TY  - JOUR
AB  - PURPOSE: For women with locoregionally advanced cervical cancer, the standard of care treatment is the curatively intended chemoradiation therapy (CRT). A relationship between bone marrow (BM) dose-volume histograms (DVHs) and acute hematological toxicity (HT) has been debated recently. Aim of this study was the evaluation of BM dose constraints and HT in a contemporary patient cohort. METHODS: Radiation treatment plans of 31 patients with cervical cancer (FIGO stage IIB-IVB) treated with intensity-modulated radiotherapy and simultaneous chemotherapy were explored retrospective. Pelvic bones (PB) and femoral heads (FH) were contoured and DVHs were correlated with white blood cells (WBC), hemoglobin levels and platelets. RESULTS: Comparing the absolute blood levels with the dose volumes of both FH and PB the data showed a significant correlation between WBC and the median dose of the FH and the median dose, V30Gy, V40Gy and V50Gy of the PB. A correlation between the toxicity grade of anemia and mean dose, maximum dose and V5Gy of the PB was found. Counting the highest grade of HT of all three blood levels of each patient, significant correlations were found for the mean and median dose, V30Gy, V40Gy and V50Gy of the PB. CONCLUSION: The results show that blood levels may correlate with distinct dosimetric subvolumes of critical bone marrow compartments with a potential impact on therapeutic outcome and treatment-related toxicity. The data presented are in line with the previous findings on the relevance of dosimetric exposure of pelvic bony subvolumes.
AU  - Hallqvist, D.*
AU  - Kormann, C.*
AU  - Pigorsch, S.*
AU  - Kiechle, M.*
AU  - Combs, S.E.
AU  - Habermehl, D.*
C1  - 71569
C2  - 56281
CY  - Campus, 4 Crinan St, London N1 9xw, England
TI  - Bone marrow toxicity in patients with locally advanced cervical cancer undergoing multimodal treatment with VMAT/IMRT: Are there dosimetric predictors for toxicity?
JO  - Eur. J. Med. Res.
VL  - 29
IS  - 1
PB  - Bmc
PY  - 2024
SN  - 0949-2321
ER  - 

TY  - JOUR
AB  - Background: An effective testing strategy is essential for pandemic control of the novel Coronavirus disease 2019 (COVID-19) caused by infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Breath gas analysis can expand the available toolbox for diagnostic tests by using a rapid, cost-beneficial, high-throughput point-of-care test. We conducted a bi-center clinical pilot study in Germany to evaluate breath gas analysis using multi-capillary column ion mobility spectrometry (MCC-IMS) to detect SARS-CoV-2 infection. Methods: Between September 23, 2020, and June 11, 2021, breath gas measurements were performed on 380 patients (SARS-CoV-2 real-time polymerase chain reaction (PCR) positive: 186; PCR negative: 194) presenting to the emergency department (ED) with respiratory symptoms. Results: Breath gas analysis using MCC-IMS identified 110 peaks; 54 showed statistically significant differences in peak intensity between the SARS-CoV-2 PCR-negative and PCR-positive groups. A decision tree analysis classification resulted in a sensitivity of 83% and specificity of 86%, but limited robustness to dataset changes. Modest values for the sensitivity (74%) and specificity (52%) were obtained using linear discriminant analysis. A systematic search for peaks led to a sensitivity of 77% and specificity of 67%; however, validation by transferability to other data is questionable. Conclusions: Despite identifying several peaks by MCC-IMS with significant differences in peak intensity between PCR-negative and PCR-positive samples, finding a classification system that allows reliable differentiation between the two groups proved to be difficult. However, with some modifications to the setup, breath gas analysis using MCC-IMS may be a useful diagnostic toolbox for SARS-CoV-2 infection. Trial registration: This study was registered at ClinicalTrials.gov on September 21, 2020 (NCT04556318; Study-ID: HC-N-H-2004).
AU  - Voit, F.*
AU  - Erber, J.*
AU  - Feuerherd, M.
AU  - Fries, H.*
AU  - Bitterlich, N.*
AU  - Diehl-Wiesenecker, E.*
AU  - Gladis, S.*
AU  - Lieb, J.*
AU  - Protzer, U.
AU  - Schneider, J.*
AU  - Geisler, F.*
AU  - Somasundaram, R.*
AU  - Schmid, R.M.*
AU  - Bauer, W.*
AU  - Spinner, C.D.*
C1  - 68335
C2  - 54752
CY  - Campus, 4 Crinan St, London N1 9xw, England
TI  - Rapid point-of-care detection of SARS-CoV-2 infection in exhaled breath using ion mobility spectrometry: A pilot study.
JO  - Eur. J. Med. Res.
VL  - 28
IS  - 1
PB  - Bmc
PY  - 2023
SN  - 0949-2321
ER  - 

