TY  - JOUR
AB  - BACKGROUND: Polymorphic light eruption (PLE) is the most frequent photodermatosis in Europe with an estimated prevalence of 10 to 20%, particularly in temperate climates. Itching or burning lesions appear only in sun-exposed areas, predominantly on the chest, the arms and forearms within a few hours following exposure. The disease's cause is still unknown, yet studies have suggested that skin microbial elements may play a role in its pathogenesis. OBJECTIVES: We investigated in this cohort the skin microbiome of PLE patients upon exposure to ultraviolet radiation (UVR), to assess its role in the onset of PLE lesions. METHODS: Forty-one skin swabs have been collected from eleven PLE patients at baseline and following a three-day exposure to UVR and from healthy controls. The collected swabs were analyzed for their microbial composition using a 16S amplicon sequencing approach. RESULTS: The PLE skin showed a dysbalanced microbiome, already at baseline, with significantly reduced microbial diversity and noticeable colonization by bacterial pathogens as Staphylococcus aureus. Upon UVR exposure, the PLE microbiome exhibited a further loss of diversity and decline of beneficial skin commensals. In line with this, we observed that UVR exerted strong antimicrobial effects in vitro against representative skin residents. CONCLUSIONS: Taken together, UVR can lead to profound skin microbiome changes, allowing the proliferation of dysbiotic members that can release a variety of elements able to trigger PLE lesions. This is the first study investigating the cutaneous microbiome changes in PLE patients upon UVR, offering new insights into disease pathogenesis, so far unexplored.
AU  - Amar, Y.*
AU  - Niedermeier, S.*
AU  - Silva, R.*
AU  - Kublik, S.
AU  - Schloter, M.
AU  - Biedermann, T.*
AU  - Köberle, M.*
AU  - Eberlein, B.*
C1  - 72478
C2  - 56610
CY  - Great Clarendon St, Oxford Ox2 6dp, England
SP  - 684-696
TI  - Skin microbiome dynamics in patients with polymorphic light eruption in response to UV radiations.
JO  - Br. J. Dermatol.
VL  - 192
IS  - 4
PB  - Oxford Univ Press
PY  - 2024
SN  - 0007-0963
ER  - 

TY  - JOUR
AU  - Stölzl, D.*
AU  - Sander, N.*
AU  - Heratizadeh, A.*
AU  - Haufe, E.*
AU  - Harder, I.*
AU  - Abraham, S.*
AU  - Heinrich, L.*
AU  - Kleinheinz, A.*
AU  - Wollenberg, A.*
AU  - Weisshaar, E.*
AU  - Schäkel, K.*
AU  - Ertner, K.*
AU  - Wiemers, F.*
AU  - Wildberger, J.*
AU  - Worm, M.*
AU  - von Kiedrowski, R.*
AU  - Effendy, I.*
AU  - Asmussen, A.*
AU  - Augustin, M.*
AU  - Pawlak, M.*
AU  - Sticherling, M.*
AU  - Zink, A.
AU  - Hilgers, M.*
AU  - Handrick, C.*
AU  - Quist, S.*
AU  - Schwarz, B.*
AU  - Staubach-Renz, P.*
AU  - Bell, M.*
AU  - Hong-Weldemann, S.H.*
AU  - Homey, B.*
AU  - Brücher, J.J.*
AU  - Schmitt, J.*
AU  - Werfel, T.*
AU  - Weidinger, S.*
C1  - 66034
C2  - 53081
SP  - 1022-1024
TI  - Real-world data on effectiveness, safety and drug survival of dupilumab: An analysis from the TREATgermany registry.
JO  - Br. J. Dermatol.
VL  - 187
IS  - 6
PY  - 2022
SN  - 0007-0963
ER  - 

