TY  - JOUR
AB  - Septins are highly conserved GTP-binding proteins involved in numerous cellular processes. Despite a growing awareness of their roles in the cell biology, development and signal transmission in nervous systems, comparably little is known about precise septin expression. Here, we use the well-established model organism zebrafish (Danio rerio) to unravel the expression of sept8a and sept8b, with special focus on the CNS. We performed whole mount RNA in situ hybridization on zebrafish 1-4 dpf in combination with serial sectioning of epon-embedded samples as well as on brain sections of adult zebrafish to obtain precise histological mapping of gene expression. Our results show a common expression of both genes at embryonic stages, whereas sept8a is mainly restricted to the gill arches and sept8b to specific brain structures at later stages. Brains of adult zebrafish reveal a large spatial overlap of sept8a and sept8b expression with few regions uniquely expressing sept8a or sept8b. Our results indicate a neuronal expression of both genes, and additionally suggest expression of sept8b in glial cells. Altogether, this study provides a first detailed insight into the expression of sept8a and sept8b in zebrafish and contributes to a more comprehensive understanding of septin biology in vertebrate model systems.
AU  - Berger, C.*
AU  - Helmprobst, F.*
AU  - Chapouton, P.
AU  - Lillesaar, C.*
AU  - Stigloher, C.*
C1  - 50941
C2  - 42645
CY  - Amsterdam
SP  - 8-21
TI  - sept8a and sept8b mRNA expression in the developing and adult zebrafish.
JO  - Gene Expr. Patterns
VL  - 25-26
PB  - Elsevier Science Bv
PY  - 2017
SN  - 1567-133X
ER  - 

TY  - JOUR
AB  - MicroRNAs (miRNAs) have regulatory functions during vertebrate embryogenesis. They are short approximately 21bp long endogenously expressed single-stranded RNAs, which preferentially bind to complementary sequences in the 3' untranslated regions (UTR) of mRNAs and typically down-regulate the respective target mRNAs by translational repression or enhanced mRNA degradation. The Notch ligand Delta-like 1 (Dll1) is expressed in a highly dynamic pattern and has pleiotropic functions during embryogenesis and in adult tissues. Here, we report an interspecies in silico analysis to identify 16 miRNAs, which potentially bind to the mouse, human and chicken Dll1 3'UTRs. To analyze whether these miRNAs could regulate Dll1 gene expression during somitogenesis and neurogenesis, we performed a systematic whole mount in situ hybridisation screen, followed by radioactive in situ hybridisation on sections, using LNA modified DNA probes in mouse embryos. We find that 7 miRNAs (miR-34a, miR-103, miR-107, miR-130a, miR-130b, miR-449a and miR-449c) are expressed in developing somites, limbs, restricted regions of the brain and neural tube between 9.5 dpc and 12.5 dpc. This suggests that these miRNAs could possibly target the Dll1 3'UTR in these regions. The other miRNAs are not expressed or below the detection limit and thus are unlikely to regulate Dll1 at the analyzed embryonic stages.
AU  - Hösel, B.G.
AU  - Bhujabal, Z.
AU  - Przemeck, G.K.H.
AU  - Kurz-Drexler, A.
AU  - Vogt Weisenhorn, D.M.
AU  - Hrabě de Angelis, M.
AU  - Beckers, J.
C1  - 563
C2  - 27350
SP  - 265-273
TI  - Combination of in silico and in situ hybridisation approaches to identify potential Dll1 associated miRNAs during mouse embryogenesis.
JO  - Gene Expr. Patterns
VL  - 10
IS  - 6
PB  - Elsevier
PY  - 2010
SN  - 1567-133X
ER  - 

TY  - JOUR
AU  - Schneider, M.
AU  - Vogt Weisenhorn, D.M.
AU  - Seiler, A.
AU  - Bornkamm, G.W.
AU  - Brielmeier, M.
AU  - Conrad, M.
C1  - 5697
C2  - 23833
SP  - 489-494
TI  - Embryonic expression profile of phospholipid hydroperoxide glutathione peroxidase.
JO  - Gene Expr. Patterns
VL  - 6
PY  - 2006
SN  - 1567-133X
ER  - 

TY  - JOUR
AU  - Loeb-Hennard, C.
AU  - Kremmer, E.
AU  - Bally-Cuif, L.
C1  - 5100
C2  - 22308
SP  - 341-347
TI  - Prominent transcription of zebrafish N-myc (nmyc1) in tectal and retinal growth zones during embryonic and early larval development.
JO  - Gene Expr. Patterns
VL  - 5
PY  - 2005
SN  - 1567-133X
ER  - 

TY  - JOUR
AU  - Machka, C.
AU  - Kersten, M.*
AU  - Zobawa, M.*
AU  - Harder, A.*
AU  - Horsch, M.
AU  - Halder, T.*
AU  - Lottspeich, F.*
AU  - Hrabě de Angelis, M.
AU  - Beckers, J.
C1  - 2489
C2  - 23520
SP  - 94-101
TI  - Identification of Dll1 (Delta1) target genes during mouse embryogenesis using differential expression profiling.
JO  - Gene Expr. Patterns
VL  - 6
PY  - 2005
SN  - 1567-133X
ER  - 

TY  - JOUR
AU  - Adolf, B.*
AU  - Bellipanni, G.*
AU  - Huber, V.*
AU  - Bally-Cuif, L.
C1  - 2315
C2  - 22311
SP  - 35-41
TI  - atoh1.2 and beta3.1 are two new bHLH-encoding genes expressed in selective precursor cells of the zebrafish anterior hindbrain.
JO  - Gene Expr. Patterns
VL  - 5
PY  - 2004
SN  - 1567-133X
ER  - 

TY  - JOUR
AU  - Loeb-Hennard, C.
AU  - Cousin, X.*
AU  - Prengel, I.
AU  - Kremmer, E.
C1  - 2134
C2  - 22127
SP  - 91-96
TI  - Cloning and expression pattern of vat-1 homolog gene in zebrafish.
JO  - Gene Expr. Patterns
VL  - 5
PY  - 2004
SN  - 1567-133X
ER  -