TY - JOUR AB - Soil-borne microorganisms can systemically affect shoot resistance to pathogens relying on jasmonic acid and/or salicylic acid. However, the emanating root triggers in these scenarios remain elusive. Here we identify an N-hydroxypipecolic-acid-(NHP-)directed, salicylic-acid-related mechanism of root-triggered systemic resistance in Arabidopsis, which uses components of systemic acquired resistance known in leaves. However, in contrast to the inductive nature of systemic acquired resistance, FLAVIN-DEPENDENT MONOOXYGENASE 1 (FMO1) continuously synthesizes NHP in roots, while the glucosyltransferase UGT76B1 concomitantly conjugates and immobilizes NHP. Physical grafting experiments and tissue-specific knockouts revealed that the loss of UGT76B1 in roots leads to enhanced NHP release, initiating shoot responses. This counteracting standby FMO1/UGT76B1 circuit is specifically and sensitively modulated by root-associated microorganisms. Endophytic and (hemi)biotrophic fungi induce UGT76B1 degradation and FMO1 expression, resulting in varying levels of NHP being released to the shoot, where this root signal differently modulates defence and growth. AU - Xu, P. AU - Fundneider, S. AU - Lange, B. AU - Maksym, R.P. AU - Stuttmann, J.* AU - Schäffner, A. C1 - 75222 C2 - 57861 CY - Heidelberger Platz 3, Berlin, 14197, Germany TI - A root-based N-hydroxypipecolic acid standby circuit to direct immunity and growth of Arabidopsis shoots. JO - Nat. Plants PB - Nature Portfolio PY - 2025 SN - 2055-026X ER - TY - JOUR AB - Centromeres strongly affect (epi)genomic architecture and meiotic recombination dynamics, influencing the overall distribution and frequency of crossovers. Here we show how recombination is regulated and distributed in the holocentric plant Rhynchospora breviuscula, a species with diffused centromeres. Combining immunocytochemistry, chromatin analysis and high-throughput single-pollen sequencing, we discovered that crossover frequency is distally biased, in sharp contrast to the diffused distribution of hundreds of centromeric units and (epi)genomic features. Remarkably, we found that crossovers were abolished inside centromeric units but not in their proximity, indicating the absence of a canonical centromere effect. We further propose that telomere-led synapsis of homologues is the feature that best explains the observed recombination landscape. Our results hint at the primary influence of mechanistic features of meiotic pairing and synapsis rather than (epi)genomic features and centromere organization in determining the distally biased crossover distribution in R. breviuscula, whereas centromeres and (epi)genetic properties only affect crossover positioning locally. AU - Castellani, M.* AU - Zhang, M.* AU - Thangavel, G.* AU - Mata-Sucre, Y.* AU - Lux, T. AU - Campoy, J.A.* AU - Marek, M.* AU - Huettel, B.* AU - Sun, H.* AU - Mayer, K.F.X. AU - Schneeberger, K.* AU - Marques, A.R.* C1 - 69942 C2 - 55328 CY - Heidelberger Platz 3, Berlin, 14197, Germany SP - 423-438 TI - Meiotic recombination dynamics in plants with repeat-based holocentromeres shed light on the primary drivers of crossover patterning. JO - Nat. Plants VL - 10 IS - 3 PB - Nature Portfolio PY - 2024 SN - 2055-026X ER - TY - JOUR AB - D6 PROTEIN KINASE (D6PK) is a polarly localized plasma-membrane-associated kinase from Arabidopsis thaliana that activates polarly distributed PIN-FORMED auxin transporters. D6PK moves rapidly to and from the plasma membrane, independent of its PIN-FORMED