TY  - JOUR
AB  - BACKGROUND: Growing evidence suggests that atopic dermatitis (AD) is associated with an increased risk of depressive disorders and anxiety. However, existing studies were observational and may uncover correlations but cannot easily disentangle non-causal or reverse-causal associations because these associations could be confounded and may not reflect true causal relationships. OBJECTIVES: We carried out a 2-sample Mendelian randomization (MR) study to examine the potential effect of AD on the risk of depressive disorders and anxiety. METHODS: Genetic instruments from the largest available genome-wide association study (GWAS) for AD (10,788 cases, 30,047 controls) were used to investigate the relation to broad depression (170,756 cases, 329,443 controls), major depressive disorder (MDD) (30,603 cases, 143,916 controls) and anxiety (5,580 cases, 11,730 controls). A set of complementary approaches were carried out to assess horizontal pleiotropy and related potential caveats occurring in MR studies. RESULTS: We observed no causal impact of AD on the risk of depressive disorders and anxiety, with close-to-zero effect estimates. The inverse weighted method revealed no associations of AD on broad depression (OR=1.014, P=0.4307), probable MDD (OR=1.004, P=0.5681), ICD-9/10-based MDD (OR=1.001, P=0.4659) or anxiety (OR=1.097, P=0.1801). CONCLUSIONS: In summary, this MR study does not support a causal effect of AD on depression and anxiety.
AU  - Baurecht, H.*
AU  - Welker, C.*
AU  - Baumeister, S.
AU  - Weidnger, S.*
AU  - Meisinger, C.
AU  - Leitzmann, M.F.*
AU  - Emmert, H.*
C1  - 61731
C2  - 50416
CY  - 111 River St, Hoboken 07030-5774, Nj Usa
SP  - 781–786 
TI  - Relationship between atopic dermatitis, depression and anxiety: A two-sample mendelian randomization study.
JO  - Br. J. Dermatol.
VL  - 185
PB  - Wiley
PY  - 2021
SN  - 0007-0963
ER  - 

TY  - JOUR
AB  - BACKGROUND: The human skin offers diverse ecosystems for microbial symbionts. However, the factors shaping skin-microbiome interactions are still insufficiently characterized. This contrasts to the broader knowledge about the factors influencing the gut microbiota. OBJECTIVES: We aimed to investigate major patterns of associations of host traits, lifestyle, and environmental factors with skin bacteria in two German populations. METHODS: This is a cross-sectional study with 647 participants from two population-based German cohorts, PopGen (n=294) and KORA FF4 (n=353), totaling 1794 skin samples. The V1-V2 regions of the 16S rRNA gene were sequenced. Associations were tested with two bacterial levels, community (beta-diversity) and 16S rRNA gene amplicon sequence variants (ASVs). RESULTS: We validated known associations of the skin microbiota with skin microenvironment, age, body mass index (BMI) and sex. These factors were associated with beta diversity and abundance of ASVs in PopGen, which was largely replicated in KORA FF4. Most intriguingly, dietary macronutrients and total dietary energy were associated with several ASVs. ASVs were also associated with smoking, alcohol consumption, skin pH, skin type, transepidermal water loss, education, and several environmental exposures, including hours spent outdoors. Associated ASVs included members of the genera Propionibacterium, Corynebacterium, and Staphylococcus. DISCUSSION: We expand the current understanding of factors associated with the skin bacterial community. We show the association of diet with the skin bacteria. Finally, we hypothesize that the skin microenvironment and the host physiology would shape the skin bacterial community at greater extent in comparison to a single skin physiological feature, lifestyle and environmental exposition.
AU  - Moitinho-Silva, L.*
AU  - Boraczynski, N.*
AU  - Emmert, H.*
AU  - Baurecht, H.*
AU  - Szymczak, S.*
AU  - Schulz, H.
AU  - Haller, D.*
AU  - Linseisen, J.
AU  - Gieger, C.
AU  - Peters, A.
AU  - Tittmann, L.*
AU  - Lieb, W.*
AU  - Bang, C.*
AU  - Franke, A.*
AU  - Rodriguez, E.*
AU  - Weidinger, S.*
C1  - 61707
C2  - 50406
CY  - 111 River St, Hoboken 07030-5774, Nj Usa
SP  - 573-584
TI  - Host traits, lifestyle and environment are associated with the human skin bacteria.
JO  - Br. J. Dermatol.
VL  - 185
IS  - 3
PB  - Wiley
PY  - 2021
SN  - 0007-0963
ER  - 

TY  - JOUR
AU  - Standl, M.
C1  - 62831
C2  - 51005
CY  - 111 River St, Hoboken 07030-5774, Nj Usa
TI  - Addressing the causality of the association of atopic dermatitis with depression and anxiety using Mendelian randomization.
JO  - Br. J. Dermatol.
PB  - Wiley
PY  - 2021
SN  - 0007-0963
ER  - 

