TY  - JOUR
AB  - Industrial biotechnology offers a potential ecological solution for PET 
      recycling under relatively mild reaction conditions via enzymatic 
      degradation, particularly using the leaf branch compost cutinase (LCC) 
      quadruple mutant ICCG. To improve the efficient downstream processing of
       this biocatalyst after heterologous gene expression with a suitable 
      production host, protein crystallization can serve as an effective 
      purification/capture step. Enhancing protein crystallization was 
      achieved in recent studies by introducing electrostatic (and aromatic) 
      interactions in two homologous alcohol dehydrogenases (Lb/LkADH) and an ene reductase (NspER1-L1,5) produced with Escherichia coli.
       In this study, ICCG, which is difficult to crystallize, was utilized 
      for the application of crystal contact engineering strategies, resulting
       in ICCG mutant L50Y (ICCGY). Previously focused on the Lys-Glu 
      interaction for the introduction of electrostatic interactions at 
      crystal contacts, the applicability of the engineering strategy was 
      extended here to an Arg-Glu interaction to increase crystallizability, 
      as shown for ICCGY T110E. Furthermore, the rationale of the engineering 
      approach is demonstrated by introducing Lys and Glu at non-crystal 
      contacts or sites without potential interaction partners as negative 
      controls. These resulting mutants crystallized comparably but not 
      superior to the wild-type protein. As demonstrated by this study, 
      crystal contact engineering emerges as a promising approach for 
      rationally enhancing protein crystallization. This advancement could 
      significantly streamline biotechnological downstream processing, 
      offering a more efficient pathway for research and industry.
AU  - Walla, B.*
AU  - Dietrich, A.*
AU  - Brames, E.*
AU  - Bischoff, D.*
AU  - Fritzsche, S.*
AU  - Castiglione, K.*
AU  - Janowski, R.
AU  - Niessing, D.
AU  - Weuster‐Botz, D.*
C1  - 74832
C2  - 57619
CY  - Mdpi Ag, Grosspeteranlage 5, Ch-4052 Basel, Switzerland
SP  - 561 - 561
TI  - Application of a rational crystal contact engineering strategy on a poly(ethylene terephthalate)-degrading cutinase.
JO  - Bioengineering
VL  - 12
IS  - 6
PB  - Mdpi
PY  - 2025
SN  - 2306-5354
ER  - 

TY  - JOUR
AU  - Prell, H.H.
C1  - 17282
C2  - 10324
TI  - Molekulare Genetik des Kartoffel-Pathogens Phytophtora infestans.
JO  - Bioengineering
PY  - 1988
SN  - 2306-5354
ER  -