TY - JOUR AU - Ertl, M.* AU - Raasch, N.* AU - Hammel, G. AU - Zeiser, K. AU - Lang, C.* C1 - 55582 C2 - 46515 CY - Ste 800, 230 Park Ave, New York, Ny 10169 Usa SP - 1344 TI - Transtemporal investigation of brain parenchyma elasticity using 2-D shear wave elastography: Validation appreciated! JO - Ultrasound Med. Biol. VL - 45 IS - 5 PB - Elsevier Science Inc PY - 2019 SN - 0301-5629 ER - TY - JOUR AB - Human skull poses a significant barrier for the propagation of ultrasound waves. Development of methods enabling more efficient ultrasound transmission into and from the brain is therefore critical for the advancement of ultrasound-mediated transcranial imaging or actuation techniques. We report on the first observation of guided acoustic waves in the near field of an ex vivo human skull specimen in the frequency range between 0.2 and 1.5 MHz. In contrast to what was previously observed for guided wave propagation in thin rodent skulls, the guided wave observed in a higher-frequency regime corresponds to a quasi-Rayleigh wave, confined mostly to the cortical bone layer. The newly discovered near-field properties of the human skull are expected to facilitate the development of more efficient diagnostic and therapeutic techniques based on transcranial ultrasound. (C) 2018 World Federation for Ultrasound in Medicine & Biology. All rights reserved. AU - Estrada, H. AU - Gottschalk, S. AU - Reiss, M. AU - Neuschmelting, V. AU - Goldbrunner, R.* AU - Razansky, D. C1 - 54116 C2 - 45357 CY - 360 Park Ave South, New York, Ny 10010-1710 Usa SP - 2388-2392 TI - Observation of guided acoustic waves in a human skull. JO - Ultrasound Med. Biol. VL - 44 IS - 11 PB - Elsevier Science Inc PY - 2018 SN - 0301-5629 ER - TY - JOUR AB - The goal of our research was to assess the possibility of reliable investigation of brain tissue stiffness using ultrasonographic brain parenchyma elastography with an intact temporal bone. We enrolled 108 patients after exclusion of intracranial pathology or healthy volunteers. All patients were subdivided by age into groups: 20-40, 40-60 and >60 y. For statistical analysis, the χ(2) test and t-test were used. The mean values, regardless of age and other parameters, were 3.34 kPa (SD = 0.59) on the left side and 3.33 kPa (SD = 0.58) on the right side. We found no correlation between the values, body mass index (r = 0.07, p = 0.48) and sex (t = -0.11, p = 0.91), but we observed a highly significant correlation between the values and age (r = 0.43, p <0.0001). We found ultrasonographic brain parenchyma elastography to be a valid, reproducible and investigator-independent method that reliably determines brain parenchyma stiffness. Normal values should serve as a reference for studies on various intracranial lesions. AU - Ertl, M.* AU - Raasch, N.* AU - Hammel, G. AU - Zeiser, K. AU - Lang, C.* C1 - 52055 C2 - 43695 CY - New York SP - 78-84 TI - Transtemporal investigation of brain parenchyma elasticity using 2-D shear wave elastography: Definition of age-matched normal values. JO - Ultrasound Med. Biol. VL - 44 IS - 1 PB - Elsevier Science Inc PY - 2017 SN - 0301-5629 ER - TY - JOUR AB - We present a novel approach to transcranial B-mode sonography for Parkinson's disease (PD) diagnosis by using 3-D ultrasound (3-DUS). We reconstructed bilateral 3-DUS volumes of the midbrain and substantia nigra echogenicities (SNE) and report results of a more objective abnormality detection in (PD). For classification, we analyzed volumetric measurements of midbrain and SNE in subjects with PD and healthy controls (HC). After blinded segmentation of these structures in 22/23 subjects (11 PD, 11 HC) and by two observers with varying prior experience in this technique, the classification algorithm yielded up to 91% sensitivity and 64% specificity using the larger volume of both SNE as a single-dimensional features and up to 90.9% sensitivity and 72.7% specificity using a multidimensional feature set with midbrain and both SNE volumes. This pilot study indicates that our TC-3-D-US approach is technically feasible and less dependent on the investigator's experience and good bone windows. Our pilot study yielded a fairly high sensitivity and specificity in differentiating between subjects with PD and HC. AU - Plate, A.* AU - Ahmadi, S.A.* AU - Pauly, O. AU - Klein, T.* AU - Navab, N.* AU - Botzel, K.* C1 - 11512 C2 - 30728 SP - 2041-2050 TI - Three-dimensional sonographic examination of the midbrain for computer-aided diagnosis of movement disorders. JO - Ultrasound Med. Biol. VL - 38 IS - 12 PB - Elsevier PY - 2012 SN - 0301-5629 ER - TY - JOUR AB - Cell membrane damage by ultrasound was studied in human nucleated cells in vitro at various concentrations. Suspensions of human blood cells, cells of a human leukemic cell line (Reh) and mixtures of nucleated cells with erythrocytes were exposed to continuous ultrasound of 782 kHz at a SPTA intensity of 15 W/cm 2. The surviving nucleated cells without membrane damage were counted on the basis of exclusion of ethidiumbromide using flow cytometer. At high cell concentrations as present in whole blood we observed no cell death, whereas below 5 x 10 7 cells/ml most of the granulocytes, stimulated lymphocytes and Reh cells were damaged. The concentration threshold below which cells were damaged seemed not to depend on the size of the cells, rather on the concentration of particles in the suspension. | Cell membrane damage by ultrasound was studied in human nucleated cells in vitro at various concentrations. Suspensions of human blood cells, cells of a human leukemic cell line (Reh) and mixtures of nucleated cells with erythrocytes were exposed to continuous ultrasound of 782 kHz at a SPTA intensity of 15 W/cm**2. The surviving nucleated cells without membrane damage were counted on the basis of exclusion of ethidiumbromide using a flow cytometer. At high cell concentrations as present in whole blood, we observed no cell death, whereas below 5 multiplied by 10**7 cells/ml most of the granulocytes, stimulated lymphocytes and Reh cells were damaged. The concentration threshold below which cells were damaged seemed not to depend on the size of the cells, rather on the concentration of particles in the suspension. AU - Ellwart, J.W. AU - Brettel, H. AU - Kober, L.O.M. C1 - 42366 C2 - 36186 SP - 43-50 TI - Cell membrane damage by ultrasound at different cell concentrations. JO - Ultrasound Med. Biol. VL - 14 IS - 1 PY - 1988 SN - 0301-5629 ER -