TY  - JOUR
AB  - The German atopic eczema (AE)-registry TREATgermany is a non-interventional multicenter patient cohort study for adult patients with currently moderate-to-severe disease activity or current/previous anti-inflammatory systemic treatment.1,2 Dupilumab has demonstrated to be an effective treatment for patients with moderate-to-severe AE in clinical trials.3-5 Real world evidence is now needed to evaluate its effectiveness and safety in routine care.
AU  - Abraham, S.*
AU  - Haufe, E.*
AU  - Harder, I.*
AU  - Heratizadeh, A.*
AU  - Kleinheinz, A.*
AU  - Wollenberg, A.*
AU  - Weisshaar, E.*
AU  - Augustin, M.*
AU  - Wiemers, F.*
AU  - Zink, A.
AU  - Biedermann, T.
AU  - von Kiedrowski, R.*
AU  - Hilgers, M.*
AU  - Worm, M.*
AU  - Pawlak, M.*
AU  - Sticherling, M.*
AU  - Fell, I.*
AU  - Handrick, C.*
AU  - Schäkel, K.*
AU  - Staubach, P.*
AU  - Asmussen, A.*
AU  - Schwarz, B.*
AU  - Bell, M.*
AU  - Neubert, K.*
AU  - Effendy, I.*
AU  - Bieber, T.*
AU  - Homey, B.*
AU  - Gerlach, B.*
AU  - Tchitcherina, E.*
AU  - Stahl, M.*
AU  - Schwichtenberg, U.*
AU  - Rossbacher, J.*
AU  - Buck, P.*
AU  - Mempel, M.*
AU  - Beissert, S.*
AU  - Werfel, T.*
AU  - Weidinger, S.*
AU  - Schmitt, J.*
AU  - TREATgermany study group*
C1  - 58272
C2  - 48184
TI  - Implementation of dupilumab in routine care of atopic eczema. Results from the German national registry TREATgermany.
JO  - Br. J. Dermatol.
PY  - 2020
SN  - 0007-0963
ER  - 

TY  - JOUR
AB  - Phototesting is used to assess individual sensitivity to ultraviolet (UV) radiation in order to determine adequate UV dosage for phototherapy1 . In the standard procedure, small skin areas are exposed to increasing doses of UV radiation. The lowest UV dose that induces a delineated erythema at 24±2 h after UV exposure defines the minimal erythema dose (MED)2 . Visual assessment is the gold standard for MED determination; however, it is prone to observer variability3 . Optical methods have been considered to quantify the magnitude of erythema response. However, they are limited by light scattering therefore high-resolution is restricted to depths of <200 μm resulting in unreliable measurements4,5 .
AU  - Hindelang, B.
AU  - Aguirre Bueno, J.
AU  - Berezhnoi, A.
AU  - Biedermann, T.*
AU  - Darsow, U.*
AU  - Eberlein, B.*
AU  - Ntziachristos, V.
C1  - 59878
C2  - 49085
CY  - 111 River St, Hoboken 07030-5774, Nj Usa
TI  - Quantification of skin sensitivity to ultraviolet radiation using ultra-wideband optoacoustic mesoscopy.
JO  - Br. J. Dermatol.
PB  - Wiley
PY  - 2020
SN  - 0007-0963
ER  - 

TY  - JOUR
AB  - Patients with atopic dermatitis (AD) have an increased risk of bacterial skin infections, which cause significant morbidity and, if untreated, may become systemic. Staphylococcus aureus colonizes the skin of most patients with AD and is the most common organism to cause infections. Overt bacterial infection is easily recognized by the appearance of weeping lesions, honey-coloured crusts and pustules. However, the wide variability in clinical presentation of bacterial infection in AD and the inherent features of AD – cutaneous erythema and warmth, oozing associated with oedema, and regional lymphadenopathy – overlap with those of infection, making clinical diagnosis challenging. Furthermore, some features may be masked because of anatomical site- and skin-type-specific features, and the high frequency of S. aureus colonization in AD makes positive skin swab culture of suspected infection unreliable as a diagnostic tool. The host mechanisms and microbial virulence factors that underlie S. aureus colonization and infection in AD are incompletely understood. The aim of this article is to present the latest evidence from animal and human studies, including recent microbiome research, to define the clinical features of bacterial infections in AD, and to summarize our current understanding of the host and bacterial factors that influence microbial colonization and virulence.
AU  - Alexander, H.*
AU  - Paller, A.S.*
AU  - Traidl-Hoffmann, C.
AU  - Beck, L.A.*
AU  - de Benedetto, A.*
AU  - Dhar, S.*
AU  - Girolomoni, G.*
AU  - Irvine, A.D.*
AU  - Spuls, P.*
AU  - Su, J.*
AU  - Thyssen, J.P.*
AU  - Vestergaard, C.*
AU  - Werfel, T.*
AU  - Wollenberg, A.*
AU  - Deleuran, M.*
AU  - Flohr, C.*
C1  - 57254
C2  - 47624
CY  - 111 River St, Hoboken 07030-5774, Nj Usa
SP  - 1331-1342
TI  - The role of bacterial skin infections in atopic dermatitis: Expert statement and review from the International Eczema Council Skin Infection Group.
JO  - Br. J. Dermatol.
VL  - 182
IS  - 6
PB  - Wiley
PY  - 2019
SN  - 0007-0963
ER  - 

