TY  - JOUR
AB  - We evaluated the mechanistic link between circadian rhythms and gut barrier permeability. Mice were subjected to either constant 24-h light (LL) or 12-h light/dark cycles (LD). Mice housed in LL experienced a significant increase in gut barrier permeability that was associated with dysregulated ß-catenin expression and altered expression of tight junction (TJ) proteins. Silencing of ß-catenin resulted in disruption of barrier function in SW480 cells, with ß-catenin appearing to be an upstream regulator of the core circadian components, such as Bmal1, Clock, and Per1/2. In addition, ß-catenin silencing downregulated ZO-1 and occludin TJ proteins with only limited or no changes at their mRNA levels, suggesting post transcriptional regulation. Indeed, silencing of ß-catenin significantly upregulated expression of matrix metallopeptidase (MMP)-2 and MMP-9, and blocking MMP-2/9 activity attenuated epithelial disruption induced by ß-catenin silencing. These results indicate the regulatory role of circadian disruption on gut barrier integrity and the associations between TJ proteins and circadian rhythms, while demonstrating the regulatory role of ß-catenin in this process.
AU  - Eum, S.Y.*
AU  - Schurhoff, N.*
AU  - Teglas, T.*
AU  - Wolff, G.
AU  - Toborek, M.*
C1  - 65951
C2  - 52999
SP  - 581-595
TI  - Circadian disruption alters gut barrier integrity via a ß-catenin-MMP-related pathway.
JO  - Mol. Cell. Biochem.
VL  - 478
IS  - 3
PY  - 2023
SN  - 0300-8177
ER  - 

TY  - JOUR
AU  - Haus-Seuffert, P.
AU  - Meisterernst, M.
C1  - 21615
C2  - 19746
SP  - 5-9
TI  - Mechanisms of transcriptional activation of cAMP-responsive element-binding protein CREB.
JO  - Mol. Cell. Biochem.
VL  - 212
PY  - 2000
SN  - 0300-8177
ER  -