targets. The middle D6PK domain, an insertion between kinase subdomains VII and VIII, is required and sufficient for association and polarity of the D6PK plasma membrane. How D6PK polarity is established and maintained remains to be shown. Here we show that cysteines from repeated middle domain CXX(X)P motifs are S-acylated and required for D6PK membrane association. While D6PK S-acylation is not detectably regulated during intracellular transport, phosphorylation of adjacent serine residues, in part in dependence on the upstream 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE, promotes D6PK transport, controls D6PK residence time at the plasma membrane and prevents its lateral diffusion. We thus identify new mechanisms for the regulation of D6PK plasma membrane interaction and polarity. AU - Graf, A.* AU - Bassukas, A.E.L.* AU - Xiao, Y.* AU - Barbosa, I.C.R.* AU - Mergner, J.* AU - Grill, P. AU - Michalke, B. AU - Kuster, B.* AU - Schwechheimer, C.* C1 - 69854 C2 - 55285 CY - Heidelberger Platz 3, Berlin, 14197, Germany SP - 300-314 TI - D6PK plasma membrane polarity requires a repeated CXX(X)P motif and PDK1-dependent phosphorylation. JO - Nat. Plants VL - 10 IS - 2 PB - Nature Portfolio PY - 2024 SN - 2055-026X ER - TY - JOUR AU - Haberer, G. AU - Mayer, K.F.X. C1 - 65731 C2 - 52468 SP - 727-728 TI - Differential and complementary selection of heterotic groups. JO - Nat. Plants VL - 8 IS - 7 PY - 2022 SN - 2055-026X ER - TY - JOUR AB - In the version of this Article originally published, one affiliation of the author Pascal Falter-Braun was mistakenly omitted; they should have also been affiliated with the Department of Plant Systems Biology, Center of Life and Food Sciences Weihenstephan, Technische Universität München. This error has now been corrected. AU - Kulich, I.* AU - Vogler, F.* AU - Bleckmann, A.* AU - Cyprys, P.* AU - Lindemeier, M.* AU - Fuchs, I.* AU - Krassini, L.* AU - Schubert, T.* AU - Steinbrenner, J.* AU - Beynon, J.* AU - Falter-Braun, P. AU - Längst, G.* AU - Dresselhaus, T.* AU - Sprunck, S.* C1 - 60925 C2 - 50230 TI - Author Correction: ARMADILLO REPEAT ONLY proteins confine Rho GTPase signalling to polar growth sites (Nature Plants, (2020), 6, 10, (1275-1288), 10.1038/s41477-020-00781-1). JO - Nat. Plants VL - 7 PY - 2021 SN - 2055-026X ER - TY - JOUR AB - Upon first exposure to light, plants initiate the synchronized biogenesis of chlorophyll and thylakoid membranes. Two new studies have revealed a molecular view of the light-dependent step of chlorophyll synthesis within the membranes of developing angiosperm chloroplasts. AU - Wietrzynski, W. AU - Engel, B.D. C1 - 61830 C2 - 50467 CY - Heidelberger Platz 3, Berlin, 14197, Germany SP - 380-381 TI - Chlorophyll biogenesis sees the light. JO - Nat. Plants VL - 7 IS - 4 PB - Nature Research PY - 2021 SN - 2055-026X ER - TY - JOUR AB - Biogenesis of photosystem II (PSII), nature's water-splitting catalyst, is assisted by auxiliary proteins that form transient complexes with PSII components to facilitate stepwise assembly events. Using cryo-electron microscopy, we solved the structure of such a PSII assembly intermediate from Thermosynechococcus elongatus at 2.94 Å resolution. It contains three assembly factors (Psb27, Psb28 and Psb34) and provides detailed insights into their molecular function. Binding of Psb28 induces large conformational changes at the PSII acceptor side, which distort the binding pocket of the mobile quinone (QB) and replace the bicarbonate ligand of non-haem iron with glutamate, a structural motif found in reaction centres of non-oxygenic photosynthetic bacteria. These results reveal mechanisms that protect PSII from damage during biogenesis until water splitting is activated. Our structure further demonstrates how the PSII active site is prepared for the incorporation of the Mn4CaO5 cluster, which performs the unique water-splitting reaction. AU - Zabret, J.