TY  - JOUR
AB  - Sewage sludge (biosolids) management represents a worldwide issue. Due to its valuable properties, approximately one half of the EU production is recovered in agriculture. Nevertheless, growing attention is given to potential negative effects deriving from the presence of harmful pollutants. It is recognized that a (even very detailed) chemical characterization is not able to predict ecotoxicity of a mixture. However, this can be directly measured by bioassays. Actually, the choice of the most suitable tests is still under debate. This paper presents a multilevel characterization protocol of sewage sludge and other organic residues, based on bioassays and chemical-physical-microbiological analyses. The detailed description of the experimental procedure includes all the involved steps: the criteria for selecting the organic matrices to be tested and compared; the sample pre-treatment required before the analyses execution; the chemical, physical and microbiological characterisation; the bioassays, grouped in three classes (baseline toxicity; specific mode of action; reactive mode of action); data processing. The novelty of this paper lies in the integrated use of advanced tools, and is based on three pillars: • the direct ecosafety assessment of the matrices to be reused. • the adoption of innovative bioassays and analytical procedures. • the original criteria for data normalization and processing.
AU  - Bertanza, G.*
AU  - Abbà, A.*
AU  - Alias, C.*
AU  - Amatucci, A.*
AU  - Binelli, A.*
AU  - Castiglioni, S.*
AU  - Fossati, M.*
AU  - Cruzeiro, C.
AU  - Torre, C.D.*
AU  - Domini, M.*
AU  - Feretti, D.*
AU  - Gilioli, G.*
AU  - Magni, S.*
AU  - Mazzoleni, G.*
AU  - Menghini, M.*
AU  - Pedrazzani, R.*
AU  - Schröder, P.
AU  - Simonetto, A.*
AU  - Steimberg, N.*
AU  - Ventura, V.*
AU  - Vezzoli, S.*
AU  - Zerbini, I.*
C1  - 69998
C2  - 55356
CY  - Radarweg 29, 1043 Nx Amsterdam, Netherlands
TI  - To spread or not to spread? Assessing the suitability of sewage sludge and other biogenic wastes for agriculture reuse.
JO  - MethodsX
VL  - 12
PB  - Elsevier
PY  - 2024
SN  - 2215-0161
ER  - 

TY  - JOUR
AB  - In this study we present a highly versatile and easily configurable system for measuring plant electrophysiological parameters and ionic flow rates, connected to a computer-controlled highly accurate positioning device. The modular software used allows easy customizable configurations for the measurement of electrophysiological parameters. Both the operational tests and the experiments already performed have been fully successful and rendered a low noise and highly stable signal. Assembly, programming and configuration examples are discussed. The system is a powerful technique that not only gives precise measuring of plant electrophysiological status, but also allows easy development of ad hoc configurations that are not constrained to plant studies. We developed a highly modular system for electrophysiology measurements that can be used either in organs or cells and performs either steady or dynamic intra- and extracellular measurements that takes advantage of the easiness of visual object-oriented programming High precision accuracy in data acquisition under electrical noisy environments that allows it to run even in a laboratory close to electrical equipment that produce electrical noise. The system makes an improvement of the currently used systems for monitoring and controlling high precision measurements and micromanipulation systems providing an open and customizable environment for multiple experimental needs.
AU  - Gunse, B.*
AU  - Poschenrieder, C.*
AU  - Rankl, S.
AU  - Schröder, P.
AU  - Rodrigo-Moreno, A.*
AU  - Barcelo, J.*
C1  - 48699
C2  - 41254
SP  - 436-451
TI  - A highly versatile and easily configurable system for plant electrophysiology.
JO  - MethodsX
VL  - 3
PY  - 2016
SN  - 2215-0161
ER  - 

TY  - JOUR
AB  - A sensitive method is introduced to detect selected pharmaceutical residues and polar pesticides with UHPLC-MS in water samples of different origin. Active or passive water sampling was combined with a laboratory solid-phase extraction cleanup and stable isotope dilution analysis. Recovery experiments demonstrated that the internal standard correction performed well for the compensation of matrix effects. Besides, the original targeted analysis approach was expanded by non-target analysis of the samples with only one more consecutive injection run needed. The key benefits of this multi-residue analysis are:Targeted analysis and quantification combined with non-target analysis on a micro-scale UHPLC-MS system usually employed for qualitative analysis only.The internal standards for targeted analysis were used in non-target runs to calculate the partition coefficient log P of unknown substances employing the retention time index (RTI).The filtering of database hits for two criteria (exact mass and partition coefficient) significantly reduced the list of suspects and at the same time rendered it possible to perform non-target analysis with lower mass accuracy (no lock-spray) in the range of 20-500 ppm.
AU  - Deyerling, D.
AU  - Schramm, K.-W.
C1  - 47343
C2  - 40532
SP  - 399-408
TI  - Integrated targeted and non-targeted analysis of water sample extracts with micro-scale UHPLC-MS.
JO  - MethodsX
VL  - 2
PY  - 2015
SN  - 2215-0161
ER  -