TY  - JOUR
AB  - BackgroundAs lipids are known to regulate macrophage functions, it is reasonable to suppose that a sebocyte-macrophage axis mediated by sebum lipids may exist. ObjectivesTo investigate if sebocytes could contribute to the differentiation, polarization and function of macrophages with their secreted lipids. MethodsOil Red O lipid staining and Raman spectroscopy were used to assess the dermal lipid content and penetration. Immunohistochemistry was used to analyse the macrophage subsets. Human peripheral blood monocytes were differentiated in the presence of either supernatant from human SZ95 sebocytes or major sebum lipid components and activated with Propionibacterium acnes. Macrophage surface markers and their capacity to uptake fluorescein isothiocyanate-conjugated P.acnes were detected by fluorescence-activated cell sorting measurements. Cytokine protein levels were evaluated by enzyme-linked immunosorbent assay and Western blot analysis. ResultsSebaceous gland-rich skin had an increased dermal lipid content vs. sebaceous gland-poor skin to which all the tested sebum component lipids could contribute by penetrating the dermoepidermal barrier. Of the lipids, oleic acid and linoleic acid promoted monocyte differentiation into alternatively activated macrophages. Moreover, linoleic acid also had an anti-inflammatory effect in P.acnes-activated macrophages, inhibiting the secretion of interleukin (IL)-1, IL-6 and tumour necrosis factor (TNF)-. Squalene, palmitic acid, stearic acid and oleic acid augmented the secretion of IL-1, even in the absence of P. acnes, whereas oleic acid had a selective effect of inducing IL-1 but downregulating IL-6 and TNF- secretion. ConclusionsOur results suggest a role for sebaceous glands in modulating innate immune responses via their secreted lipids that are of possible pathological and therapeutic relevance. What's already known about this topic? The primary function of human sebaceous glands is to produce and secrete sebum, which, so far, has been considered to only contribute to the lipid barrier of the skin. What does this study add? Our work indicates that sebocyte-derived lipids may also target macrophage differentiation and activation. Moreover, in the pathogenesis of acne, Propionibacterium acnes-macrophage interaction might be largely dependent on the composition of the sebum, which is of possible pathological and therapeutic relevance. What is the translational message? These findings open several new avenues for research to consider (sebum) lipids, as well as cytokines, in basic and therapeutic research. The analysis of sebum lipid fractions should be addressed from the scope of their potential immunoregulatory functions under various pathological conditions. Plain language summary available online Respond to this article
AU  - Lovászi, M.*
AU  - Mattii, M.
AU  - Eyerich, K.*
AU  - Gácsi, A.*
AU  - Csányi, E.*
AU  - Kovács, D.*
AU  - Rühl, R.*
AU  - Szegedi, A.*
AU  - Kemény, L.V.*
AU  - Ståhle, M.*
AU  - Zouboulis, C.C.*
AU  - Eyerich, S.
AU  - Törőcsik, D.*
C1  - 51388
C2  - 43203
CY  - Hoboken
SP  - 1671-1682
TI  - Sebum lipids influence macrophage polarization and activation.
JO  - Br. J. Dermatol.
VL  - 177
IS  - 6
PB  - Wiley
PY  - 2017
SN  - 0007-0963
ER  - 