* AU - Bohn, S.* AU - Schuller, S.K.* AU - Arnolds, O.* AU - Möller, M.* AU - Meier-Credo, J.* AU - Liauw, P.* AU - Chan, A.* AU - Tajkhorshid, E.* AU - Langer, J.D.* AU - Stoll, R.* AU - Krieger-Liszkay, A.* AU - Engel, B.D. AU - Rudack, T.* AU - Schuller, J.M.* AU - Nowaczyk, M.M.* C1 - 61777 C2 - 50447 CY - Heidelberger Platz 3, Berlin, 14197, Germany SP - 524–538 TI - Structural insights into photosystem II assembly. JO - Nat. Plants VL - 7 PB - Nature Research PY - 2021 SN - 2055-026X ER - TY - JOUR AB - Approximately one-third of global CO2 fixation occurs in a phase-separated algal organelle called the pyrenoid. The existing data suggest that the pyrenoid forms by the phase separation of the CO2-fixing enzyme Rubisco with a linker protein; however, the molecular interactions underlying this phase separation remain unknown. Here we present the structural basis of the interactions between Rubisco and its intrinsically disordered linker protein Essential Pyrenoid Component 1 (EPYC1) in the model alga Chlamydomonas reinhardtii. We find that EPYC1 consists of five evenly spaced Rubisco-binding regions that share sequence similarity. Single-particle cryo-electron microscopy of these regions in complex with Rubisco indicates that each Rubisco holoenzyme has eight binding sites for EPYC1, one on each Rubisco small subunit. Interface mutations disrupt binding, phase separation and pyrenoid formation. Cryo-electron tomography supports a model in which EPYC1 and Rubisco form a codependent multivalent network of specific low-affinity bonds, giving the matrix liquid-like properties. Our results advance the structural and functional understanding of the phase separation underlying the pyrenoid, an organelle that plays a fundamental role in the global carbon cycle. AU - He, S.* AU - Chou, H.T.* AU - Matthies, D.* AU - Wunder, T.* AU - Meyer, M.T.* AU - Atkinson, N.* AU - Martinez-Sanchez, A.* AU - Jeffrey, P.D.* AU - Port, S.A.* AU - Patena, W.* AU - He, G.* AU - Chen, V.K.* AU - Hughson, F.M.* AU - McCormick, A.J.* AU - Mueller-Cajar, O.* AU - Engel, B.D. AU - Yu, Z.* AU - Jonikas, M.C.* C1 - 60594 C2 - 49403 CY - Heidelberger Platz 3, Berlin, 14197, Germany SP - 1480–1490 TI - The structural basis of Rubisco phase separation in the pyrenoid. JO - Nat. Plants VL - 6 PB - Nature Research PY - 2020 SN - 2055-026X ER - TY - JOUR AB - Polar growth requires the precise tuning of Rho GTPase signalling at distinct plasma membrane domains. The activity of Rho of plant (ROP) GTPases is regulated by the opposing action of guanine nucleotide-exchange factors (GEFs) and GTPase-activating proteins (GAPs). Whereas plant-specific ROPGEFs have been shown to be embedded in higher-level regulatory mechanisms involving membrane-bound receptor-like kinases, the regulation of GAPs has remained enigmatic. Here, we show that threeArabidopsisARMADILLO REPEAT ONLY (ARO) proteins are essential for the stabilization of growth sites in root hair cells and trichomes. AROs interact with ROP1 enhancer GAPs (RENGAPs) and bind to the plasma membrane via a conserved polybasic region at the ARO amino terminus. The ectopic