TY  - JOUR
AB  - The 3rd annual meeting on 'Salamander Models in Cross-disciplinary Biological Research' took place online on August 2021, bringing together over 200 international researchers using salamanders as research models and encompassing diverse fields, ranging from Development and Regeneration through to Immunology, Pathogenesis and Evolution. The event was organized by Maximina H. Yun (Center for Regenerative Therapies Dresden, Germany) and Tatiana Sandoval-Guzmán (TU Dresden, Germany) with the generous support of the Deutsche Forschungsgemeinschaft, the Center for Regenerative Therapies Dresden, Technische Universität Dresden, and the Company of Biologists. Showcasing a number of emerging salamander models, innovative techniques and resources, and providing a platform for sharing both published and ongoing research, this meeting proved to be an excellent forum for exchanging ideas and moving research forwards. Here, we discuss the highlights stemming from this exciting scientific event.
AU  - Masselink, W.*
AU  - Sandoval-Guzmán, T.*
AU  - Yun, M.H.*
C1  - 64882
C2  - 52579
SP  - 906-910
TI  - Meeting report: Salamander models in cross-disciplinary biological research meeting.
JO  - Dev. Dyn.
VL  - 251
IS  - 6
PY  - 2022
SN  - 1058-8388
ER  - 

TY  - JOUR
AB  - Background: The contribution of the endoderm to the oral tissues of the head has been debated for many years. With the arrival of Cre/LoxP technology endoderm progenitor cells can now be genetically labeled and tissues derived from the endoderm traced. Using Sox17-2A-iCre/Rosa26 reporter mice we have followed the fate of the endoderm in the teeth, glands, and taste papillae of the oral cavity. Results: No contribution of the endoderm was observed at any stage of tooth development, or in development of the major salivary glands, in the reporter mouse during development. In contrast, the minor mucous glands of the tongue were found to be of endodermal origin, along with the circumvallate papilla and foliate papillae. The mucous minor salivary glands of the palate, however, were of mixed ectodermal and endodermal origin. Conclusions: In contrast to urodele studies, the epithelium of murine teeth is derived solely from the ectoderm. The border between the ectoderm- and endoderm-derived epithelium may play a role in determining the position of the lingual glands and taste buds, and may explain differences observed between taste buds in the anterior and posterior part of the tongue.
AU  - Rothova, M.*
AU  - Thompson, H.*
AU  - Lickert, H.
AU  - Tucker, A.S.*
C1  - 7594
C2  - 29907
SP  - 1183-1191
TI  - Lineage tracing of the endoderm during oral development.
JO  - Dev. Dyn.
VL  - 241
IS  - 7
PB  - Wiley-Blackwell
PY  - 2012
SN  - 1058-8388
ER  - 

TY  - JOUR
AB  - Wnts are known to bind and activate multiple membrane receptors/coreceptors and to regulate dopaminergic (DA) neuron development and ventral midbrain (VM) morphogenesis. The low density lipoprotein receptor-related protein (Lrp6) is a Wnt co-receptor, yet it remains unclear whether Lrp6 is required for DA neuron development or VM morphogenesis. Lrp6 is expressed ubiquitously in the developing VM. In this study, we show that Lrp6(-/-) mice exhibit normal patterning, proliferation and cell death in the VM, but display a delay in the onset of DA precursor differentiation. A transient 50% reduction in tyrosine hydroxylase-positive DA neurons and in the expression of DA markers such as Nurr1 and Pitx3, as well as a defect in midbrain morphogenesis was detected in the mutant embryos at embryonic day 11.5. Our results, therefore, suggest a role for Lrp6 in the onset of DA neuron development in the VM as well as a role in midbrain morphogenesis.
AU  - Castelo-Branco, G.*
AU  - Andersson, E.R.*
AU  - Minina, E.
AU  - Sousa, K.M.*
AU  - Ribeiro, D.*
AU  - Kokubu, C.*
AU  - Imai, K.
AU  - Prakash, N.
AU  - Wurst, W.
AU  - Arenas, E.*
C1  - 6072
C2  - 27873
SP  - 211-221
TI  - Delayed dopaminergic neuron differentiation in Lrp6 mutant mice.
JO  - Dev. Dyn.
VL  - 239
IS  - 1
PB  - Wiley-Blackwell
PY  - 2010
SN  - 1058-8388
ER  - 