TY  - JOUR
AB  - BackgroundThe main function of sebocytes is considered to be the production of lipids to moisturize the skin. However, it recently became apparent that sebocytes release chemokines and cytokines and respond to proinflammatory stimuli as well as the presence of bacteria. ObjectivesTo analyse the functional communication between human sebocytes and T cells. MethodsImmunofluorescence stainings for CD4 and interleukin (IL)-17 were performed on acne sections and healthy skin. Migration assays and T-cell-stimulation cultures were performed with supernatants derived from unstimulated or prestimulated SZ95 sebocytes. Dendritic cells were generated in the presence of SZ95 supernatant and subsequently used in mixed leucocyte reactions. ResultsWe showed that CD4(+) IL-17(+) T cells accumulate around the pilosebaceous unit and are in close contact with sebocytes in acne lesions. By using SZ95 sebocyte supernatant, we demonstrate a chemotactic effect of sebocytes on neutrophils, monocytes and T cells in a CXCL8-dependent manner. Furthermore, sebocyte supernatant induces the differentiation of CD4(+) CD45RA(+) naive T cells into T helper (Th)17 cells via the secretion of IL-6, transforming growth factor- and, most importantly, IL-1. No direct effects of sebocytes on the function of CD4(+) CD45RO(+) memory T cells were detected. Moreover, sebocytes functionally interact with Propionibacterium acnes in the maturation of dendritic cells, leading to antigen-presenting cells that preferentially prime Th17 cells. ConclusionsOur study provides evidence that human sebocytes actively participate in inflammatory processes in the skin by recruiting and communicating with immune cells. This interaction leads to the generation of Th17 cells, which might contribute to the pathogenesis not only of acne vulgaris, but also of several inflammatory skin diseases. What's already known about this topic? Sebocytes are part of the pilosebaceous unit and produce lipids for moisturizing the skin. They were long regarded as bystander cells during skin inflammation with no impact on the immune response. What does this study add? We show that sebocytes actively contribute to inflammatory processes by recruitment of immune cells into the skin and by skewing T-cell differentiation towards T helper 17 cells. What is the translational message? This interaction of sebocytes might be important in the pathogenesis of other inflammatory diseases. Plain language summary available online Respond to this article
AU  - Mattii, M.
AU  - Lovászi, M.*
AU  - Garzorz, N.V.*
AU  - Atenhan, A.
AU  - Quaranta, M.
AU  - Lauffer, F.*
AU  - Konstantinow, A.*
AU  - Küpper, M.
AU  - Zouboulis, C.C.*
AU  - Kemeny, L.*
AU  - Eyerich, K.*
AU  - Schmidt-Weber, C.B.
AU  - Törőcsik, D.*
AU  - Eyerich, S.
C1  - 51713
C2  - 43462
CY  - Hoboken
SP  - 722-730
TI  - Sebocytes contribute to skin inflammation by promoting the differentiation of T helper 17 cells.
JO  - Br. J. Dermatol.
VL  - 178
IS  - 3
PB  - Wiley
PY  - 2017
SN  - 0007-0963
ER  - 

TY  - JOUR
AU  - Verma, S.B.*
AU  - Mittal, A.*
AU  - Wollina, U.*
AU  - Eckstein, G.N.
AU  - Gohel, K.*
AU  - Giehl, K.
C1  - 50454
C2  - 42459
CY  - Hoboken
SP  - 545-548
TI  - Chanarin-Dorfman syndrome with rare renal involvement.
JO  - Br. J. Dermatol.
VL  - 176
IS  - 2
PB  - Wiley
PY  - 2017
SN  - 0007-0963
ER  - 

TY  - JOUR
AB  - BACKGROUND: Chronic hand eczema is a frequent skin disease with a high socio-economic impact. While some light has been shed on genetic factors predisposing for the disease, little is known on its actual pathogenesis. OBJECTIVES: We therefore set out to systematically and comprehensively analyze the differential protein expression in chronic hand eczema using modern mass spectrometry. METHODS: We performed LC-MS/MS analyses and label-free quantification to analyze the proteomic profile of palmar skin from 12 individuals, 6 hand eczema patients and 6 healthy volunteers. Immunohistochemistry of palmar skin from 7 different hand eczema patients and 7 different healthy volunteers was performed in a second step. RESULTS: With this method we were able to identify 185 candidate proteins whose abundance was significantly different in the hand eczema samples. Among them we found several barrier proteins: filaggrin, filaggrin2 and hornerin all were down-regulated in the hand eczema samples as were the desquamation-related enzymes kallikrein-related peptidase 5 and 7 as well as cystatin E/M. The antimicrobial peptides S100A7 and S100A8/A9 as well as the small proline-rich protein 2B and S100A11 were up-regulated in diseased skin. Immunohistochemistry confirmed these findings. CONCLUSIONS: Our results corroborate the assumption that skin barrier dysfunction plays an essential role in the pathogenesis of chronic hand eczema.
AU  - Molin, S.
AU  - Merl, J.
AU  - Dietrich, K.A.*
AU  - Regauer, M.*
AU  - Flaig, M.*
AU  - Letulé, V.*
AU  - Saucke, T.*
AU  - Herzinger, T.*
AU  - Ruzicka, T.*
AU  - Hauck, S.M.
C1  - 32342
C2  - 35005
CY  - Hoboken
SP  - 994-1001
TI  - The hand eczema proteome: Imbalance of epidermal barrier proteins.
JO  - Br. J. Dermatol.
VL  - 172
IS  - 4
PB  - Wiley-blackwell
PY  - 2015
SN  - 0007-0963
ER  - 

