TY  - JOUR
AB  - Non-alcoholic fatty liver disease (NAFLD) begins with lipid accumulation and progresses toward inflammation and fibrosis. Nuclear receptors (NRs), like the Peroxisome Proliferator-Activated Receptors alpha and gamma (PPARα and PPARy), the Farnesoid X Receptor (FXR), and the Liver X receptor (LXR), regulate genes by heterodimerizing with Retinoid X receptor (RXR). These receptors are emerging targets for pharmaceutical intervention for metabolic diseases.
AU  - Schweiger, M.
AU  - Arredondo-Lasso, M.N.
AU  - Friano, M.E.
AU  - Gil Lozano, M.
AU  - Herzig, S.
AU  - Uhlenhaut, N.H.
C1  - 69989
C2  - 55353
CY  - 111 River St, Hoboken 07030-5774, Nj Usa
TI  - Lipid sensing nuclear receptors involved in the pathogenesis of fatty liver disease.
JO  - FEBS Lett.
PB  - Wiley
PY  - 2024
SN  - 0014-5793
ER  - 

TY  - JOUR
AB  - Chromophore-bearing proteins that are (reversibly) altered after light illumination are major functional components of nature. They gained considerable attention in the last decades since the dynamic interactions of the chromophore and protein matrix can be used to control downstream effects altering the functionality of proteins, cells, or complete organisms with light (optogenetics). Additionally, the photophysical effects can be employed to add capabilities to optical imaging. For example, light can be used to reversibly switch the signal on or off (e.g., fluorescence). In this article, we review chromophore and protein matrix interactions, focusing on photoswitching fluorescent proteins of the GFP family (RSFPs) and natively photoswitching bacteriophytochromes (BphPs). This review aims to provide an in-depth understanding of the dynamic interplay between photoswitching photophysics and the protein matrix and a thorough discussion on how this connection has been harnessed for the development of optogenetic and imaging tools.
AU  - Rodrigues, E.M.
AU  - Stiel, A.-C.
C1  - 67727
C2  - 54035
CY  - 111 River St, Hoboken 07030-5774, Nj Usa
SP  - 1319-1344
TI  - It's a two-way street: Photoswitching and reversible changes of the protein matrix in photoswitchable fluorescent proteins and bacteriophytochromes.
JO  - FEBS Lett.
VL  - 597
IS  - 10
PB  - Wiley
PY  - 2023
SN  - 0014-5793
ER  - 

TY  - JOUR
AB  - Adipose tissue regulates whole-body energy homeostasis. Both lipodystrophy and obesity, the extreme and opposite aspects of adipose tissue dysfunction, result in metabolic disorders: insulin resistance and hepatic steatosis. Cyclin-dependent kinases (CDKs) have been reported to be involved in adipose tissue development and functions. Using adipose tissue-specific knockout mice, here we demonstrate that the deletion of CDK7 in adipose tissue results in progressive lipodystrophy, insulin resistance, impaired adipokine secretion and down-regulation of fat-specific genes, which are aggravated on high-fat diet and during aging. Our studies suggest that CDK7 is a key regulatory component of adipose tissue maintenance and systemic energy homeostasis.
AU  - Chen, Y.*
AU  - Fernandez, E.A.*
AU  - Röger, C.*
AU  - Lopez-Mejia, I.C.*
AU  - Fajas Coll, L.*
AU  - Ji, H.
C1  - 64638
C2  - 52365
SP  - 1434-1444
TI  - Adipocyte-specific CDK7 ablation leads to progressive loss of adipose tissue and metabolic dysfunction.
JO  - FEBS Lett.
VL  - 596
IS  - 11
PY  - 2022
SN  - 0014-5793
ER  - 

