TY  - JOUR
AB  - BACKGROUND: To assess factors associated with the preferred role of the attending ophthalmologist in the decision-making processes before treating diabetic retinopathy (DR). METHODS: Cross-sectional study of 810 adults attending secondary diabetes care centers (NCT02311504). Diabetes patients were classified using a validated questionnaire in an ophthalmologist-dominant decision-making (ODM), shared decision-making (SDM) and patient-dominant decision-making (PDM) style. Multivariate logistic regression was performed to determine factors associated with the decision-making process. RESULTS: A majority of 74.3% patients preferred SDM between ophthalmologist and patient, 17.4% patients wanted ODM, delegating the decision-making process to the ophthalmologist, 8.3% preferred the autonomous style of PDM. Patients wanting ODM were older (OR = 1.2 per decade, p = 0.013), had a lower level of education (OR = 1.4, p = 0.001) and had a higher frequency of consultations per year (OR = 1.3, p = 0.022). Patients with better basic knowledge in DR and memorizing their HbA1c level showed a higher propensity for SDM (OR = 1.1, p = 0.037). Patients wanting PDM had a significantly higher education (OR = 1.3, p = 0.036) and a greater desire for receiving information from self-help groups (OR = 1.3, p = 0.015). CONCLUSIONS: The first evaluation of the general patient wishes for the treatment of DR confirmed the concept of SDM, which was favored by three quarters. In particular, older patients with low educational attainment wanted to delegate the decision-making process to the ophthalmologist. Amelioration of ophthalmologic education in diabetic programs might take up patients' propensity for SDM. Regardless of the decision-making group, nearly all patients wanted the medical and scientific information to be transferred by and shared with the ophthalmologist. TRIAL REGISTRATION: The study was registered on www.clinicaltrials.gov (identifier: NCT02311504) on December 4th 2014.
AU  - Marahrens, L.*
AU  - Kern, R.*
AU  - Ziemssen, T.*
AU  - Fritsche, A.
AU  - Martus, P.*
AU  - Ziemssen, F.*
AU  - Roeck, D.*
C1  - 51706
C2  - 43410
CY  - London
TI  - Patients' preferences for involvement in the decision-making process for treating diabetic retinopathy.
JO  - BMC Ophthalmol.
VL  - 17
IS  - 1
PB  - Biomed Central Ltd
PY  - 2017
SN  - 1471-2415
ER  - 

TY  - JOUR
AB  - BACKGROUND: Characterization of the neuro-glial profile of cells growing out of human idiopathic epiretinal membranes (iERMs) and testing their proliferative and pluripotent properties ex vivo is needed to better understand the pathogenesis of their formation. METHODS: iERMs obtained during uneventful vitrectomies were cultivated ex vivo under adherent conditions and assessed by standard morphological and immunocytochemical methods. The intracellular calcium dynamics of the outgrowing cells was assessed by fluorescent dye Fura-2 in response to acetylcholine (ACh)- or mechano- stimulation. RESULTS: The cells from the iERMs formed sphere-like structures when cultured ex vivo. The diameter of the spheres increased by 5% at day 6 and kept an increasing tendency over a month time. The outgrowing cells from the iERM spheres had mainly glial- and some neuronal- like morphology. ACh- or mechano- stimulation of these cells induced intracellular calcium propagation in both cell types; in the neuronal-like cells resembling action potential from the soma to the dendrites. Immunocytochemistry confirmed presence of glial- and neuronal cell phenotype (GFAP and Nestin-1 positivity, respectively) in the iERMs, as well as presence of pluripotency marker (Sox2). CONCLUSION: iERMs contain cells of neuronal- and glial- like origin which have proliferative and pluripotent potential, show functionality reflected through calcium dynamics upon ACh and mechano- stimulation, and a corresponding molecular phenotype.
AU  - Andjeli, S.*
AU  - Lumi, X.*
AU  - Yan, X.
AU  - Graw, J.
AU  - Moe, M.C.*
AU  - Facskó, A.*
AU  - Hawlina, M.*
AU  - Petrovski, G.*
C1  - 43067
C2  - 35979
CY  - London
TI  - Characterization of ex vivo cultured neuronal- and glial- like cells from human idiopathic epiretinal membranes.
JO  - BMC Ophthalmol.
VL  - 14
IS  - 1
PB  - Biomed Central Ltd
PY  - 2014
SN  - 1471-2415
ER  -