TY  - JOUR
AU  - Garzorz-Stark, N.*
AU  - Knapp, B.
AU  - Quaranta, M.
AU  - Mattii, M.
AU  - Theis, F.J.
AU  - Ring, J.*
AU  - Schmidt-Weber, C.B.
AU  - Eyerich, S.
AU  - Eyerich, K.*
C1  - 31821
C2  - 34791
CY  - Hoboken
SP  - E19
TI  - Eczema ≠ eczema: The transcriptional heterogeneity of eczema.
JO  - Br. J. Dermatol.
VL  - 170
IS  - 6
PB  - Wiley-Blackwell
PY  - 2014
SN  - 0007-0963
ER  - 

TY  - JOUR
AU  - Quaranta, M.*
AU  - Knapp, B.
AU  - Garzoz, N.*
AU  - Mattii, M.
AU  - Pullabhatla, V.*
AU  - Pennino, D.*
AU  - Andres, C.*
AU  - Traidl-Hoffmann, C.*
AU  - Cavani, A.*
AU  - Theis, F.J.*
AU  - Ring, J.*
AU  - Schmidt-Weber, C.B.
AU  - Eyerich, S.
AU  - Eyerich, K.*
C1  - 43243
C2  - 36291
CY  - Hoboken
SP  - E135
TI  - Intraindividual genome expression analysis reveals opposing immune reaction in psoriasis and eczema.
JO  - Br. J. Dermatol.
VL  - 171
IS  - 6
PB  - Wiley-blackwell
PY  - 2014
SN  - 0007-0963
ER  - 

TY  - JOUR
AU  - Cifuentes, L.*
AU  - Kiritsi, D.*
AU  - Chen, W.*
AU  - Pennino, J.
AU  - Ring, J.*
AU  - Weidinger, S.*
AU  - Has, C.*
C1  - 26102
C2  - 32070
SP  - 195-198
TI  - A case of junctional epidermolysis bullosa with prurigo-like lesions and reduction of collagen XVII and filaggrin.
JO  - Br. J. Dermatol.
VL  - 169
IS  - 1
PB  - Wiley-Blackwell
PY  - 2013
SN  - 0007-0963
ER  - 

TY  - JOUR
AB  - Background Ultraviolet (UV) A1 phototherapy is an effective anti-inflammatory treatment modality that influences fibroblast functions. Objectives To document the effects of UVA1 treatment in patients with localized scleroderma (LS) in a retrospective study (at least 6 months after UVA1 treatment) and in a prospective study before and immediately after medium-dose UVA1 irradiation. Methods In total, 30 patients (retrospective study n = 17, prospective study n = 13) with LS receiving UVA1 phototherapy five times weekly (for 3-6 weeks) were investigated. Improvement was documented using standardized questionnaires and clinical evaluation (using modified Rodnan skin score, Cutometer and 7.5-MHz ultrasound measurements). Levels of collagen I and collagen III metabolites were measured in serum and urine. Results In the retrospective study, medium-dose UVA1 phototherapy had been performed 6 months-3 years earlier (cumulative dose 750-1400 J cm(-2); mean +/- SD number of irradiations 19.3 +/- 3.8). Fourteen of 17 patients (82%) reported an improvement in symptoms following UVA1 therapy. In the prospective study, skin elasticity increased in 77% of the patients following medium-dose UVA1 phototherapy (cumulative dose 750-1250 J cm(-2); mean +/- SD number of irradiations 20.8 +/- 4.0). 7.5-MHz ultrasound measurements showed a mean reduction of lesional skin thickness of 13% compared with skin thickness before UVA1 phototherapy. The ratio of deoxypyridinoline to creatinine was significantly elevated in about two-thirds of the patients. Conclusions This open study showed a positive short-and long-term efficacy of UVA1 phototherapy in patients with LS, with a reduction in sclerotic plaques, an increase in skin elasticity and a reduction of lesional skin thickness. UVA1 phototherapy had a significant effect on collagen metabolism. UVA1 phototherapy can be regarded as a safe treatment modality for patients with LS.
AU  - Andres, C.*
AU  - Kollmar, A.*
AU  - Mempel, M.
AU  - Hein, R.*
AU  - Ring, J.*
AU  - Eberlein, B.
C1  - 6144
C2  - 28223
CY  - Malden
SP  - 445-447
TI  - Successful ultraviolet A1 phototherapy in the treatment of localized scleroderma: A retrospective and prospective study.
JO  - Br. J. Dermatol.
VL  - 162
IS  - 2
PB  - Wiley-Blackwell Publishing
PY  - 2010
SN  - 0007-0963
ER  - 

