TY  - JOUR
AB  - An untargeted shot-gun approach is described for the ultra-high-resolution analysis of fennel proteins by Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) combined with a home-made Matlab search algorithm. The first step of the proposed bioinformatic strategy was the development of a custom-made fennel protein database, starting from the well-known, on-line available, protein NCBI database, under Foeniculum Vulgare organism, consisting of 231 total proteins. Partial and redundant forms of proteins, repeatedly included in the official NCBI database under different codes, were removed. In the final custom-made database, in addition to the 92 fennel specific non-redundant proteins, 10 proteins belonging to recognized allergenic sources associated with spice-mugwort-allergy syndrome (celery, carrot, parsley, birch, and mugwort) were also included. The second step was the in-silico enzymatic digestion, performed on all the 102 proteins, to obtain a theoretical list of m/z dataset of tryptic peptides. The Matlab processing data was the third and crucial step, necessary to search for in-silico mass calculated peptide sequences in the high resolution ICR mass spectra of the digested fennel extract. The final step was based on database searching in Peptide Mass Fingerprint (PMF) mode by using the matched m/z values as input data. The PMF search results confirmed the presence of 70 proteins (61 fennel specific and 9 allergenic proteins) inside the fennel extract.
AU  - Melfi, M.T.*
AU  - Kanawati, B.
AU  - Schmitt-Kopplin, P.
AU  - Macchia, L.*
AU  - Centonze, D.*
AU  - Nardiello, D.*
C1  - 61760
C2  - 50141
TI  - Data processing for fennel protein characterization by Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).
JO  - Data Brief
VL  - 35
PY  - 2021
ER  - 

TY  - JOUR
AB  - The data presented in this article describe the fatty acid composition of chow, liver tissue and isolated liver mitochondria from mice fed for 6-24 weeks with a high caloric western diet (WD) in comparison to control diet (normal diet, ND). The fatty acid composition was measured via gas chromatography flame ionization detection (GC-FID). Moreover, WD-induced mitochondrial protein changes are presented in this work and were analyzed by mass spectrometry (LC-MS/MS). For further interpretation and discussion of the presented data please refer to the research article entitled "Mitochondrial adaptation in steatotic mice" (Einer et al., 2017) [1].
AU  - Einer, C.
AU  - Hohenester, S.*
AU  - Wimmer, R.*
AU  - Wottke, L.*
AU  - Artmann, R.*
AU  - Schulz, S.
AU  - Gosmann, C.
AU  - Simmons, A.
AU  - Leitzinger, C.
AU  - Eberhagen, C.
AU  - Borchard, S.
AU  - Schmitt, S.*
AU  - Hauck, S.M.
AU  - von Toerne, C.
AU  - Jastroch, M.
AU  - Walheim, E.
AU  - Rust, C.*
AU  - Gerbes, A.L.*
AU  - Popper, B.*
AU  - Mayr, D.*
AU  - Schnurr, M.*
AU  - Vollmar, A.M.*
AU  - Denk, G.*
AU  - Zischka, H.
C1  - 52126
C2  - 44140
SP  - 163-169
TI  - Data on chow, liver tissue and mitochondrial fatty acid compositions as well as mitochondrial proteome changes after feeding mice a western diet for 6-24 weeks.
JO  - Data Brief
VL  - 15
PY  - 2017
ER  - 

TY  - JOUR
AB  - The data described in this article are related to “High levels of eukaryotic Initiation Factor 6 (eIF6) are required for immune system homeostasis and for steering the glycolytic flux of TCR-stimulated CD4 + T cells in both mice and humans” (Manfrini et al., in press) [1]. eIF6 is a translation initiation factor required for ribosomal biogenesis (Sanvito et al., 1999) [2] and for proper translational initiation (Gallo and Manfrini, 2015; Miluzio et al., 2016) [3,4] whose protein abundance requires tight regulation. Here we analyze by flow cytometry the effects of eIF6 depletion on proportions of specific innate and adaptive immune system subpopulations and on thymocyte maturation in mice.
AU  - Manfrini, N.*
AU  - Ricciardi, S.*
AU  - Miluzio, A.*
AU  - Fedeli, M.*
AU  - Scagliola, A.*
AU  - Gallo, S.*
AU  - Adler, T.
AU  - Busch, D.H.*
AU  - Gailus-Durner, V.
AU  - Fuchs, H.
AU  - Hrabě de Angelis, M.
AU  - Biffo, S.*
C1  - 51907
C2  - 43900
SP  - 653-658
TI  - Data on the effects of eIF6 downmodulation on the proportions of innate and adaptive immune system cell subpopulations and on thymocyte maturation.
JO  - Data Brief
VL  - 14
PY  - 2017
ER  - 

TY  - JOUR
AB  - Biokinetics data of lung-administered PEI F25-LMW/siRNA polyplexes within different lung compartments are presented. Thereby, at three different timepoints (1 h, 3 h, 8 h), the data was determined by calculations to the 32P-radioactivity in the whole mouse body. Additionally, data was optimized to the available PEI F25-LMW/siRNA polyplexes in the target organ and therefore normalized to the sum of all lung compartments. Methods, other biokinetics data and the discussion of the results are published in "Biokinetic studies of non-complexed siRNA versus nano-sized PEI F25-LMW/siRNA polyplexes following intratracheal instillation into mice" (Lipka et al., 2016 [1]).
AU  - Lipka, J.
AU  - Semmler-Behnke, M.
AU  - Wenk, A.
AU  - Burkhardt, J.*
AU  - Aigner, A.*
AU  - Kreyling, W.G.
C1  - 48458
C2  - 41155
SP  - 1175-1178
TI  - Biokinetic datasets of PEI F25-LMW complexed and non-complexed 32P-siRNA within different lung compartments.
JO  - Data Brief
VL  - 7
PY  - 2016
ER  - 

TY  - JOUR
AB  - © 2016 The Authors This article contains data related to the research article entitled “Altered relation of the renin-aldosterone system and vasoactive peptides in type 2 diabetes: the KORA F4 study” (Then et al., 2016) [1] and describes the association of the aldosterone to renin ratio with midregional-pro atrial natriuretic peptide (MR-proANP) and midregional-pro adrenomedullin (MR-proADM) in 1261 participants from the KORA F4 cohort.
AU  - Then, C.
AU  - Rottenkolber, M.*
AU  - Lechner, A.
AU  - Meisinger, C.
AU  - Heier, M.
AU  - Koenig, W.*
AU  - Peters, A.
AU  - Rathmann, W.*
AU  - Bidlingmaier, M.*
AU  - Reincke, M.*
AU  - Seissler, J.
C1  - 49382
C2  - 41815
SP  - 1395-1399
TI  - Dataset of the associations of aldosterone to renin ratio with MR-proANP and MR-proADM.
JO  - Data Brief
VL  - 8
PY  - 2016
ER  -