TY  - JOUR
AB  - Wnt/beta-catenin signaling controls the proper development of the mid-/hindbrain region (MHR) and of midbrain dopaminergic (mDA) neurons, but the Frizzled (Fzd) receptors transducing these signals are still unknown. Fzd3 is expressed throughout the mouse anterior neural tube, whereas Fzd6 is restricted to the MHR. We show that the MHR is properly established and mDA neurons develop normally in Fzd6(-/-) mutants, but the number of mDA neurons is initially reduced and recovers at later stages in Fzd3(-/-) embryos. Fzd3(-/-); Fzd6(-/-) double mutants exhibit a severe midbrain morphogenesis defect consisting of collapsed brain ventricles, apparent thickening of the neuroepithelium, focal disruption of the ventricular basal lamina and protrusion of individual cells, and increased proliferation at later stages, despite a normal closure of the anterior neural tube and the rescue of the mDA defect in these embryos. Fzd3 and Fzd6 thus control proper midbrain morphogenesis by a yet unknown mechanism in the mouse.
AU  - Stübner, S.
AU  - Faus-Kessler, T.
AU  - Fischer, T.
AU  - Wurst, W.
AU  - Prakash, N.
C1  - 2371
C2  - 27373
SP  - 246-260
TI  - Fzd3 and Fzd6 deficiency results in a severe midbrain morphogenesis defect.
JO  - Dev. Dyn.
VL  - 239
IS  - 1
PB  - Wiley-Blackwell
PY  - 2010
SN  - 1058-8388
ER  - 

TY  - JOUR
AB  - Signaling pathways mediated by receptor tyrosine kinases (RTKs) are under positive and negative regulation, and misregulation of RTK signaling results in developmental defects and malignancy. A major class of antagonists of Fgf and Egf signaling are the Sprouty proteins. Through an enhancer detection approach, we isolated the sprouty1 (spry1) gene, expressed in multiple developing organs during embryogenesis. We analyzed expression of spry1 between tail bud stage and 10 days postfertilization. From the tail bud stage on, transcript and reporter are detected in the craniofacial region and in the mid-hindbrain boundary, where expression persists until adulthood. Further expression domains are the telencephalon, hindbrain, dorsal diencephalon and epiphysis, branchial arches, pituitary, and the tubular gill epithelium. In the trunk spry1 is also prominently expressed in pronephros, the lateral line and tail fin. Sprouty1 acts in Fgf signaling downstream of Fgfr1, as its expression is abrogated through the small molecule inhibitor of this receptor, SU5402.
AU  - Komisarczuk, A.Z.*
AU  - Topp, S.
AU  - Stigloher, C.
AU  - Kapsimali, M.*
AU  - Bally-Cuif, L.
AU  - Becker, T.S.*
C1  - 5079
C2  - 25905
SP  - 2594-2603
TI  - Enhancer detection and developmental expression of zebrafish sprouty1, a member of the fgf8 synexpression group.
JO  - Dev. Dyn.
VL  - 237(9)
IS  - 9
PB  - Wiley-Blackwell
PY  - 2008
SN  - 1058-8388
ER  - 

TY  - JOUR
AB  - Modulation of cell-cell adhesion is crucial for regulating neuronal migration and maintenance of structural plasticity in the embryonic and mature brain. Such modulation can be obtained by the enzymatic attachment of polysialic acid (PSA) to the neural cell adhesion molecule (NCAM) by means of the polysialyltransferases STX and PST. Thus, differential expression of STX and PST is likely to be responsible for varying functions of PSA-NCAM during neuronal differentiation, maintenance, plasticity, and regeneration. We have isolated the zebrafish homologues of STX (St8sia2) and PST (St8sia4) and demonstrate that their expression in the embryonic and adult nervous system is often confined to regions of neuronal migration. Moreover, in the adult cerebellum, the complementary expression pattern of both polysialyltransferases suggests a function in regulating cerebellar neuronal plasticity. Enzymatic removal of PSA in the embryonic cerebellum results in impaired neuronal migration, suggesting that PSA-NCAM is a key regulator of motility for cerebellar neuronal progenitors.
AU  - Rieger, S.
AU  - Volkmann, K.
AU  - Köster, R.W.
C1  - 689
C2  - 25111
SP  - 276-285
TI  - Polysialyltransferase expression is linked to neuronal migration in the developing and adult zebrafish.
JO  - Dev. Dyn.
VL  - 237
IS  - 1
PB  - Wiley-Blackwell
PY  - 2008
SN  - 1058-8388
ER  - 