TY  - JOUR
AB  - Glucocorticoids (GCs) are widely used therapeutic agents to treat a broad range of inflammatory conditions. Their functional effects are elicited by binding to the glucocorticoid receptor (GR), which regulates transcription of distinct gene networks in response to ligand. However, the mechanisms governing various aspects of undesired side effects versus beneficial immunomodulation upon GR activation remain complex and incompletely understood. In this review, we discuss emerging models of inflammatory gene regulation by GR, highlighting GR's regulatory specificity conferred by context-dependent changes in chromatin architecture and transcription factor or co-regulator dynamics. GR controls both gene activation and repression, with the repression mechanism being central to favourable clinical outcomes. We describe current knowledge about 3D genome organisation and its role in spatiotemporal transcriptional control by GR. Looking beyond, we summarise the evidence for dynamics in gene regulation by GR through cooperative convergence of epigenetic modifications, transcription factor crosstalk, molecular condensate formation and chromatin looping. Further characterising these genomic events will reframe our understanding of mechanisms of transcriptional repression by GR.
AU  - Strickland, B.A.*
AU  - Ansari, S.A.
AU  - Dantoft, W.
AU  - Uhlenhaut, N.H.
C1  - 65484
C2  - 52203
SP  - 2596-2616
TI  - How to tame your genes: Mechanisms of inflammatory gene repression by glucocorticoids.
JO  - FEBS Lett.
VL  - 596
IS  - 20
PY  - 2022
SN  - 0014-5793
ER  - 

TY  - JOUR
AB  - Mitochondrial disorders are monogenic disorders characterized by a defect in oxidative phosphorylation and caused by pathogenic variants in one of over 340 different genes. The implementation of whole exome sequencing has led to a revolution in their diagnosis, duplicated the number of associated disease genes, and significantly increased the diagnosed fraction. However, the genetic etiology of a substantial fraction of patients exhibiting mitochondrial disorders remains unknown, highlighting limitations in variant detection and interpretation, which calls for improved computational and DNA sequencing methods, as well as the addition of OMICS tools. More intriguingly, this also suggests that some pathogenic variants lie outside of the protein-coding genes and that the mechanisms beyond the Mendelian inheritance and the mtDNA are of relevance. This review covers the current status of the genetic basis of mitochondrial diseases, discusses current challenges and perspectives, and explores the contribution of factors beyond the protein-coding regions and monogenic inheritance in the expansion of the genetic spectrum of disease.
AU  - Gusic, M.
AU  - Prokisch, H.
C1  - 61455
C2  - 50261
CY  - 111 River St, Hoboken 07030-5774, Nj Usa
SP  - 1132-1158
TI  - Genetic basis of mitochondrial diseases.
JO  - FEBS Lett.
VL  - 595
IS  - 8
PB  - Wiley
PY  - 2021
SN  - 0014-5793
ER  - 

TY  - JOUR
AB  - The interaction between the Shiga toxin B-subunit (STxB) and its globotriaosylceramide receptor (Gb3) has a high potential for being exploited for targeted cancer therapy. The primary goal of this study was to evaluate the capacity of STxB to carry small molecules and proteins as cargo into cells. For this purpose, an assay was designed to provide real-time information about the StxB-Gb3 interaction as well as the dynamics and mechanism of the internalization process. The assay revealed the ability to distinguish the process of binding to the cell surface from internalization and presented the importance of receptor and STxB clustering for internalization. The overall setup demonstrated that the binding mechanism is complex, and the concept of affinity is difficult to apply. Hence, time-resolved methods, providing detailed information about the interaction of STxB with cells, are critical for the optimization of intracellular delivery.
AU  - Encarnação, J.C.*
AU  - Napolitano, V.*
AU  - Opassi, G.*
AU  - Danielson, U.H.*
AU  - Dubin, G.*
AU  - Popowicz, G.M.
AU  - Munier-Lehmann, H.*
AU  - Buijs, J.*
AU  - Andersson, K.*
AU  - Björkelund, H.*
C1  - 59243
C2  - 48679
CY  - 111 River St, Hoboken 07030-5774, Nj Usa
SP  - 2406-2420
TI  - A real-time cell-binding assay reveals dynamic features of STxB-Gb3 cointernalization and STxB-mediated cargo delivery into cancer cells.
JO  - FEBS Lett.
VL  - 594
IS  - 15
PB  - Wiley
PY  - 2020
SN  - 0014-5793
ER  - 