TY  - JOUR
AB  - BACKGROUND: Exposure to fragrances is increasingly encountered in the environment. Some fragrances are known to be important skin and potential airway sensitizers.OBJECTIVES: We investigated whether patients with contact allergy to isoeugenol (ISO) or hydroxyisohexyl-3-carboxaldehyde (HICC) would react to inhalation exposure at the level of the airways and skin.METHODS: Eleven patients sensitized to ISO and 10 patients sensitized to HICC were exposed for 60 min to 1000 microg m(-3) of these compounds in an exposure chamber at rest, and to geraniol 1000 microg m(-3) as a control. Patients wore protective clothing to prevent skin exposure. Assessments were performed prior to exposure, and immediately, 2, 5, 24 and 72 h afterwards. There were no significant changes in lung function but a tendency towards an increased bronchial hyper-responsiveness after exposure to any of the compounds. Laboratory parameters of inflammation did not indicate responses. Single patients reported respiratory symptoms unrelated to objective measures. In contrast, the observed skin symptoms corresponded to the patients' specific sensitization. Four patients reported symptoms compatible with delayed-type hypersensitivity, and two demonstrated a flare after ISO. On re-exposure they did not respond to a lower, more realistic level of ISO.Inhalation of high concentrations of fragrance contact allergens apparently poses a risk for some patients of developing manifest haematogenic contact dermatitis, while the changes in the respiratory tract are limited to symptoms in some subjects without objective changes.
AU  - Schnuch, A.*
AU  - Oppel, E.*
AU  - Oppel, T.*
AU  - Römmelt, H.*
AU  - Kramer, M.*
AU  - Riu, E.*
AU  - Darsow, U.
AU  - Pzybilla, B.*
AU  - Nowak, D.*
AU  - Jörres, R.A.*
C1  - 5828
C2  - 27890
SP  - 598-606
TI  - Experimental inhalation of fragrance allergens in predisposed subjects: Effects on skin and airways.
JO  - Br. J. Dermatol.
VL  - 162
IS  - 3
PB  - Wiley-Blackwell
PY  - 2010
SN  - 0007-0963
ER  - 

TY  - JOUR
AB  - Prognosis of patients with melanoma is strongly associated with tumour thickness at time of diagnosis. Therefore, knowledge of patient characteristics and behaviour associated with a high tumour thickness is essential for the development and improvement of melanoma prevention campaigns. The present study aimed to identify sociodemographic, clinical and behavioural factors associated with high tumour thickness according to Breslow. The study population consisted of 217 patients with histologically proven primary invasive cutaneous melanomas seen at the Department of Dermatology and Allergology at the Ludwig-Maximilian-University Munich, Germany, between January 1999 and January 2001. Personal interviews were conducted by two physicians to obtain information on sociodemographic characteristics and on patients’ knowledge of melanoma symptoms, sun behaviour, delay in diagnosis and related factors. Multivariate linear and logistic regression analysis with stepwise variable selection was used to identify risk groups with a high tumour thickness. To assess possible effect modifications, interaction terms were included in the regression analysis.Results The median tumour thickness was 0·8 mm (interquartile range 0·5–1·6). Fifty-seven patients (26%) had tumour thickness >1·5 mm. In a multivariate linear regression analysis, patients living alone and patients with a low educational level showed a significantly greater tumour thickness. The relation of melanoma knowledge to tumour thickness was modified by the melanoma subtype: whereas lack of melanoma knowledge led to an increased tumour thickness for the subtypes superficial spreading melanoma, lentigo maligna melanoma and unspecified malignant melanoma, no significant effect was estimated for the subtypes nodular melanoma (NM) and acrolentiginous melanoma (ALM). Sex, age, self-detection of melanoma, patient delay and professional delay were not significantly associated with the tumour thickness in multivariate linear regression. Similar results were found in multivariate logistic regression. An increased tumour thickness was found in subjects living alone and having a low educational level. These subjects should be targeted in future prevention campaigns in a more focused way. Further efforts are necessary to improve knowledge and earlier detection of melanoma subtypes NM and ALM.
AU  - Baumert, J.J.
AU  - Plewig, G.*
AU  - Volkenandt, M.*
AU  - Schmid-Wendtner, M.-H.*
C1  - 1273
C2  - 24621
SP  - 938-944
TI  - Factors associated with a high tumour thickness in patients with melanoma.
JO  - Br. J. Dermatol.
VL  - 156
IS  - 5
PB  - Wiley-Blackwell
PY  - 2007
SN  - 0007-0963
ER  - 