TY  - JOUR
AB  - Skin affections after sulfur mustard (SM) exposure include erythema, blister formation and severe inflammation. An antidote or specific therapy does not exist. Anti-inflammatory compounds as well as substances counteracting SM-induced cell death are under investigation. In this study, we investigated the benzylisoquinoline alkaloide berberine (BER), a metabolite in plants like berberis vulgaris, which is used as herbal pharmaceutical in Asian countries, against SM toxicity using a well-established in vitro approach. Keratinocyte (HaCaT) mono-cultures (MoC) or HaCaT/THP-1 co-cultures (CoC) were challenged with 100, 200 or 300 mM SM for 1 h. Post-exposure, both MoC and CoC were treated with 10, 30 or 50 mu M BER for 24 h. At that time, supernatants were collected and analyzed both for interleukine (IL) 6 and 8 levels and for content of adenylate-kinase (AK) as surrogate marker for cell necrosis. Cells were lysed and nucleosome formation as marker for late apoptosis was assessed. In parallel, AK in cells was determined for normalization purposes. BER treatment did not influence necrosis, but significantly decreased apoptosis. Anti-inflammatory effects were moderate, but also significant, primarily in CoC. Overall, BER has protective effects against SM toxicity in vitro. Whether this holds true should be evaluated in future in vivo studies.
AU  - Poh, P.S.P.*
AU  - Schmauss, V.*
AU  - McGovern, J.A.*
AU  - Schmauss, D.*
AU  - Chhaya, M.P.*
AU  - Foehr, P.*
AU  - Seeger, M.*
AU  - Ntziachristos, V.
AU  - Hutmacher, D.W.*
AU  - van Griensven, M.*
AU  - Schantz, J.*
AU  - Balmayor, E.R.*
C1  - 53737
C2  - 44966
CY  - Elsevier House, Brookvale Plaza, East Park Shannon, Co, Clare, 00000, Ireland
TI  - Non-linear optical microscopy and histological analysis of collagen, elastin and lysyl oxidase expression in breast capsular contracture.
JO  - Eur. J. Med. Res.
VL  - 23
IS  - 1
PB  - Elsevier Ireland Ltd
PY  - 2018
SN  - 0949-2321
ER  - 

TY  - JOUR
AB  - Purpose: In elderly patients with esophageal cancer (EC), esophagectomy is associated with an increased mortality, and therefore these patients are often treated with definite (chemo) radiation. The purpose of this study was to assess the toxicity and efficiency of definite radio(chemo) therapy in patients >75 years compared with definite radio(chemo) therapy in patients <75 years. Methods: 32 patients >75 years were treated with definite radio(chemo) therapy for EC. We compared baseline parameters, efficiency and toxicity rates of these patients to 39 patients <75 years. Results: Patients <75 years were more likely to receive simultaneous chemotherapy, and had a lower age-adjusted Charlson comorbidity index (ACCI). 25% of elderly patients were treated in palliative intent. There was no significant difference in progression-free survival between patient groups. No significant differences were seen for overall survival (15.7 months vs. 19.9 months; p = 0.102) and progression-free survival (10.5 months vs. 9.2 months, p = 0.470) between older patients treated with curative intent and younger patients. In addition, there were no significant differences for dysphagia and hematological side effects between elderly patients and younger patients. Conclusion: Definite (chemo) radiation is a feasible therapy for elderly patients. OS and PFS in elderly patients with a curative treatment approach are comparable to younger patients and it is not associated with higher toxicity rates.
AU  - Muench, S.*
AU  - Heinrich, C.*
AU  - Habermehl, D.*
AU  - Oechsner, M.*
AU  - Combs, S.E.
AU  - Duma, M.N.
C1  - 51596
C2  - 43269
CY  - London
TI  - Primary radio(chemo)therapy for esophageal cancer in elderly patients: Are efficiency and toxicity comparable with younger patients?
JO  - Eur. J. Med. Res.
VL  - 22
IS  - 1
PB  - Biomed Central Ltd
PY  - 2017
SN  - 0949-2321
ER  - 

