TY  - JOUR
AB  - In skeletal muscle, the Hippo effector Yap promotes satellite cell, myoblast, and rhabdomyoblast proliferation but prevents myogenic differentiation into multinucleated muscle fibres. We previously noted that Yap drives expression of the first enzyme of the serine biosynthesis pathway, phosphoglycerate dehydrogenase (Phgdh). Here, we examined the regulation and function of Phgdh in satellite cells and myoblasts and found that Phgdh protein increased during satellite cell activation. Analysis of published data reveal that Phgdh mRNA in mouse tibialis anterior muscle was highly expressed at day 3 of regeneration after cardiotoxin injection, when markers of proliferation are also robustly expressed and in the first week of synergist-ablated muscle. Finally, siRNA-mediated knockdown of PHGDH significantly reduced myoblast numbers and the proliferation rate. Collectively, our data suggest that Phgdh is a proliferation-enhancing metabolic enzyme that is induced when quiescent satellite cells become activated.
AU  - Meinhold, M.*
AU  - Verbrugge, S.*
AU  - Shi, A.*
AU  - Schönfelder, M.*
AU  - Becker, L.
AU  - Jaspers, R.T.*
AU  - Zammit, P.S.*
AU  - Wackerhage, H.*
C1  - 68989
C2  - 55172
CY  - One New York Plaza, Suite 4600, New York, Ny, United States
SP  - 271–283
TI  - Yap/Taz activity is associated with increased expression of phosphoglycerate dehydrogenase that supports myoblast proliferation.
JO  - Cell Tissue Res.
VL  - 395
PB  - Springer
PY  - 2024
SN  - 0044-3794
ER  - 

TY  - JOUR
AB  - Stroke is the leading cause of adult disability. Endogenous neural stem/progenitor cells (NSPCs) originating from the subventricular zone (SVZ) contribute to the brain repair process. However, molecular mechanisms underlying CNS disease-induced SVZ NSPC-redirected migration to the lesion area are poorly understood. Here, we show that genetic depletion of the p75 neurotrophin receptor (p75NTR−/−) in mice reduced SVZ NSPC migration towards the lesion area after cortical injury and that p75NTR−/− NSPCs failed to migrate upon BDNF stimulation in vitro. Cortical injury rapidly increased p75NTR abundance in SVZ NSPCs via bone morphogenetic protein (BMP) receptor signaling. SVZ-derived p75NTR−/− NSPCs revealed an altered cytoskeletal network- and small GTPase family-related gene and protein expression. In accordance, BMP-treated non-migrating p75NTR−/− NSPCs revealed an altered morphology and α-tubulin expression compared to BMP-treated migrating wild-type NSPCs. We propose that BMP-induced p75NTR abundance in NSPCs is a regulator of SVZ NSPC migration to the lesion area via regulation of the cytoskeleton following cortical injury.
AU  - Deshpande, S.S.*
AU  - Malik, S.C.*
AU  - Conforti, P.*
AU  - Lin, J.d.*
AU  - Chu, Y.H.*
AU  - Nath, S.*
AU  - Greulich, F.
AU  - Dumbach, M.A.*
AU  - Uhlenhaut, N.H.
AU  - Schachtrup, C.*
C1  - 63383
C2  - 51360
CY  - One New York Plaza, Suite 4600, New York, Ny, United States
TI  - P75 neurotrophin receptor controls subventricular zone neural stem cell migration after stroke.
JO  - Cell Tissue Res.
PB  - Springer
PY  - 2021
SN  - 0044-3794
ER  - 