TY  - JOUR
AB  - Coxsackievirus B3 (CVB3) has potential as a new oncolytic agent for the treatment of cancer but can induce severe pancreatitis. Here, we inserted target sequences of the microRNA miR-375 (miR-375TS) into the 5′ terminus of the polyprotein encoding sequence or into the 3′UTR of the CVB3 strain rCVB3.1 to prevent viral replication in the pancreas. In pancreatic EndoC-βH1 cells expressing miR-375 endogenously, replication of the 5′-miR-375TS virus and that of the 3′-miR-375TS virus was reduced by 4 × 103-fold and 3.9 × 104-fold, respectively, compared to the parental rCVB3.1. In colorectal carcinoma cells, replication and cytotoxicity of both viruses were slightly reduced compared to rCVB3.1, but less pronounced for the 3′-miR-375TS virus. Thus, CVB3 with miR-375TS in the 3′UTR of the viral genome may be suitable to avoid pancreatic toxicity.
AU  - Pryshliak, M.*
AU  - Hazini, A.*
AU  - Knoch, K.-P.
AU  - Dieringer, B.*
AU  - Tolksdorf, B.*
AU  - Solimena, M.
AU  - Kurreck, J.*
AU  - Pinkert, S.*
AU  - Fechner, H.*
C1  - 57376
C2  - 47742
CY  - 111 River St, Hoboken 07030-5774, Nj Usa
SP  - 763-775
TI  - MiR-375-mediated suppression of engineered coxsackievirus B3 in pancreatic cells.
JO  - FEBS Lett.
VL  - 594
IS  - 4
PB  - Wiley
PY  - 2019
SN  - 0014-5793
ER  - 

TY  - JOUR
AB  - Ras homolog enriched in brain (Rheb) is a small GTPase that regulates mammalian/mechanistic target of rapamycin complex 1 (mTORC1) and, thereby, cell growth and metabolism. Here we show that cycling between the inactive GDP-and the active GTP-bound state modulates the backbone dynamics of a C-terminal truncated form, RhebDCT, which is suggested to influence its interactions. We further investigated the interactions between RhebDCT and the proposed Rheb-binding domain of the regulatory protein FKBP38. The observed weak interactions with the GTP-analogue(GppNHp-) but not the GDP-bound state, appear to accelerate the GDP to GTP exchange, but only very weakly compared to a genuine GEF. Thus, FKBP38 is most likely not a GEF but a Rheb effector that may function in membrane targeting of Rheb.
AU  - de Cicco, M.*
AU  - Kiss, L.*
AU  - Dames, S.A.
C1  - 52579
C2  - 44059
CY  - Hoboken
SP  - 130-146
TI  - NMR analysis of the backbone dynamics of the small GTPase Rheb and its interaction with the regulatory protein FKBP38.
JO  - FEBS Lett.
VL  - 592
IS  - 1
PB  - Wiley
PY  - 2017
SN  - 0014-5793
ER  - 

TY  - JOUR
AB  - The conserved C-terminal FATC domain of the kinase 'target of rapamycin' is important for its regulation and was suggested to contain a peripheral membrane anchor. Here, we present the characterization of the interactions of the yeast TOR1 FATC domain (2438-2470 = y1fatc) and 15 mutants with membrane mimetic micelles, bicelles, and small unilamellar vesicles (SUVs) by NMR and CD spectroscopy. Replacement of up to 6-7 residues did not result in a significant abrogation of the association with micelles or bicelles. However, replacement of only one residue could result in an impairment of the interaction with SUVs that are usually used at low concentrations. Some mutants not binding liposomes may be introduced in full-length TOR for future functional and localization studies in vivo.
AU  - Sommer, L.A.*
AU  - Dames, S.A.
C1  - 31012
C2  - 34112
CY  - Amsterdam
SP  - 1755-1766
TI  - Characterization of residue-dependent differences in the peripheral membrane association of the FATC domain of the kinase 'target of rapamycin' by NMR and CD spectroscopy.
JO  - FEBS Lett.
VL  - 588
IS  - 9
PB  - Elsevier Science Bv
PY  - 2014
SN  - 0014-5793
ER  - 

