TY  - JOUR
AB  - A simple and time‐effective chemoenzymatic process to obtain three regioselective acylated quercetin analogues in a 1:1:1 ratio in one pot is described. This process overcomes the inherent scaffold intricacy and synthetic complexity of quercetin. Cell proliferation experiments in three breast cancers cell lines pinpoint the high potency of the generated compounds.
AU  - Kyriakou, E.
AU  - Kostagianni, A.D.*
AU  - Kellici, T.F.*
AU  - Giannopoulou, E.A.*
AU  - Siatis, K.E.*
AU  - Sayyad, N.*
AU  - Kalofonos, H.P.*
AU  - Mavromoustakos, T.*
AU  - Stamatis, H.*
AU  - Tzakos, A.G.*
C1  - 53586
C2  - 44739
SP  - 5207-5211
TI  - Three regioselectively acylated flavonoid aglycone derivatives in equimolar yield at one blow.
JO  - ChemistrySelect
VL  - 3
IS  - 18
PY  - 2018
SN  - 2365-6549
ER  - 

TY  - JOUR
AB  - Lipases show high stability in lipophilic solvents and catalyze reactions at the water-oil interfaces, which are of great industrial interest. One promising application of lipases is the production of epoxides from alkenes and hydrogen peroxide. So far, little attention has been given to uncover the reaction mechanism for this in detail at the molecular level. Here, we present structural and mutational analysis of a lipase from Penicillium camembertii that indicates a two-stage synergistic mechanism for this reaction. Surprisingly, a mutant devoid of the catalytic serine retains a fraction of activity while histidine from the catalytic triad is absolutely critical to maintain the enzyme activity. Histidine appears to perform a dual-activation role acting both towards hydrogen peroxide and the catalytic serine. These results thus allow a better understanding of enzymatic epoxidation and engineering of more potent, stable and selective enzymes.
AU  - Tang, Q.*
AU  - Popowicz, G.M.
AU  - Wang, X.*
AU  - Liu, J.*
AU  - Pavlidis, I.V.*
AU  - Wang, Y.*
C1  - 52950
C2  - 44255
CY  - Weinheim
SP  - 836-839
TI  - Lipase-driven epoxidation is a two-stage synergistic process.
JO  - ChemistrySelect
VL  - 1
IS  - 4
PB  - Wiley-v C H Verlag Gmbh
PY  - 2016
SN  - 2365-6549
ER  -