TY  - JOUR
AB  - The ubiquitous use of phthalates in various materials and the knowledge about their potential adverse effects is of great concern for human health. Several studies have uncovered their role in carcinogenic events and suggest various phthalate-associated adverse health effects that include pulmonary diseases. However, only limited information on pulmonary toxicity is available considering inhalation of phthalates as the route of exposure. While in vitro studies are often based on submerged exposures, this study aimed to expose A549 alveolar epithelial cells at the air-liquid interface (ALI) to unravel the genotoxic and oxidative stress-inducing potential of dibutyl phthalate (DBP) with concentrations relevant at occupational settings. Within this scope, a computer modeling approach calculating alveolar deposition of DBP particles in the human lung was used to define in vitro ALI exposure conditions comparable to potential occupational DBP exposures. The deposited mass of DBP ranged from 0.03 to 20 ng/cm2 , which was comparable to results of a human lung particle deposition model using an 8 h workplace threshold limit value of 580 μg/m3 proposed by the Scientific Committee on Occupational Exposure Limits for the European Union. Comet and Micronucleus assay revealed that DBP induced genotoxicity at DNA and chromosome level in sub-cytotoxic conditions. Since genomic instability was accompanied by increased generation of the lipid peroxidation marker malondialdehyde, oxidative stress might play an important role in phthalate-induced genotoxicity. The results highlight the importance of adapting in vitro studies to exposure scenarios relevant at occupational settings and reconsidering occupational exposure limits for DBP.
AU  - Binder, S.
AU  - Cao, X.
AU  - Bauer, S.
AU  - Rastak, N.
AU  - Kuhn, E.
AU  - Dragan, G.C.*
AU  - Monsé, C.*
AU  - Ferron, G.A.
AU  - Breuer, D.*
AU  - Oeder, S.
AU  - Karg, E.W.
AU  - Sklorz, M.
AU  - Di Bucchianico, S.
AU  - Zimmermann, R.
C1  - 63281
C2  - 51266
CY  - 111 River St, Hoboken 07030-5774, Nj Usa
TI  - In vitro genotoxicity of dibutyl phthalate on A549 lung cells at air-liquid interface in exposure concentrations relevant at workplaces.
JO  - Environ. Mol. Mutagen.
PB  - Wiley
PY  - 2021
SN  - 0192-2521
ER  - 

TY  - JOUR
AB  - no abstract
AU  - Adler, I.-D.
AU  - Favor, J.
C1  - 6241
C2  - 28863
CY  - New York, NY
SP  - 171-173
TI  - Memorial for Udo H. Ehling (1928-2010).
JO  - Environ. Mol. Mutagen.
VL  - 52
IS  - 3
PB  - Wiley-Blackwell
PY  - 2011
SN  - 0192-2521
ER  - 

TY  - JOUR
AU  - Attia, S.M.*
AU  - Kliesch, U.
AU  - Schriever-Schwemmer, G.*
AU  - Badary, O.A.*
AU  - Hamada, F.M.*
AU  - Adler, I.-D.
C1  - 22222
C2  - 20937
SP  - 99-103
TI  - Etoposide and Merbarone are Clastogenic and Aneugenic in the Mouse Bone Marrow Micronucleus Test Complemented by Fluorescence in Situ Hybridization with the Mouse minor Satellite DNA Probe.
JO  - Environ. Mol. Mutagen.
VL  - 41
PY  - 2003
SN  - 0192-2521
ER  - 

TY  - JOUR
AB  - Non-isotopic in situ hybridization using a mouse gamma (major) satellite probe DNA was applied to detect centromeres in micronuclei, which were induced in vitro in mouse liver cells by ionizing radiation and by vinblastine sulfate. In a cytokinesis-blocked micronucleus assay a dose-dependent induction of micronuclei was found for both agents. After vinblastine exposure the observed micronuclei showed centromere-positive hybridization signals in an order of magnitude of 70-90%, but after radiation exposure the magnitude was only 10-20%. Since the in situ hybridization technique detects centromeric DNA directly, it can be used in a cytokinesis-blocked micronucleus assay for a rapid and reliable discrimination between aneuploidy-inducing and clastogenic agents.
AU  - Salassidis, K.
AU  - Huber, R.
AU  - Zitzelsberger, H.
AU  - Bauchinger, M.
C1  - 19097
C2  - 12152
SP  - 1-6
TI  - Centromere Detection in Vinblastine- and Radiation-induced Micronuclei of Cytokinesis-blocked Mouse Cells Using in Situ Hybridization with a Mouse gamma (major) Satellite DNA Probe.
JO  - Environ. Mol. Mutagen.
VL  - 19
IS  - 1
PY  - 1992
SN  - 0192-2521
ER  -