TY  - JOUR
AB  - We generated transgenic mice to study the in vivo role of the cytoplasmic domain of human proEGF (proEGFcyt). Post-pubertal proEGFcyt transgenic (tg) mice displayed an up to 15% reduction in body weight, including smaller kidney and brain weights as compared to control littermates. Renal histology, gene expression profiles, and functional parameters were normal. In both sexes, serum levels of IGFBP-3 were reduced. Circulating IGF-I/IGF-II levels were unchanged. Histomorphological analysis revealed isolated foci of liver necrosis specific to proEGFcyt tg mice. In conclusion, we identified proEGF cytoplasmic domain as a novel modulator of whole body and organ-specific growth in mice.
AU  - Klonisch, T.*
AU  - Glogowska, A.*
AU  - Gratao, A.A.*
AU  - Grzech, M.*
AU  - Nistor, A.*
AU  - Torchia, M.*
AU  - Weber, E.*
AU  - Hrabě de Angelis, M.
AU  - Rathkolb, B.
AU  - Hoang-Vu, C.*
AU  - Wolf, E.*
AU  - Schneider, M.R.*
C1  - 1688
C2  - 26156
SP  - 1349-1357
TI  - The C-terminal cytoplasmic domain of human proEGF is a negative modulator of body and organ weights in transgenic mice.
JO  - FEBS Lett.
VL  - 583
IS  - 8
PB  - Elsevier Science Bv
PY  - 2009
SN  - 0014-5793
ER  - 

TY  - JOUR
AB  - The transport protein particle (TRAPP) complex is required for proper vesicular transport from the ER to the Golgi. The composition of yeast TRAPP is well characterized, but the organization of mammalian TRAPP complex remains elusive. Using a tandem affinity purification (TAP) approach, we provide first experimental proof for the association of NIBP (NIK/IKKbeta binding protein) with Bet3 and find two human paralogs of Trs33 (A and B) associated with Bet3. Interaction studies and gel filtration analysis reveal that both proteins are part of human TRAPP and might mark two distinct isocomplexes that exert different functions in the regulation of ER-to-Golgi traffic.
AU  - Kümmel, D.*
AU  - Oeckinghaus, A.
AU  - Wang, C.*
AU  - Krappmann, D.
AU  - Heinemann, U.*
C1  - 2929
C2  - 25806
SP  - 3729-3733
TI  - Distinct isocomplexes of the TRAPP trafficking factor coexist inside human cells.
JO  - FEBS Lett.
VL  - 582
IS  - 27
PB  - Elsevier
PY  - 2008
SN  - 0014-5793
ER  - 

TY  - JOUR
AB  - Feeding insects introduce oral secretions (OS) into the wounded tissue of the attacked plant. Various OS-derived molecules must be involved in subsequent processes including the induction of plant defence reactions. Using the planar lipid bilayer membrane technique, isolated OS were analyzed with respect to their membrane activities. Transmembrane ion fluxes were generated by OS of eight different lepidopteran larvae, all of which form comparable ion channels in artificial membranes. Currents were characterized by long lasting open times and conductivities from 250pS up to 1100pS. Channels formed by Spodoptera exigua secretions showed a preference for cations over anions. OS also induced a transient increase of the cytosolic calcium concentration in soybean cells, determined by the aequorin technique. Known compounds of the OS, fatty-acid-glutamine conjugates, also interfered with the membrane but were unable to form stable channels. Since ion fluxes and depolarization are early responses upon insect feeding, OS-derived components may be involved in the elicitation process by direct interaction with the plant membranes.
AU  - Maischak, H.
AU  - Grigoriev, P.A.*
AU  - Vogel, H.*
AU  - Boland, W.*
AU  - Mithöfer, A.*
C1  - 1729
C2  - 24902
SP  - 898-904
TI  - Oral secretions from herbivorous lepidopteran larvae exhibit ion channel-forming activities.
JO  - FEBS Lett.
VL  - 581
IS  - 5
PB  - Elsevier
PY  - 2007
SN  - 0014-5793
ER  - 