spreading of ROP2 inaro2/3/4mutant root hair cells and the preferential interaction of AROs with active ROPs and anionic phospholipids suggests that AROs recruit RENGAPs into complexes with ROPs to confine ROP signalling to distinct membrane regions.Cell polarity that requires asymmetrical distribution of cellular components is important for plant growth and development. Here the authors identify a group of ARMADILLO domain proteins that collectively act in the polarized cell expansion of tip-growing cells by recruiting Rho of plant 1 (ROP1) enhancer GTPase-activating proteins and therefore ROP signalling to distinct plasma membrane sites. AU - Kulich, I.* AU - Vogler, F.* AU - Bleckmann, A.* AU - Cyprys, P.* AU - Lindemeier, M.* AU - Fuchs, I.* AU - Krassini, L.* AU - Schubert, T.* AU - Steinbrenner, J.* AU - Beynon, J.* AU - Falter-Braun, P. AU - Längst, G.* AU - Dresselhaus, T.* AU - Sprunck, S.* C1 - 60276 C2 - 49306 CY - Heidelberger Platz 3, Berlin, 14197, Germany SP - 1275–1288 TI - ARMADILLO REPEAT ONLY proteins confine Rho GTPase signalling to polar growth sites. JO - Nat. Plants VL - 6 IS - 10 PB - Nature Research PY - 2020 SN - 2055-026X ER - TY - JOUR AB - An amendment to this paper has been published and can be accessed via a link at the top of the paper. AU - Ricci, W.A.* AU - Lu, Z.* AU - Ji, L.* AU - Marand, A.P.* AU - Ethridge, C.L.* AU - Murphy, N.G.* AU - Noshay, J.M.* AU - Galli, M.* AU - Mejía-Guerra, M.K.* AU - Colomé-Tatché, M. AU - Johannes, F.* AU - Rowley, M.J.* AU - Corces, V.G.* AU - Zhai, J.* AU - Scanlon, M.J.* AU - Buckler, E.S.* AU - Gallavotti, A.* AU - Springer, N.M.* AU - Schmitz, R.J.* AU - Zhang, X.* C1 - 58648 C2 - 48199 TI - Author Correction: Widespread long-range cis-regulatory elements in the maize genome. JO - Nat. Plants VL - 6 IS - 3 PY - 2020 SN - 2055-026X ER - TY - JOUR AB - Genetic mapping studies on crops suggest that agronomic traits can be controlled by gene–distal intergenic loci. Despite the biological importance and the potential agronomic utility of these loci, they remain virtually uncharacterized in all crop species to date. Here, we provide genetic, epigenomic and functional molecular evidence to support the widespread existence of gene–distal (hereafter, distal) loci that act as long-range transcriptional cis-regulatory elements (CREs) in the maize genome. Such loci are enriched for euchromatic features that suggest their regulatory functions. Chromatin loops link together putative CREs with genes and recapitulate genetic interactions. Putative CREs also display elevated transcriptional enhancer activities, as measured by self-transcribing active regulatory region sequencing. These results provide functional support for the widespread existence of CREs that act over large genomic distances to control gene expression. AU - Ricci, W.A.* AU - Lu, Z.* AU - Ji, L.* AU - Marand, A.P.* AU - Ethridge, C.L.* AU - Murphy, N.G.* AU - Noshay, J.M.* AU - Galli, M.* AU - Mejía-Guerra, M.K.* AU - Colomé-Tatché, M. AU - Johannes, F.* AU - Rowley, M.J.* AU - Corces, V.G.* AU - Zhai, J.* AU - Scanlon, M.J.* AU - Buckler, E.S.* AU - Gallavotti, A.* AU - Springer, N.M.* AU - Schmitz, R.J.* AU - Zhang, X.