TY  - JOUR
AU  - Krämer, U.
AU  - Lemmen, C.
AU  - Bartusel, E.*
AU  - Link, E.*
AU  - Ring, J.*
AU  - Behrendt, H.
C1  - 5536
C2  - 23155
SP  - 99-105
TI  - Current eczema in children is related to Der f 1 exposure but not to Der p 1 exposure.
JO  - Br. J. Dermatol.
VL  - 154
PB  - Wiley
PY  - 2006
SN  - 0007-0963
ER  - 

TY  - JOUR
AU  - Möhrenschlager, M.*
AU  - Schäfer, T.*
AU  - Huss-Marp, J.
AU  - Eberlein-König, B.*
AU  - Weidinger, S.*
AU  - Ring, J.*
AU  - Behrendt, H.
AU  - Krämer, U.
C1  - 5680
C2  - 23636
SP  - 505-513
TI  - The course of eczema in children aged 5-7 years and its relation to atopy: Differences between boys and girls.
JO  - Br. J. Dermatol.
VL  - 154
PB  - Wiley
PY  - 2006
SN  - 0007-0963
ER  - 

TY  - JOUR
AU  - Braun-Falco, M.
AU  - Fischer, S.*
AU  - Plötz, S.G.
AU  - Ring, J.
C1  - 2858
C2  - 22944
SP  - 1103-1104
TI  - Angiolymphoid hyperplasia with eosinophilia treated with anti-interleukin-5 antibody (mepolizumab).
JO  - Br. J. Dermatol.
VL  - 151
PB  - Wiley
PY  - 2004
SN  - 0007-0963
ER  - 

TY  - JOUR
AB  - Background The negative impact of environmental tobacco smoke (ETS) on airway diseases in children is well known. Whether there is an effect on atopic eczema is not clear. Objectives To determine the impact of ETS on atopic eczema, allergic sensitization and allergic airway diseases in 1669 school beginners. Methods The prevalence of atopy-related health outcomes was assessed by questionnaire, dermatological examination, skin prick testing and specific immunoglobulin E measurement. Exposure assessments were based on measurement of cotinine [expressed as cotinine to creatine ratio (CCR)] in spot urine samples (n = 1220) together with questionnaire and interview data on smoking behaviour of the parents. Results In the total study group, prevalence of atopic eczema diagnosed on examination was significantly associated with urinary CCR values. The odds ratio (OR) and 95% confidence interval (CI), calculated for an increase of 100 ng mg(-1) CCR was 1.97 (95% CI 1.23-3.16). The prevalence of skin manifestations according to questionnaire data as well as a history of asthma, wheezing, and hay fever were positively although not significantly associated with ETS exposure. When genetically predisposed children (defined by the presence of parental atopy) were compared with children whose parents had no atopy, the ORs of allergic outcome variables were generally higher in the first group. In the group of predisposed children, significant associations with urinary CCR were found for allergic sensitization against house dust mites as measured by skin prick test (OR 3.10, 95% CI 1.63-5.90). Conclusions Children are at a higher risk of developing an atopic eczema when exposed to ETS and genetically predisposed children are at higher risk of developing a sensitization against house dust mites.
AU  - Krämer, U.*
AU  - Lemmen, C.
AU  - Behrendt, H.
AU  - Link, E.
AU  - Schäfer, T.*
AU  - Gostomzyk, J.*
AU  - Scherer, G.*
AU  - Ring, J.*
C1  - 3065
C2  - 21743
SP  - 111-118
TI  - The effect of environmental tobacco smoke on eczema and allergic sensitization in children.
JO  - Br. J. Dermatol.
VL  - 150
IS  - 1
PB  - Wiley
PY  - 2004
SN  - 0007-0963
ER  -