TY  - JOUR
AB  - To gain knowledge about the developmental origin of serotonergic precursors and the regulatory cascades of serotonergic differentiation in vertebrates, we determined the spatiotemporal expression profile of the Ets-domain transcription factor-encoding gene pet1 in developing and adult zebrafish. We show that it is an early, specific marker of raphe serotonergic neurons, but not of other serotonergic populations. We then use pet1 expression together with tracing techniques to demonstrate that serotonergic neurons of rhombomeres (r) 1-2 largely originate from a progenitor pool at the midbrain-hindbrain boundary. Furthermore, by combining expression analyses of pet1 and the raphe tryptophan hydroxylase (Tph2) with rhombomere identity markers, we show that anterior and posterior hindbrain clusters of serotonergic precursors are separated by r3, rather than r4 as in other vertebrates. Our findings establish the origin of r1-2 serotonergic precursors, and strengthen the evidence for molecular, ontogenic and phylogenic heterogeneities among the vertebrate brain serotonergic cell populations.
AU  - Lillesaar, C.
AU  - Tannhäuser, B.
AU  - Stigloher, C.
AU  - Kremmer, E.
AU  - Bally-Cuif, L.
C1  - 566
C2  - 24748
SP  - 1072-1084
TI  - The serotonergic phenotype is acquired by converging genetic mechanisms within the zebrafish central nervous system.
JO  - Dev. Dyn.
VL  - 236
IS  - 4
PB  - Wiley-Blackwell
PY  - 2007
SN  - 1058-8388
ER  - 

TY  - JOUR
AB  - The Notch signaling pathway is involved in diverse biological processes such as cell fate decisions or stem cell maintenance. In this study, we assessed the role of this pathway for melanocyte development and hair pigmentation using RBP-J kappa, Notch1, and Notch2 conditional knockout mice. Disruption of the Notch pathway by inactivating RBP-J kappa in the melanocyte lineage using Tyr::Cre mice led to a severe coat color dilution. Similarly, hair graying was observed when Notch1 and/or Notch2 receptors were ablated in melanocytes. This phenotype was proportional to the number of floxed Notch alleles, with the most pronounced effect seen in Tyr::Cre/degrees; Notch1(flox/flox); Notch2(flox/flox) mice. Deletion of Notch1 and/or Notch2 in melanoblasts did not induce a congenital defect. The number of Dct-expressing cells at embryonic stages was not affected, but melanocytes located within the hair matrix progressively disappeared during the first regeneration of the hair follicle. In contrast, non-follicular melanocytes and pigmentation in the dermis and in the choroid were not affected. We suggest that both Notch1 and Notch2 receptors contribute to the maintenance of melanoblasts and melanocyte stem cells, and are essential for proper hair pigmentation.
AU  - Schouwey, K.*
AU  - Delmas, V.*
AU  - Larue,L.*
AU  - Zimber-Strobl, U.
AU  - Strobl, L.J.
AU  - Radtke, F.*
AU  - Beermann, F.*
C1  - 4942
C2  - 24181
SP  - 282-289
TI  - Notch1 and Notch2 receptors influence progressive hair graying in a dose-dependent manner.
JO  - Dev. Dyn.
VL  - 236
IS  - 1
PB  - Wiley-Blackwell
PY  - 2007
SN  - 1058-8388
ER  - 

TY  - JOUR
AU  - Distel, M.
AU  - Babaryka, A.
AU  - Köster, R.W.
C1  - 2310
C2  - 23501
SP  - 1100-1106
TI  - Multicolor in vivo time-lapse imaging at cellular resolution by stereomicroscopy.
JO  - Dev. Dyn.
VL  - 235
PB  - Wiley
PY  - 2006
SN  - 1058-8388
ER  - 

TY  - JOUR
AU  - Blak, A.A.
AU  - Naserke, T.*
AU  - Vogt Weisenhorn, D.M.
AU  - Prakash, N.*
AU  - Partanen, J.*
AU  - Wurst, W.
C1  - 171
C2  - 22834
SP  - 1023-1030
TI  - Expression of Fgf receptors 1,2 and 3 in the developing mid- and hindbrain of the mouse.
JO  - Dev. Dyn.
VL  - 233
PB  - Wiley
PY  - 2005
SN  - 1058-8388
ER  - 