* C1 - 57367 C2 - 47771 SP - 1237-1249 TI - Widespread long-range cis-regulatory elements in the maize genome. JO - Nat. Plants VL - 5 IS - 12 PY - 2019 SN - 2055-026X ER - TY - JOUR AB - Increasing the accuracy of crop productivity estimates is a key element in planning adaptation strategies to ensure global food security under climate change. Process-based crop models are effective means to project climate impact on crop yield, but have large uncertainty in yield simulations. Here, we show that variations in the mathematical functions currently used to simulate temperature responses of physiological processes in 29 wheat models account for >50% of uncertainty in simulated grain yields for mean growing season temperatures from 14 °C to 33 °C. We derived a set of new temperature response functions that when substituted in four wheat models reduced the error in grain yield simulations across seven global sites with different temperature regimes by 19% to 50% (42% average). We anticipate the improved temperature responses to be a key step to improve modelling of crops under rising temperature and climate change, leading to higher skill of crop yield projections. AU - Wang, E.* AU - Martre, P.* AU - Zhao, Z.* AU - Ewert, F.* AU - Maiorano, A.* AU - Rötter, R.P.* AU - Kimball, B.A.* AU - Ottman, M.J.* AU - Wall, G.W.* AU - White, J.W.* AU - Reynolds, M.P.* AU - Alderman, P.D.* AU - Aggarwal, P.K.* AU - Anothai, J.* AU - Basso, B.* AU - Biernath, C.J. AU - Cammarano, D.* AU - Challinor, A.J.* AU - de Sanctis, G.* AU - Doltra, J.* AU - Fereres, E.* AU - Garcia-Vila, M.* AU - Gayler, S.* AU - Hoogenboom, G.* AU - Hunt, L.A.* AU - Izaurralde, R.C.* AU - Jabloun, M.* AU - Jones, C.D.* AU - Kersebaum, K.C.* AU - Koehler, A.-K.* AU - Liu, L.* AU - Müller, C.* AU - Naresh Kumar, S.* AU - Nendel, C.* AU - O'Leary, G.* AU - Olesen, J.E.* AU - Palosuo, T.* AU - Priesack, E. AU - Eyshi Rezaei, E.* AU - Ripoche, D.* AU - Ruane, A.C.* AU - Semenov, M.A.* AU - Shcherbak, I.* AU - Stöckle, C.* AU - Stratonovitch, P.* AU - Streck,T.* AU - Supit, I.* AU - Tao, F.* AU - Thorburn, P.J.* AU - Waha, K.* AU - Wallach, D.* AU - Wang, Z.* AU - Wolf, J.* AU - Zhu, Y.* AU - Asseng, S.* C1 - 51546 C2 - 43194 CY - London TI - The uncertainty of crop yield projections is reduced by improved temperature response functions. JO - Nat. Plants VL - 3 IS - 8 PB - Nature Publishing Group PY - 2017 SN - 2055-026X ER - TY - JOUR AB - Nature Plants 3, 17102 (2017); published online 17 July 2017; corrected online 27 September 2017. AU - Wang, E.* AU - Martre, P.* AU - Zhao, Z.* AU - Ewert, F.* AU - Maiorano, A.* AU - Rötter, R.P.* AU - Kimball, B.A.* AU - Ottman, M.J.* AU - Wall, G.W.* AU - White, J.W.* AU - Reynolds, M.P.* AU - Alderman, P.D.* AU - Aggarwal, P.K.* AU - Anothai, J.* AU - Basso, B.* AU - Biernath, C.J. AU - Cammarano, D.* AU - Challinor, A.J.* AU - de Sanctis, G.* AU - Doltra, J.* AU - Dumont, B.* AU - Fereres, E.* AU - Garcia-Vila, M.* AU - Gayler, S.* AU - Hoogenboom, G.* AU - Hunt, L.A.* AU - Izaurralde, R.C.* AU - Jabloun, M.* AU - Jones, C.D.* AU - Kersebaum, K.C.* AU - Koehler, A.-K.* AU - Liu, L.* AU - Müller, C.* AU - Kumar, S.N.* AU - Nendel, C.* AU - O'Leary, G.* AU - Olesen, J.E.* AU - Palosuo, T.* AU - Priesack, E. AU - Rezaei, E.E.* AU - Ripoche, D.* AU - Ruane, A.C.* AU - Semenov, M.A.* AU - Shcherbak, I.* AU - Stöckle, C.* AU - Stratonovitch, P.* AU - Streck,T.* AU - Supit, I.* AU - Tao, F.* AU - Thorburn, P.J.* AU - Waha, K.* AU - Wallach, D.* AU - Wang, Z.* AU - Wolf, J.* AU - Zhu, Y.* AU - Asseng, S.