TY  - JOUR
AB  - The interaction between implant material and surrounding tissues is believed to play a fundamental role in implant success. Although bone sialoprotein (BSP) has been found to be osteoinductive when coated onto femoral implants, collagen and fibronectin are the most used compounds for preparation of pre-coated cell culture slides at present. In this study, the support of BSP-, collagen- and fibronectin-coated and non-coated implant material for the development of adult human maxillar bone in vitro was studied and compared. The expression of bone turnover markers like BSP and osteocalcin as well as osteonectin, transforming growth factor beta (TGF-beta) and CD90 during different time periods of cell cultivation (3, 5, 10, 15, 20 and 25 days) was visualized immunohistochemically. The distribution patterns of the cells were examined on a rough surface of the titanium-hydroxyapatite dental implant material TICER and on a total smooth surface of the technical implant material glimmer. Significantly different values were found for glimmer at the 15. and the 20. Div, exclusively, indicating that a smooth surface was more improved than a rough ceramic surface by pre-coatings. The White-test using rankings of the median values gave evidence for BSP-coatings at position 1 followed by collagen. Our experiments were designed to use very low concentrated BSP coating solution with the aim to reduce the healing time with a minimal effort and minimal risks for the patients.
AU  - Hilbig, H.*
AU  - Kirsten, M.*
AU  - Rupietta, R.*
AU  - Graf, H.L.*
AU  - Thalhammer, S.
AU  - Strasser, S.*
AU  - Armbruster, F.P.*
C1  - 2289
C2  - 24986
SP  - 6-12
TI  - Implant surface coatings with bone sialoprotein, collagen, and fibronectin and their effects on cells derived from human maxillar bone.
JO  - Eur. J. Med. Res.
VL  - 12
IS  - 1
PB  - Med. Scientific Publ. Holzapfel
PY  - 2007
SN  - 0949-2321
ER  - 

TY  - JOUR
AU  - Kalies, H.*
AU  - Heinrich, J.
AU  - Borte, M.*
AU  - Schaaf, B.*
AU  - von Berg, A.*
AU  - von Kries, R.*
AU  - Wichmann, H.-E.
AU  - Bolte, G.
C1  - 4719
C2  - 22681
SP  - 36-42
TI  - The effect of breastfeeding on weight gain in infants: Results of a birth cohort study.
JO  - Eur. J. Med. Res.
VL  - 10
PY  - 2005
SN  - 0949-2321
ER  - 

TY  - JOUR
AU  - Seemann, M.D.*
AU  - Schmid, F.*
AU  - Englmeier, K.-H.
C1  - 938
C2  - 22755
SP  - 195-196
TI  - Hybrid rendering of aortic aneurysms after endoluminal stent-graft repair.
JO  - Eur. J. Med. Res.
VL  - 10
PY  - 2005
SN  - 0949-2321
ER  - 

TY  - JOUR
AU  - Englmeier, K.-H.
AU  - Schmid, K.
AU  - Hildebrand, C.
AU  - Bichler, S.
AU  - Porta, M.*
AU  - Maurino, M.*
AU  - Bek, T.*
C1  - 2502
C2  - 22151
SP  - 473-478
TI  - Early detection of diabetes retinopathy by new algorithms for automatic recognition of vascular changes.
JO  - Eur. J. Med. Res.
VL  - 9
PY  - 2004
SN  - 0949-2321
ER  - 

TY  - JOUR
AU  - Kompauer, I.
AU  - Demmelmair, H.*
AU  - Koletzko, B.*
AU  - Bolte, G.*
AU  - Linseisen, J.*
AU  - Heinrich, J.
C1  - 3566
C2  - 22183
SP  - 378-382
TI  - n6/n3 hypothesis and allergies: Biologically plausible, but not confirmed.
JO  - Eur. J. Med. Res.
VL  - 9
PY  - 2004
SN  - 0949-2321
ER  - 

TY  - JOUR
AU  - Eife, R.*
AU  - Weiss, M.*
AU  - Barros, V.*
AU  - Sigmund, B.*
AU  - Goriup, U.*
AU  - Komb, D.*
AU  - Wolf, W.*
AU  - Kittel, J.*
AU  - Schramel, P.
AU  - Reiter, K.*
C1  - 21122
C2  - 19160
SP  - 219-223
TI  - Chronic poisoning by copper in tap water. I: Copper intoxications with predominantly gastointestinal symtoms.
JO  - Eur. J. Med. Res.
VL  - 4
PY  - 1999
SN  - 0949-2321
ER  - 

TY  - JOUR
AU  - Eife,R.*
AU  - Müller-Höcker, J.*
AU  - Lang, T.*
AU  - Barros, V.*
AU  - Sigmund, B.*
AU  - Thanner, F.*
AU  - Welling, P.*
AU  - Lange, H.*
AU  - Wolf, W.*
AU  - Rodeck, B.*
AU  - Kittel, J.*
AU  - Schramel, P.
AU  - Reiter, K.*
C1  - 21121
C2  - 19159
SP  - 224-228
TI  - Chronic poisoning by copper in tap water. II: Copper intoxications with predominantly systemic symptoms.
JO  - Eur. J. Med. Res.
VL  - 4
PY  - 1999
SN  - 0949-2321
ER  - 