TY  - JOUR
AU  - Rieger, S.
AU  - Kulkarni, R.P.*
AU  - Darcy, D.*
AU  - Fraser, S.E.*
AU  - Köster, R.W.
C1  - 2453
C2  - 23500
SP  - 670-681
TI  - Quantum dots are powerful multipurpose vital labeling agents in zebrafish embryos.
JO  - Dev. Dyn.
VL  - 234
PB  - Wiley
PY  - 2005
SN  - 1058-8388
ER  - 

TY  - JOUR
AU  - Arakawa, H.
AU  - Buerstedde, J.-M.
C1  - 3628
C2  - 22229
SP  - 458-464
TI  - Immunoglobulin gene conversion: Insights from bursal B cells and the DT40 cell line.
JO  - Dev. Dyn.
VL  - 229
PB  - Wiley
PY  - 2004
SN  - 1058-8388
ER  - 

TY  - JOUR
AU  - Tallafuß, A.*
AU  - Adolf, B.*
AU  - Bally-Cuif, L.
C1  - 22281
C2  - 21061
SP  - 524-535
TI  - Selective Control of Neuronal Cluster Size at the Forebrain/Midbrain Boundary by Signaling from the Prechordal Plate.
JO  - Dev. Dyn.
VL  - 227
PB  - Wiley
PY  - 2003
SN  - 1058-8388
ER  - 

TY  - JOUR
AU  - Nacke, S.*
AU  - Schäfer, R.*
AU  - Hrabě de Angelis, M.
AU  - Mundlos, S.*
C1  - 22321
C2  - 21142
SP  - 192-200
TI  - Mouse mutant "Rib-vertebrae" (rv) : a defect in somite polarity.
JO  - Dev. Dyn.
VL  - 219
PB  - Wiley
PY  - 2000
SN  - 1058-8388
ER  - 

TY  - JOUR
AU  - Dietz, U.H.
AU  - Ziegelmeier, G.
AU  - Bittner, K.*
AU  - Bruckner, P.*
AU  - Balling, R.
C1  - 21180
C2  - 19226
SP  - 233-243
TI  - Spatio-temporal distribution of chondromodulin-I mRNA in the chicken embryo: Expression during cartilage development and formation of the heart and eye.
JO  - Dev. Dyn.
VL  - 261
PY  - 1999
SN  - 1058-8388
ER  - 

TY  - JOUR
AB  - In a retinoic acid (RA) gene trap screen of mouse embryonic stem (ES) cells, a novel gene, named Aquarius (Aqr), was identified and characterized. The promoterless lacZ marker was used to trap the genomic locus and to determine the expression pattern of the gene. Aqr transcripts are strongly induced in response to RA in vitro. During embryogenesis, Aqr is expressed in mesoderm, in the neural crest and its target tissues, and in neuroepithelium. Expression was first detected at 8.5 days postcoitum, when neural crest cells are visible at the lateral ridges of the neural plate. The gene-trapped Aqr locus was transmitted through the mouse germ line in three genetic backgrounds. In the F2 generation, the expected mendelian ratio of 1:2:1 was observed in all backgrounds, indicating that homozygous mice are viable. Homozygotes are normal in size and weight and breed normally. The gene trap insertion, however, does not seem to generate a null mutation, because Aqr transcripts are still present in the homozygous mutant animals. The Aqr open reading frame has weak homology to RNA-dependent RNA polymerases (RRPs) of the murine hepatitis viruses and contains an RRP motif. Aqr was mapped to mouse chromosome 2 between regions E5 through F2 by using fluorescence in situ hybridization analysis.
AU  - Sam, M.*
AU  - Wurst, W.
AU  - Klüppel, M.*
AU  - Jin, O.*
AU  - Heng, H.*
AU  - Bernstein, A.*
C1  - 22866
C2  - 31170
SP  - 304-317
TI  - Aquarius, a novel gene isolated by gene trapping with an RNA-dependent RNA polymerase motif.
JO  - Dev. Dyn.
VL  - 212
IS  - 2
PB  - Wiley
PY  - 1998
SN  - 1058-8388
ER  -