TY - JOUR AB - BACKGROUND: The prognostic impact of hsa-miRNA-182-5p in oral cancer remains unexplored. Therefore, the aim of this study was to investigate the prognostic value of hsa-miRNA-182-5p and its predicted target kinectin 1 (KTN1) in oral squamous cell carcinoma (OSCC). METHOD: Expression level of hsa-miRNA-182-5p was analyzed in tumor tissue (n = 36) and healthy oral mucosal tissue (n = 17) using quantitative real-time polymerase chain reaction. Protein level of the predicted target KTN1 was detected via immunohistochemistry. Results were validated in a cohort of The Cancer Genome Atlas (TCGA). RESULTS: After dividing the data into a subgroup with high and low hsa-miRNA-182-5p expression level, a significant better overall (p = 0.016), recurrence-free (p = 0.009), and progression-free survival (p = 0.004) was observed in an upregulation of hsa-miRNA-182-5p. Staining intensity of KTN1 showed a reciprocal impact on the prognosis. Validation in a TCGA cohort confirmed these results. CONCLUSION: Our results indicate hsa-miRNA-182-5p and KTN1 as potential biomarkers for OSCC. AU - Jakob, M.* AU - Mattes, L.M.* AU - Unger, K. AU - Kueffer, S.* AU - Hess J. AU - Canis, M.* AU - Schirmer, M.* AU - Spiegel, J.L.* AU - Haubner, F.* AU - Ihler, F.* AU - Weiss, B.G.* AU - Kitz, J.* C1 - 63187 C2 - 51374 CY - 111 River St, Hoboken 07030-5774, Nj Usa SP - 3707-3719 TI - Human microRNA-182-5p and kinectin 1: Potential biomarkers for prognosis in oral squamous cell carcinoma. JO - Head Neck VL - 43 IS - 12 PB - Wiley PY - 2021 SN - 1043-3074 ER - TY - JOUR AB - BACKGROUND: MicroRNAs (miRNA) recently evolved as potential cancer biomarkers. Therefore, the aim of the present study was to evaluate the prognostic impact of eight miRNAs connected to oral squamous cell carcinoma (OSCC). METHOD: Expression levels of hsa-mir-21-5p, hsa-mir-29b-3p, hsa-mir-31-5p, hsa-mir-99a-5p, hsa-mir-99b-3p, hsa-mir-100-5p, hsa-mir-143-3p and hsa-mir-155-5p were analyzed in tumor tissue (n = 36) and healthy oral mucosal tissue (n = 17) and correlated with clinical variables. Results of the study cohort were validated in an OSCC cohort of The Cancer Genome Atlas. RESULTS: Increased hsa-mir-99b-3p expression level showed a tendency toward advanced tumor stages, and high levels of hsa-mir-100-5p expression were associated with extracapsular extension. While a high expression level of hsa-mir-99b-3p was associated with better survival, a high expression level of hsa-mir-100-5p was correlated with a poorer survival in the study cohort. CONCLUSION: Our results indicate that hsa-mir-99b-3p and hsa-mir-100-5p may serve as novel prognostic biomarkers in OSCC. AU - Jakob, M.* AU - Mattes, L.* AU - Küffer, S.* AU - Unger, K. AU - Hess J. AU - Bertlich, M.* AU - Haubner, F.* AU - Ihler, F.* AU - Canis, M.* AU - Weiss, B.* AU - Kitz, J.* C1 - 56736 C2 - 47229 SP - 3499-3515 TI - MicroRNA expression patterns in oral squamous cell carcinoma: Hsa-mir-99b-3p and hsa-mir-100-5p as novel prognostic markers for oral cancer. JO - Head Neck VL - 41 IS - 10 PY - 2019 SN - 1043-3074 ER - TY - JOUR AB - Background: The surgeon's evaluation of resection status based on frozen section analysis during operation and pathological examination of resected specimens often differ. For this study, we recapitulated the surgeon's perspective during an operation, accordingly classified the evaluation of margins by the surgeon, and analyzed its impact on the outcome compared with the pathological results. Methods: This was a retrospective analysis. As data sources, paper-based and digital patient files, as well as the Munich Cancer Registry database were used. Results: Three hundred ninety-six cases were included in this analysis. Only the evaluation of margins by the surgeon influenced local control, and the pathological results influenced disease-free survival (DFS). Surprisingly, margins of >5 mm of normal tissue to cancer growth led to local control and overall survival (OS) significantly worse than 1 to 5-mm resections. Conclusion: The evaluation of margins by the surgeon is of significant importance for local control and OS. It is largely based on frozen section analysis, which, therefore, should be used whenever possible. AU - Baumeister, P. AU - Baumüller, K.* AU - Harréus, U.* AU - Reiter, M.* AU - Welz, C.* C1 - 53892 C2 - 45122 SP - 963-972 TI - Evaluation of margins in head and neck squamous cell carcinoma from the surgeon's perspective. JO - Head Neck VL - 40 IS - 5 PY - 2018 SN - 1043-3074 ER - TY - JOUR AB - Background: Comorbidity and anemia strongly influence treatment and outcome of oropharyngeal squamous cell carcinomas (SCCs), but respective data are frequently lacking in clinical studies. We evaluated 149 cases of oropharyngeal SCC primarily treated with surgery regarding the impact of these factors on disease-free survival (DFS) and overall survival (OS). Methods: Data were recorded during treatment and follow-up visits. Human papillomavirus (HPV) association of oropharyngeal SCC was detected by p16 immunohistochemistry. Results: Patients with p16-positive disease were significantly less comorbid and anemic, and treated more aggressively. Comorbidity and anemia, but not p16 status, significantly influenced DFS and had more impact on OS than p16 status. Conclusion: In our study, p16-positive seems a good surrogate marker for healthier patients. Patients with p16-positive disease showed improved survival, but this was limited to none/mild comorbid and/or nonanemic individuals in this group. On the basis of our results, we strongly suggest the inclusion of these factors in risk stratification for clinical studies. AU - Baumeister, P. AU - Rauch, J.* AU - Jacobi, C.* AU - Kisser, U.* AU - Betz, C.* AU - Becker, S.* AU - Reiter, M.* C1 - 49125 C2 - 41656 SP - 7-16 TI - Impact of comorbidity and anemia in patients with oropharyngeal cancer primarily treated with surgery in the human papillomavirus era. JO - Head Neck VL - 39 IS - 1 PY - 2017 SN - 1043-3074 ER -