TY  - JOUR
AU  - Antonov, A.V.
AU  - Mewes, H.-W.
C1  - 4915
C2  - 24031
SP  - 844-848
TI  - BIOREL: The benchmark resource to estimate the relevance of the gene networks.
JO  - FEBS Lett.
VL  - 580
PY  - 2006
SN  - 0014-5793
ER  - 

TY  - JOUR
AU  - Sandermann, H.
C1  - 22232
C2  - 20968
SP  - 340-342
TI  - High free energy of lipid/protein interaction in biological membranes.
JO  - FEBS Lett.
VL  - 514
PY  - 2002
SN  - 0014-5793
ER  - 

TY  - JOUR
AB  - The role of thiols as oxidant scavengers during inactivation of bovine glucose-6-phosphate dehydrogenase by metal-catalyzed oxidation systems has been studied in vitro. Partial inactivation of the enzyme was achieved by the metal-catalyzed oxidation systems Fe(II)/H202/EDTA or Fe(II)/H202/ADP under specific conditions. When EDTA as chelator was present in the oxidation system, both cysteine and N-acetylcysteine at low concentrations (0.1-1 mM) drastically enhanced inactivation, while cysteinyl-glycine and glutathione did not. The thiol-mediated inactivation was inhibitable by superoxide dismutase. Depletion of enzyme activity by cysteine was paralleled by an increase of the carbonyl content, which indicates oxidative injury. However, when EDTA as chelator was replaced by the natural chelator ADP, all thiols studied acted as antioxidants. It is therefore concluded that the nature of the chelator as a constituent of the metal-catalyzed oxidation systems determines whether the antioxidative function of some thiols is shifted to a prooxidative function against glucose-6-phosphate dehydrogenase.
AU  - Maier, K.L.
AU  - Hinze, H.
AU  - Meyer, B.
AU  - Lenz, A.-G.
C1  - 28747
C2  - 33542
SP  - 95-98
TI  - Metal-catalyzed inactivation of bovine glucose-6-phosphate dehydrogenase - role of thiols.
JO  - FEBS Lett.
VL  - 396
IS  - 1
PY  - 1996
SN  - 0014-5793
ER  - 

TY  - JOUR
AB  - For plants, glutathione conjugation is a major pathway to detoxify organic xenobiotic. Glutathione S-conjugates (SG-conjugates) are formed in the cytosol, the in vitro transport over the tonoplast has been described and a final storage in the vacuole has been postulated. We show here that alachlor rapidly accumulates as GS-conjugates in the plant vacuole and that the first step of its degradation, the formation of the respective gamma-glutamylcysteinyl-S-conjugate, is catalyzed by a vacuolar carboxypeptidase, These results suggest the glutathione conjugate as a transport form but not a storage form of xenobiotic molecules.
AU  - Wolf, A.E.
AU  - Dietz, K.-J.
AU  - Schröder, P.
C1  - 23295
C2  - 31191
SP  - 31-34
TI  - Degradation of glutathione S-conjugates by a carboxypeptidase in the plant vacuole.
JO  - FEBS Lett.
VL  - 384
IS  - 1
PB  - Elsevier
PY  - 1996
SN  - 0014-5793
ER  - 

TY  - JOUR
AB  - In Chang liver cells we studied the influence of polychlorinated biphenyls (PCB) on the expression of different protooncogenes. In cells incubated with medium supplemented with PCB we observed an early effect after 3 h on c-erbA and c-erbH RNA level. This reduction of RNA was due to a delayed transcriptional activation of the genes. The PCB congener 3,3',4,4',5-pentachlorobiphenyl (5-CB) in a 1,000-fold lower concentration influenced protooncogene expression in the same manner. In contrast c-raf RNA level increased transiently after 24 h. The fact that persistent chemicals like PCB interfere with protooncogene expression is particularly interesting in view of their tumor promoting activity.
AU  - Hornhardt, S.V.
AU  - Jenke, H.S.
AU  - Michel, G.
C1  - 33539
C2  - 40089
SP  - 185-188
TI  - Polychlorinated biphenyls modulate protooncogene expression in Chang liver cells.
JO  - FEBS Lett.
VL  - 339
IS  - 1-2
PY  - 1994
SN  - 0014-5793
ER  - 