TY  - JOUR
AB  - The worldwide prevalence of diabetes mellitus and obesity is rapidly increasing not only in adults but also in children and adolescents. Diabetes is associated with macrovascular complications increasing the risk for cardiovascular disease and stroke, as well as microvascular complications leading to diabetic nephropathy, retinopathy and neuropathy. Animal models are essential for studying disease mechanisms and for developing and testing diagnostic procedures and therapeutic strategies. Rodent models are most widely used but have limitations in translational research. Porcine models have the potential to bridge the gap between basic studies and clinical trials in human patients. This article provides an overview of concepts for the development of porcine models for diabetes and obesity research, with a focus on genetically engineered models. Diabetes-associated ocular, cardiovascular and renal alterations observed in diabetic pig models are summarized and their similarities with complications in diabetic patients are discussed. Systematic multi-organ biobanking of porcine models of diabetes and obesity and molecular profiling of representative tissue samples on different levels, e.g., on the transcriptome, proteome, or metabolome level, is proposed as a strategy for discovering tissue-specific pathomechanisms and their molecular key drivers using systems biology tools. This is exemplified by a recent study providing multi-omics insights into functional changes of the liver in a transgenic pig model for insulin-deficient diabetes mellitus. Collectively, these approaches will provide a better understanding of organ crosstalk in diabetes mellitus and eventually reveal new molecular targets for the prevention, early diagnosis and treatment of diabetes mellitus and its associated complications.
AU  - Renner, S.*
AU  - Blutke, A.
AU  - Clauss, S.*
AU  - Deeg, C.A.*
AU  - Kemter, E.*
AU  - Merkus, D.*
AU  - Wanke, R.*
AU  - Wolf, E.*
C1  - 57831
C2  - 48124
CY  - One New York Plaza, Suite 4600, New York, Ny, United States
SP  - 341-378
TI  - Porcine models for studying complications and organ crosstalk in diabetes mellitus.
JO  - Cell Tissue Res.
VL  - 380
IS  - 2
PB  - Springer
PY  - 2020
SN  - 0044-3794
ER  - 

TY  - JOUR
AU  - Falk, S.
AU  - Karow, M.*
C1  - 52014
C2  - 43664
CY  - New York
SP  - 181–187
TI  - Natural and forced neurogenesis: Similar and yet different?
JO  - Cell Tissue Res.
VL  - 371
IS  - 1
PB  - Springer
PY  - 2017
SN  - 0044-3794
ER  - 

TY  - JOUR
AB  - Neuronal differentiation relies on a set of interconnected molecular events to achieve the differentiation of pan-neuronal hallmarks, together with neuronal subtype-specific features. Here, we propose a conceptual framework for these events, based on recent findings. This framework encompasses a dimension in time during development, progressing from early master regulators to later expressed effector genes and terminal selector genes. As a horizontal intersection, we propose the action of permissive fate determinants that are critical in allowing progression through the above transcriptional phases. Typically, these are widely expressed and often interact with the chromatin remodeling machinery. We conclude by discussing this model in the context of the direct fate conversion of various somatic cells into neurons.
AU  - Ninkovic, J.
AU  - Götz, M.
C1  - 42829
C2  - 35423
CY  - New York
SP  - 5-16
TI  - How to make neurons-thoughts on the molecular logic of neurogenesis in the central nervous system.
JO  - Cell Tissue Res.
VL  - 359
IS  - 1
PB  - Springer
PY  - 2015
SN  - 0044-3794
ER  - 

TY  - JOUR
AB  - Since their discovery in the early 1990s, microRNAs have emerged as key components of the post-transcriptional regulation of gene expression. MicroRNAs occur in the plant and animal kingdoms, with the numbers of microRNAs encoded in the genome increasing together with the evolutionary expansion of the phyla. By base-pairing with complementary sequences usually located within the 3' untranslated region, microRNAs target mRNAs for degradation, destabilization and/or translational inhibition. Because one microRNA can have many, if not hundreds, of target mRNAs and because one mRNA can, in turn, be targeted by many microRNAs, these small single-stranded RNAs can exert extensive pleiotropic functions during the development, adulthood and ageing of an organism. Specific functions of an increasing number of microRNAs have been described for the invertebrate and vertebrate nervous systems. Among these, the miR-8/miR-200 microRNA family has recently emerged as an important regulator of neurogenesis and gliogenesis and of adult neural homeostasis in the central nervous system of fruit flies, zebrafish and rodents. This highly conserved microRNA family consists of a single ortholog in the fruit fly (miR-8) and five members in vertebrates (miR-200a, miR-200b, miR-200c, miR-141 and miR-429). Here, we review our current knowledge about the functions of the miR-8/miR-200 microRNA family during invertebrate and vertebrate neural development and adult homeostasis and, in particular, about their role in the regulation of neural stem/progenitor cell proliferation, cell cycle exit, transition to a neural precursor/neuroblast state, neuronal differentiation and cell survival and during glial cell growth and differentiation into mature oligodendrocytes.
AU  - Trümbach, D.
AU  - Prakash, N.
C1  - 31515
C2  - 34522
CY  - New York
SP  - 161-177
TI  - The conserved miR-8/miR-200 microRNA family and their role in invertebrate and vertebrate neurogenesis.
JO  - Cell Tissue Res.
VL  - 359
IS  - 1
PB  - Springer
PY  - 2015
SN  - 0044-3794
ER  - 