TY  - JOUR
AU  - Heinrich, J.
AU  - Hoelscher, B.
AU  - Jacob, B.
AU  - Wjst, M.
AU  - Wichmann, H.-E.
C1  - 21102
C2  - 19135
SP  - 107-113
TI  - Trends in allergies among children in a region of former East Germany between 1992-1993 and 1995-1996.
JO  - Eur. J. Med. Res.
VL  - 4
PY  - 1999
SN  - 0949-2321
ER  - 

TY  - JOUR
AU  - Seemann, M.D.*
AU  - Seemann, O.*
AU  - Englmeier, K.-H.
AU  - Allen, C.M.*
AU  - Haubner, M.
AU  - Reiser, M.F.*
C1  - 21197
C2  - 19279
SP  - 515-522
TI  - Hybrid rendering and virtual endoscopy of the auditory and vestibular system.
JO  - Eur. J. Med. Res.
VL  - 3
PY  - 1998
SN  - 0949-2321
ER  - 

TY  - JOUR
AB  - Surface active material potentially present in the airway is difficult to analyse due to the tight binding of surfactant components to mucins. A surface active sol-fraction was obtained from sputum of patients with cystic fibrosis (CF), analysed and compared with the sol-fraction from sputum of tracheomized, non-CF patients. The release of phospholipids from CF sputum was relatively fast being completed within minutes, temperature dependent and averaged 5.6 +/- 2.2% of total phospholipid mass. In comparison to sputum, the phospholipid composition of the sol fraction was the same except for a lower percentage of phosphatidylethanolamine, which is usually found primarily cell membrane associated. The sol-fraction from the CF patient group had a lower percentage of phosphatidylcholine and about 3 times more surfactant protein A than that from the non-CF patients. Surface activity did not differ between CF and non-CF samples. Of interest, the adsorption rate (gamma ads, about 30-35 mN/m) and the minimal surface tension (gamma min, about 20-25 mN/m) were relatively low. These data support the hypothesis that surface active material can be released from sputum and that it might support its transport by reducing mucus adhesiveness to the airways.
AU  - Griese, M.*
AU  - Duroux, A.*
AU  - Schams, A.*
AU  - Lenz, A.-G.
AU  - Kleinsasser, N.*
C1  - 28748
C2  - 33543
SP  - 114-120
TI  - Tracheobronchial surface active material in cystic fibrosis.
JO  - Eur. J. Med. Res.
VL  - 2
IS  - 3
PY  - 1997
SN  - 0949-2321
ER  - 

TY  - JOUR
AB  - It has been reported that females show an increased frequency of bronchial hyperresponsiveness (BHR) compared to males and that this difference is abolished after taking into account differences in baseline FEV1. The aim of our study was to analyse how the distribution of BHR in males and females depends on the definition of BHR. Special emphasis was paid to the question whether the prevalence rates of BHR according to different definitions were related to baseline characteristics of the subjects and baseline lung function in the same manner. We analysed the data obtained within the European Community Respiratory Health Survey (ECRHS) in the Eastern German population sample of Erfurt aged 20-65 years (n = 931). In logistic regression analyses of different definitions of BHR, we used as parameters age, height, gender, smoking habits, baseline forced expiratory volume in one second (FEV1), forced vital capacity (FVC), and FEV1 as a percent of FVC (FEV1% FVC). Symptoms and reported diagnosis of asthma did not significantly depend on gender or age. When BHR was defined as the provocative dose causing a 20% fall in FEV1, BHR was more prevalent in females than in males (27.6% vs. 13.2%). Similar gender differences were found when defining BHR via a 10% fall in FEV1 or by using corresponding cut-off values of the linear dose-response slopes of the percent decline in FEV1 (DRS). Multiple linear regression analyses of various transformations of the DRS also indicated a higher degree of BHR in females. Independently of the definition chosen, however, the gender difference in the prevalence of BHR disappeared when height and FEV1 or FEV1% FVC or appropriate combinations were included in the model. The reciprocally transformed DRS showed the best resolution of the spectrum of bronchial responsiveness. These data are compatible with the hypotheses that (1) estimates of the distribution of BHR are distorted by differences in the methacholine dosage per lung size and that (2) airway geometry affects the measurement of BHR. It appears that these factors and not intrinsic differences in BHR between males and females contribute to the gender differences in the prevalence of BHR. Furthermore, our data support the superiority of the dose response slope for the analysis of bronchial responsiveness in epidemiologic surveys.
AU  - Wassmer, G.*
AU  - Jörres, R.A.*
AU  - Heinrich, J.*
AU  - Wjst, M.*
AU  - Reitmeir, P.*
AU  - Wichmann, H.-E.
C1  - 24190
C2  - 31462
SP  - 47-54
TI  - The association between baseline lung function and bronchial responsiveness to methacholine.
JO  - Eur. J. Med. Res.
VL  - 2
IS  - 2
PB  - BioMed Central
PY  - 1997
SN  - 0949-2321
ER  -