TY  - JOUR
AU  - Mischak, H.
AU  - Goodnight, J.
AU  - Henderson, D.W.
AU  - Osada, S.
AU  - Ohno, S.
AU  - Mushinski, J.F.
C1  - 20611
C2  - 13822
SP  - 51-55
TI  - Unique Expression Pattern of Protein Kinase C-0: High mRNA Levels in Normal Mouse Testes and in T-lymphocytic Cells and Neoplasms.
JO  - FEBS Lett.
VL  - 326
PY  - 1993
SN  - 0014-5793
ER  - 

TY  - JOUR
AB  - The effects of several amphipathic peptides on HIV-1 production in persistently infected cells are described. Melittin, a 26 amino acid α-helical amphipathic peptide, reduces HIV-1 production dose-dependently, whereas other amphipathic peptides do not. Six melittin derivatives which retain the α-helical portion have similar effects as melittin. The reduction of viral infectivity is not due to an effect of melittin on the virus particles but to an intracellular action of the peptide, which is readily taken up into cells, as shown by quantitative ELISA. Western blots of cells from melittin-treated cultures suggest that the processing of the gag/pol precursor is impaired.
AU  - Wachinger, M.
AU  - Særmark, T.
AU  - Erfle, V.F.
C1  - 40573
C2  - 38775
SP  - 235-241
TI  - Influence of amphipathic peptides on the HIV-1 production in persistently infected T lymphoma cells.
JO  - FEBS Lett.
VL  - 309
IS  - 3
PY  - 1992
SN  - 0014-5793
ER  - 

TY  - JOUR
AB  - Murine bone marrow-derived mast cells proliferate in response to interleukin 3. In addition to 6-biopterin, 7-biopterin was identified in these cells by HPLC analysis of iodine oxidized extracts and by alkaline permanganate oxidation to the 6- and 7-carboxylic acids. 7-Biopterin comprised 31.9 (±7.7)% of the total biopterin. It was absent in cells which were grown with of L-p-chlorophenylalanine, an inhibitor of tryptophan 5-monooxygenase. Both 6- and 7-biopterin were present in the cell as their tetrahydro forms. From these data we conclude that 7-biopterin, in contrast to e.g. brain tissue, regularly occurs as a normal metabolite in primary mast cells and that it is generated during hydroxylation of tryptophan.
AU  - Ziegler, I.
AU  - Hültner, L.
C1  - 40631
C2  - 13097
SP  - 147-150
TI  - Tetrahydro-6-biopterin is associated with tetrahydro-7-biopterin in primary murine mast cells.
JO  - FEBS Lett.
VL  - 307
IS  - 2
PY  - 1992
SN  - 0014-5793
ER  - 

TY  - JOUR
AU  - Fink, E.
AU  - Hehlein-Fink, C.
AU  - Eulitz, M.
C1  - 18781
C2  - 11896
SP  - 222-224
TI  - Amino Acid Sequence Elucidation of Human Acrosin-Trypsin Inhibitor (HUSI-II) Reveals that Kazal-Type Proteinase Inhibitors are Structurally Related to ß-subunits of Glycoprotein Hormones.
JO  - FEBS Lett.
VL  - 270
PY  - 1990
SN  - 0014-5793
ER  - 

TY  - JOUR
AB  - The triplex structure in vitro is well established; however, no direct evidence has been available concerning its existence in the cell. Using the direct chemical probing here we show that the triplex H structure can exist in E. coli cells at acidic intracellular pH values; this structure differs in some details from that observed in vitro.
AU  - Karlovsky, P.
AU  - Pečinka, P.*
AU  - Vojtíšková, M.*
AU  - Makaturova, E.*
AU  - Paleček, E.*
C1  - 34145
C2  - 36395
SP  - 39-42
TI  - Protonated triplex DNA in E. coli cells as detected by chemical probing.
JO  - FEBS Lett.
VL  - 274
IS  - 1-2
PY  - 1990
SN  - 0014-5793
ER  - 