TY  - JOUR
AB  - The bursa of Fabricius of the chicken is known as a primary lymphoid organ for B-cell development. Morphologically, the origin of IgG-containing cells in the bursa has not been clear until now, because abundant maternal IgG (MIgG) is transported to the chick embryo and distributed to the bursal tissue around hatching. Thus, it has been difficult to find out whether these cells themselves biosynthesize IgG or if they acquire MIgG via attachment to their surface. Our present study employing in situ hybridization clarified that IgG-containing cells in the medulla of bursal follicles did not biosynthesize IgG. To study the role of MIgG in the development of those IgG-containing cells, MIgG-free chicks were established from surgically bursectomized hen (SBx-hen). We found that, on the one hand, deprivation of MIgG from chicks completely inhibited the development of IgG-containing cells in the medulla after hatching. On the other hand, administration of MIgG to MIgG-free chicks recovered the emergence of those cells. In addition, we observed that those cells did not bear a B-cell marker and possessed dendrites with aggregated IgG. These results demonstrate that IgG-containing cells in the medulla are reticular cells that capture aggregated MIgG. Moreover, we show that the isolation of the bursa from environmental stimuli by bursal duct ligation (BDL) suppressed the development of IgG-containing cells after hatching. Thus, it is implied that environmental stimulations play a key role in MIgG aggregations and dendritic distributions of aggregated MIgG in the medulla after hatching.
AU  - Ekino, S.*
AU  - Arakawa, H.
AU  - Sonoda, K.*
AU  - Noguchi, K.*
AU  - Inui, S.*
AU  - Yokoyama, H.*
AU  - Kodama, Y.*
C1  - 7572
C2  - 29896
SP  - 537-550
TI  - The origin of IgG-containing cells in the bursa of Fabricius.
JO  - Cell Tissue Res.
VL  - 348
IS  - 3
PB  - Springer
PY  - 2012
SN  - 0044-3794
ER  - 

TY  - JOUR
AB  - Wnt proteins have now been identified as major physiological regulators of multiple aspects of stem cell biology, from self-renewal and pluripotency to precursor cell competence and terminal differentiation. Neural stem cells are the cellular building blocks of the developing nervous system and provide the basis for continued neurogenesis in the adult mammalian central nervous system. Here, we outline the most recent advances in the field about the critical factors and regulatory networks involved in Wnt signaling and discuss recent findings on how this increasingly intricate pathway contributes to the shaping of the developing and adult nervous system on the level of the neural stem cell.
AU  - Michaelidis, T.M.
AU  - Lie, D.C.
C1  - 730
C2  - 25123
SP  - 193-210
TI  - Wnt signaling and neural stem cells: Caught in the Wnt web.
JO  - Cell Tissue Res.
VL  - 331
IS  - 1
PB  - Springer
PY  - 2008
SN  - 0044-3794
ER  - 

TY  - JOUR
AU  - Wah, J.*
AU  - Wellek, A.*
AU  - Frankenberger, M.
AU  - Unterberger, P.*
AU  - Welsch, U.*
AU  - Bals, R.*
C1  - 4470
C2  - 23534
SP  - 449-456
TI  - Antimicrobial peptides are present in immune and host defense cells of the human respiratory and gastrointestinal tracts.
JO  - Cell Tissue Res.
VL  - 324
PY  - 2006
SN  - 0044-3794
ER  - 