* C1 - 52011 C2 - 43644 SP - 1 TI - Author Correction: The uncertainty of crop yield projections is reduced by improved temperature response functions. JO - Nat. Plants PY - 2017 SN - 2055-026X ER - TY - JOUR AB - Finding causal relationships between genotypic and phenotypic variation is a key focus of evolutionary biology, human genetics and plant breeding. To identify genome-wide patterns underlying trait diversity, we assembled a high-quality reference genome of Cardamine hirsuta, a close relative of the model plant Arabidopsis thaliana. We combined comparative genome and transcriptome analyses with the experimental tools available in C. hirsuta to investigate gene function and phenotypic diversification. Our findings highlight the prevalent role of transcription factors and tandem gene duplications in morphological evolution. We identified a specific role for the transcriptional regulators PLETHORA5/7 in shaping leaf diversity and link tandem gene duplication with differential gene expression in the explosive seed pod of C. hirsuta. Our work highlights the value of comparative approaches in genetically tractable species to understand the genetic basis for evolutionary change. AU - Gan, X.* AU - Hay, A.* AU - Kwantes, M.* AU - Haberer, G. AU - Hallab, A.* AU - Ioio, R.D.* AU - Hofhuis, H.* AU - Pieper, B.* AU - Cartolano, M.* AU - Neumann, U.* AU - Nikolov, L.A.* AU - Song, B.* AU - Hajheidari, M.* AU - Briskine, R.* AU - Kougioumoutzi, E.* AU - Vlad, D.* AU - Broholm, S.* AU - Hein, J.* AU - Meksem, K.* AU - Lightfoot, D.* AU - Shimizu, K.K.* AU - Shimizu-Inatsugi, R.* AU - Imprialou, M.* AU - Kudrna, D.* AU - Wing, R.* AU - Sato, S.* AU - Huijser, P.* AU - Filatov, D.* AU - Mayer, K.F.X. AU - Mott, R.F.* AU - Tsiantis, M.* C1 - 49860 C2 - 40997 CY - London TI - The Cardamine hirsuta genome offers insight into the evolution of morphological diversity. JO - Nat. Plants VL - 2 IS - 10 PB - Nature Publishing Group PY - 2016 SN - 2055-026X ER - TY - JOUR AB - Climate projections predict higher precipitation variability with more frequent dry extremes(1). CO2 assimilation of forests decreases during drought, either by stomatal closure(2) or by direct environmental control of sink tissue activities(3). Ultimately, drought effects on forests depend on the ability of forests to recover, but the mechanisms controlling ecosystem resilience are uncertain(4). Here, we have investigated the effects of drought and drought release on the carbon balances in beech trees by combining CO2 flux measurements, metabolomics and (13)CO2 pulse labelling. During drought, net photosynthesis (AN), soil respiration (RS) and the allocation of recent assimilates below ground were reduced. Carbohydrates accumulated in metabolically resting roots but not in leaves, indicating sink control of the tree carbon balance. After drought release, RS recovered faster than AN and CO2 fluxes exceeded those in continuously watered trees for months. This stimulation was related to greater assimilate allocation to and metabolization in the rhizosphere. These findings show that trees prioritize the investment of assimilates below ground, probably to regain root functions after drought. We propose that root restoration plays a key role in ecosystem resilience to drought, in that the increased sink activity controls the recovery of carbon balances. AU - Hagedorn, F.* AU - Joseph, J.* AU - Peter, M.* AU - Luster, J.* AU - Pritsch, K. AU - Geppert, U. AU - Kerner, R.C. AU - Molinier, V.* AU - Egli, S.* AU - Schaub, M.* AU - Liu, J.F.* AU - Li, M.* AU - Sever, K.* AU - Weiler, M.* AU - Siegwolf, R.T.* AU - Gessler, A.* AU - Arend, M.* C1 - 49110 C2 - 41634 CY - London TI - Recovery of trees from drought depends on belowground sink control. JO - Nat. Plants VL - 2 IS - 8 PB - Nature Publishing Group PY - 2016 SN - 2055-026X ER -