TY  - JOUR
AB  - Cells obtained from bronchoalveolar lavage, or neutrophils of peripheral blood of dog, were incubated with the canine surfactant-associated protein A (SP-A). A significant decrease of the production of Superoxide anion was observed after subsequent stimulation with phorbol-12-myristate-13-acetate (PMA) as measured by the lucigenin-dependent chemiluminesence (CL). Several other proteins used for control experiments did not decrease lucigenin-dependent CL, indicating a specific effect of SP-A on phagocytes. Treatment of SP-A with collagenase prior to incubation with neutrophils destroyed the depleting effect on oxygen radical production of PMA-stimulated cells. We propose that SP-A acts as a regulatory factor of the respitatory burst of alveolar macrophages and neutrophils in the lungs. The inhibitory effect of SP-A is down-regulated by collagenase released from stimulated alveolar macrophages.
AU  - Weber, H.
AU  - Heilmann, P.
AU  - Meyer, B.
AU  - Maier, K.L.
C1  - 18277
C2  - 11498
SP  - 90-94
TI  - Effect of canine surfactant protein (SP-A) on the respiratory burst of phagocytic cells.
JO  - FEBS Lett.
VL  - 270
IS  - 1-2
PY  - 1990
SN  - 0014-5793
ER  - 

TY  - JOUR
AB  - Lectin stimulation of human T lymphocytes causes a 7-fold increase in the specific activities of GTP-cyclohydrolase and a 4-fold increase in the specific activities of sepiapterin reductase. GTP-cyclohydrolase activities are maximal after 48 h and subsequently decline, whereas sepiapterin reductase activities contunue to increase during the 72 h period measured. The specific activities of 6-pyruvoyltetrahydropterin synthase remain unchanged upon stimulation. Tetrahydrobiopterin synthesis during blast transformation is thus directed by both GTP-cyclohydrolase and sepiapterin reductase.
AU  - Kerler, F.
AU  - Ziegler, I.
AU  - Schwarzkopf, B.
AU  - Bacher, A.
C1  - 42603
C2  - 36442
SP  - 622-624
TI  - Regulation of tetrahydrobiopterin synthesis during lectin stimulation of human peripheral blood lymphocytes.
JO  - FEBS Lett.
VL  - 250
IS  - 2
PY  - 1989
SN  - 0014-5793
ER  - 

TY  - JOUR
AB  - Oxidation of the reactive site methionine (Met) in alpha-1-proteinase inhibitor (alpha-1-PI) to methionine sulfoxide (Met(O] is known to cause depletion of its elastase inhibitory activity. To estimate the selectivity of different oxidants in converting Met to Met(O) in alpha-1-PI, we measured the molar ratio Met(O)/alpha-1-PI at total inactivation. This ratio was determined to be 1.2 for both the myeloperoxidase/H2O2/chloride system and the related compound NH2Cl. With taurine monochloramine, another myeloperoxidase-related oxidant, 1.05 mol Met(O) were generated per mol alpha-1-PI during inactivation. These oxidants attack preferentially one Met residue in alpha-1-PI, which is identical with Met 358, as concluded from the parallelism of loss of elastase inhibitory activity and oxidation of Met. A similar high specificity for Met oxidation was determined for the xanthine oxidase-derived oxidants. In contrast, the ratio found for ozone and m-chloroperoxybenzoic acid was 6.0 and 5.0, respectively, indicating oxidation of additional Met residues besides the relative site Met in alpha-1-PI, i.e. unselective action of these oxidants. Further studies were performed on the efficiency of oxidants for total depletion of the elastase inhibitory capacity of alpha-1-PI. Ozone and m-chloroperoxybenzoic acid were 10-fold less effective and the superoxide anion/hydroxyl radicals were 30-50-fold less effective to inactivate the elastase inhibitory activity as compared to the myeloperoxidase-derived oxidants. The myeloperoxidase-related oxidants are discussed as important regulators of alpha-1-PI activity in vivo.
AU  - Maier, K.L.
AU  - Matejkova, E.
AU  - Hinze, H.
AU  - Leuschel, L.
AU  - Weber, H.
AU  - Beck-Speier, I.
C1  - 17593
C2  - 10868
SP  - 221-226
TI  - Different Selectivities of Oxidants During Oxidation of Methionine Residues in the (alpha)-1- proteinase Inhibitor.
JO  - FEBS Lett.
VL  - 250
IS  - 2
PY  - 1989
SN  - 0014-5793
ER  - 

