TY - JOUR AU - Amarie, O.V. C1 - 59874 C2 - 49082 CY - 111 River St, Hoboken 07030-5774, Nj Usa TI - Retinal degeneration development in Cnga1 deficient mouse. JO - Acta Ophthalmol. VL - 97 PB - Wiley PY - 2019 SN - 1755-375X ER - TY - JOUR AU - Graw, J. C1 - 59875 C2 - 49083 CY - 111 River St, Hoboken 07030-5774, Nj Usa TI - Mutation in the mouse histone gene Hist2h3c1 leads to degeneration of the lens vesicle and severe microphthalmia. JO - Acta Ophthalmol. VL - 97 PB - Wiley PY - 2019 SN - 1755-375X ER - TY - JOUR AU - Michalke, B. C1 - 59871 C2 - 49081 CY - 111 River St, Hoboken 07030-5774, Nj Usa TI - Trace elements in glaucoma - The impact of analytics. JO - Acta Ophthalmol. VL - 97 PB - Wiley PY - 2019 SN - 1755-375X ER - TY - JOUR AU - Pawliczek, D. C1 - 59873 C2 - 49074 CY - 111 River St, Hoboken 07030-5774, Nj Usa TI - Cataract type in mouse exposed to moderate doses of ionizing radiation depends on mouse age at irradiation. JO - Acta Ophthalmol. VL - 97 PB - Wiley PY - 2019 SN - 1755-375X ER - TY - JOUR AU - Amarie, O.V. AU - Fuchs, H. AU - Gailus-Durner, V. AU - Hrabě de Angelis, M. AU - Graw, J. C1 - 55364 C2 - 46154 CY - 111 River St, Hoboken 07030-5774, Nj Usa SP - 4-4 TI - Animal case. JO - Acta Ophthalmol. VL - 96 PB - Wiley PY - 2018 SN - 1755-375X ER - TY - JOUR AU - Kamme, C.* AU - Mayer, A.K.* AU - Strom, T.M. AU - Andreasson, S.* AU - Weisschuh, N.* C1 - 50194 C2 - 42239 CY - Hoboken SP - E250-E252 TI - Genotype and phenotype in an unusual form of Laurence-Moon-Bardet-Biedl syndrome. JO - Acta Ophthalmol. VL - 95 IS - 3 PB - Wiley PY - 2016 SN - 1755-375X ER - TY - JOUR AB - Purpose: To determine the structural characteristics of lens epithelial cells (LECs) found on the anterior portion of the lens capsule and their pluripotency, proliferating and migrating potential when grown ex vivo with relevance to posterior capsular opacification (PCO) after cataract surgery. Methods: The explants of anterior portion of the lens capsule consisting of monolayer of LECs were obtained from uneventful cataract surgery and were cultivated under adherent conditions. The size and shape of the outgrowing cells were recorded by scanning electron microscopy (SEM), while their migration and proliferation potential were followed using light microscopy. Positivity for proliferation (Ki-67)- and pluripotency (Sox2)-specific markers were tested by immunofluorescent staining. Results: The proliferation and migration of anterior portion of the lens capsule's LECs filling up the denuded and reverse side regions of the lens capsule as well as their growth on glass culture surfaces could be followed by light microscopy and SEM, while the distribution of LECs and their morphology could be analysed in detail by SEM. The expression of Ki-67 and Sox2 in LECs growing adherently on human anterior portion of the lens capsule could also be detected. Conclusions: Classic light microscopy and SEM can be used to show that human anterior portion of the lens capsule harbours LECs that can proliferate and migrate, suggesting their pluripotency or putative stem cell nature. Similarly, morphological techniques can be used to study PCO and the effect different drugs or physical treatments have against PCO development. AU - Andjelić, S.* AU - Drašlar, K.* AU - Lumi, X.* AU - Yan, X. AU - Graw, J. AU - Facskó, A.* AU - Hawlina, M.* AU - Petrovski, G.