituents. Since 2002, diurnal samples of ambient PM2.5 were analysed by automated methods for the quantification of particle-associated organic compounds (POC). Data on chemical composition have been investigated in epidemiological and biological effect studies. As a result of these studies, the associations found between PAH concentration and symptoms of myocardial infarction survivors suggest a major influence of combustion sources on cardiovascular health effects. The correlations found between formation of reactive oxygen species and the presence of specific organic compounds suggests an important influence of biomass combustion particles in PM2.5-associated oxidative stress.
AU  - Schnelle-Kreis, J.
AU  - Küpper, U.
AU  - Sklorz, M.*
AU  - Cyrys, J.
AU  - Briedé, J.J.*
AU  - Peters, A.
AU  - Zimmermann, R.
C1  - 895
C2  - 27110
CY  - Abingdon
SP  - 39-44
TI  - Daily measurement of organic compounds in ambient particulate matter in Augsburg, Germany: New aspects on aerosol sources and aerosol related health effects.
JO  - Biomarkers
VL  - 14
IS  - SUPPL.1
PB  - Taylor & Francis
PY  - 2009
SN  - 1354-750X
ER  -