TY  - JOUR
AB  - Oxidation of the reactive site methionine (Met) in α-1-proteinase inhibitor (α-1-PI) to methionine sulfoxide (Met(O)) is known to cause depletion of its elastase inhibitory activity. To estimate the selectivity of different oxidants in converting Met to Met(O) in α-1-PI, we measured the molar ratio Met(O)/α-1-PI at total inactivation. This ratio was determined to be 1.2 for both the myeloperoxidase/H2O2/chloride system and the related compound NH2Cl. With taurine monochloramine, another myeloperoxidase-related oxidant, 1.05 mol Met(O) were generated per mol α-1-PI during inactivation. These oxidants attack preferentially one Met residue in α-1-PI, which is identical with Met 358, as concluded from the parallelism of loss of elastase inhibitory activity and oxidation of Met. A similar high specificity for Met oxidation was determined for the xanthine oxidase-derived oxidants. In contrast, the ratio found for ozone and m-chloroperoxybenzoic acid was 6.0 and 5.0, respectively, indicating oxidation of additional Met residues besides the reactive site Met in α-1-PI, i.e. unselective action of these oxidants. Further studies were performed on the efficiency of oxidants for total depletion of the elastase inhibitory capacity of α-1-PI. Ozone and m-chloroperoxybenzoic acid were 10-fold less effective and the superoxide anion/hydroxyl radicals were 30-50-fold less effective to inactivate the elastase inhibitory activity as compared to the myeloperoxidase-derived oxidants. The myeloperoxidase-related oxidants are discussed as important regulators of α-1-PI activity in vivo.
AU  - Maier, K.L.
AU  - Matejkova, E.
AU  - Hinze, H.
AU  - Leuschel, L.
AU  - Weber, H.P.
AU  - Beck-Speier, I.
C1  - 42359
C2  - 36433
SP  - 221-226
TI  - Different selectivities of oxidants during oxidation of methionine residues in the α-1-proteinase inhibitor.
JO  - FEBS Lett.
VL  - 250
IS  - 2
PY  - 1989
SN  - 0014-5793
ER  - 

TY  - JOUR
AU  - Reddington, M.
AU  - Klotz, K.-N.
AU  - Lohse, M.J.
AU  - Hietel, B.
C1  - 19290
C2  - 11255
SP  - 125-128
TI  - Radiation Inactivation Analysis sof the A1 Adenosine Receptor of Rat Brain. Decrease in Radiation Inactivation Size in the Presence of Guanine Nucleotide.
JO  - FEBS Lett.
VL  - 252
PY  - 1989
SN  - 0014-5793
ER  - 

TY  - JOUR
AU  - Lenz, A.-G.
AU  - Holzer, H.
C1  - 40939
C2  - 38290
SP  - 271-274
TI  - Rapid reversible inactivation of fructose-1,6-bisphosphatase in Saccharomyces cerevisiae by glucose.
JO  - FEBS Lett.
VL  - 109
IS  - 2
PY  - 1980
SN  - 0014-5793
ER  - 

TY  - JOUR
AU  - Heldt, H.W.*
AU  - Chon, C.*
AU  - Lorimer, G.H.
C1  - 41777
C2  - 38178
SP  - 234-240
TI  - Phosphate requirement for the light activation of ribulose- 1,5-biphosphate carboxylase in intact spinach chloroplasts.
JO  - FEBS Lett.
VL  - 92
IS  - 2
PY  - 1978
SN  - 0014-5793
ER  -