TY  - JOUR
AU  - Prakash, N.
AU  - Wurst, W.
C1  - 5203
C2  - 22413
SP  - 5-14
TI  - Specification of midbrain territory.
JO  - Cell Tissue Res.
VL  - 318
PY  - 2004
SN  - 0044-3794
ER  - 

TY  - JOUR
AU  - Ziegler, I.
AU  - Epperlein, H.H.
AU  - Perris, R.
C1  - 17299
C2  - 10337
TI  - Identification of Pigment Cells During Early Amphibien Development.
JO  - Cell Tissue Res.
PY  - 1988
SN  - 0044-3794
ER  - 

TY  - JOUR
AB  - Nocodazole, a temporary inhibitor of microtubule formation, has been used to partly synchronize Ehrlich ascites tumour cells growing in suspension. the gradual entry of cells into mitosis and into the next cell cycle without division during drug treatment has been studied by flow cytometric determination of mitotic cells, analysing red and green fluorescence after low pH treatment and acridine orange staining. Determination of the mitotic index (MI) by this method has been combined with DNA distribution analysis to measure cell-cycle phase durations in asynchronous populations growing in the presence of the drug. With synchronized cells, it was shown that in the concentration range 0.4–4.0 μg/l, cells could only be arrested in mitosis for about 7 hr and at 0.04 μg/ml, for about 5 hr. After these time intervals, the DNA content in nocodazole-blocked cells was found to be increased, and, in parallel, the ratio of red and green fluorescence was found to have changed, showing entry of cells into a next cell cycle without division (polyploidization). It was therefore only possible to partially synchronize an asynchronous population by nocodazole. However, a presynchronized population, e.g. selected G1 cells or metabolically blocked G1/S cells, were readily and without harmful effect resynchronized in M phase by a short treatment (0.4 μg/ml, 3–4 hr) with nocodazole; after removal of the drug, cells divided and progressed in a highly synchronized fashion through the next cell cycle.
AU  - Nüsse, M.
AU  - Egner, H.J.
C1  - 41432
C2  - 40427
SP  - 13-23
TI  - Can nocodazole, an inhibitor of microtubule formation, be used to synchronize mammalian cells? Accumulation of cells in mitosis studied by two parametric flow cytometry using acridine orange and by DNA distribution analysis.
JO  - Cell Tissue Res.
VL  - 17
IS  - 1
PY  - 1984
SN  - 0044-3794
ER  - 

TY  - JOUR
AB  - T-lymphocytes and B-lymphocytes are identified in tissue sections of human tonsils by applying the unlabelled antibody enzyme method. The epithelium of the tonsils contains a majority of immunoglobulin-positive cells and fewer T-lymphocytes. In the subepithelial zones, areas composed of B-cells predominate, however, regions containing T-lymphocytes are also present. The latter are mainly arranged in the lamina propria around high-endothelial venules and often include plasma cells containing immunoglobulin. Follicles containing germinal centres display a complex structure which changes during development. The lymphocytic cap consists of densely packed lymphocytes, labelled heavily by anti-IgM and anti-IGD, and of individual T-lymphocytes. Germinal centres show a framework of immunoglobulin-positive dendritic reticular cells; they contain some heavily labelled lymphoid cells and several cells weakly labelled by anti-IgM and anti-IgA, as well as a small number of T-lymphocytes. Furthermore, the total areas of T- and B-lymphocytes measured by planimetry may differ considerably between different tonsils. Especially total areas of germinal centres vary to a great extent. The quantitative data on amounts of T- and B-cells achieved by planimetry are comparable to those reported in cellular suspensions of tonsils.
AU  - Hoffmann-Fezer, G.
AU  - Loehrs, U.
AU  - Rodt, H.V.
AU  - Thierfelder, S.S.
C1  - 41757
C2  - 38574
SP  - 361-375
TI  - Immunohistochemical identification of T-and B-lymphocytes delineated by the unlabelled antibody enzyme method. III. Topographical and quantitative distribution of T-and B-cells in human palatine tonsils.
JO  - Cell Tissue Res.
VL  - 216
IS  - 2
PY  - 1981
SN  - 0044-3794
ER  -