* C1 - 43228 C2 - 36332 SP - e499–e506 TI - Morphological and proliferative studies on ex vivo cultured human anterior lens epithelial cells - relevance to capsular opacification. JO - Acta Ophthalmol. VL - 93 IS - 6 PY - 2015 SN - 1755-375X ER - TY - JOUR AB - Purpose To estimate the prevalence of major age-related eye diseases in a population-based regional study in Southern Germany. Methods 822 randomly selected persons (age 68-96 years) from the KORA AGE study were asked in 2012 in a standardized interview for the presence of major eye disorders like cataracts, glaucoma and age-related macula degeneration (AMD). In case of any positive reply, the ophthalmologist in charge was asked for validation and specification of any eye disease. Results 465 persons reported any eye disorder (57%); 71% of them could be validated and specified. There were 182 confirmed cases of cataracts, 7 of glaucoma and 5 of AMD. Additionally, there were 52 cases of cataracts and AMD, also 54 cases of cataract and glaucoma and 11 cases of cataract, glaucoma and AMD. In 62% cases cataracts developed prior to any of the other eye diseases. Adjusted for age, women had a significantly higher risk for cataracts (OR = 1.72) and for AMD (OR = 1.94) than men; no gender-specific difference was observed for glaucoma. Among patients with cataracts, 69% had lens surgery. Conclusion We confirmed cataracts as the major age-related eye diseases; however, the number of glaucoma and AMD were surprisingly low. Further analyses are planned to identify risk factors and to show how eye diseases are independent risk factors for increased frailty and disability in the aged. This study was supported by the German Federal Ministry of Education and Research (BMBF) within the programme "Healthy Ageing" (FKZ 01ET1003).   AU - Graw, J. AU - Strobl, R.* AU - Heier, M. AU - Linkohr, B. AU - Peters, A. AU - Holle, R. AU - Grill, E.* C1 - 31656 C2 - 34609 CY - Hoboken SP - DOI: 10.1111/j.1755-3768.2013.46 TI - The KORA-AGE eye study: Eye diseases in the elderly. JO - Acta Ophthalmol. VL - 91 PB - Wiley-Blackwell PY - 2013 SN - 1755-375X ER - TY - JOUR AB - Purpose To summarize GMC - Eye Screen findings with about 350 tested mouse mutant lines. Methods Eye morphology was analyzed by Slit Lamp Biomicroscopy/Scheimpflug Imaging and Ophthalmoscopy/Optical Coherence Tomography, eye size by Laser Interference Biometry, and the Virtual Optokinetic Drum was used for testing visual ability. Results The GMC is a large-scale phenotyping center where mouse models for human diseases are analyzed in a standardized way. More than 550 parameters are investigated in 14 different areas including behavior, neurology, cardiovascular functionality and vision and eye development. In the past 12 years, we identified eye pathologies in 43 mutant lines of the GMC workflow. The majority of them exhibited irregular eye sizes (72%), followed by morphological changes as corneal and lens opacities (21%) or retinal damages (16%) and moderately reduced visual abilities (7%). Overall phenotype predictions (EuroPhenome database; http://www.europhenome.org/) indicated that 89% of these lines were additionally affected in behavioral and/or neurological parameters, pointing to general relations between eye and brain development. Moreover, 46% showed heart impairments, connecting eye diseases with cardiovascular risks. Our data further proved for 11 gene products a previously unknown participation in ocular processes. These include the chromatin modifiers Asxl1 and Mysm1, the transcription factor Nfya as well as Arvcf, Jmjd5 and Slc20a2 with a role in cell-cell communication, protein hydroxylation and phosphate transport, respectively. Conclusion The GMC - Eye Screen is an effective approach for improving knowledge about ocular genetics and detecting associations with non-ocular disorders.   AU - Puk, O. AU - Fuchs, H. AU - Gailus-Durner, V. AU - Hrabě de Angelis, M. AU - Graw, J. C1 - 31657 C2 - 34608 CY - Hoboken TI - The eye screen of the German Mouse Clinic (GMC) - new genetic insights into eye development and ocular disorders. JO - Acta Ophthalmol. VL - 91 PB - Wiley-Blackwell PY - 2013 SN - 1755-375X ER -