TY  - JOUR
AB  - Recent results emphasize the supportive effects of adipose-derived multipotent stem/progenitor cells (ADSPCs) in peripheral nerve recovery. Cultivation under hypoxia is considered to enhance the release of the regenerative potential of ADSPCs. This study aimed to examine whether peripheral nerve regeneration in a rat model of autologous sciatic nerve graft benefits from an additional custom-made fibrin conduit seeded with hypoxic pre-conditioned (2% oxygen for 72 hours) autologous ADSPCs (n = 9). This treatment mode was compared with three others: fibrin conduit seeded with ADSPCs cultivated under normoxic conditions (n = 9); non-cell-carrying conduit (n = 9); and nerve autograft only (n = 9). A 16-week follow-up included functional testing (sciatic functional index and static sciatic index) as well as postmortem muscle mass analyses and morphometric nerve evaluations (histology, g-ratio, axon density, and diameter). At 8 weeks, the hypoxic pre-conditioned group achieved significantly higher sciatic functional index/static sciatic index scores than the other three groups, indicating faster functional regeneration. Furthermore, histologic evaluation showed significantly increased axon outgrowth/branching, axon density, remyelination, and a reduced relative connective tissue area. Hypoxic pre-conditioned ADSPCs seeded in fibrin conduits are a promising adjunct to current nerve autografts. Further studies are needed to understand the underlying cellular mechanism and to investigate a potential application in clinical practice.
AU  - Mayer, J.M.*
AU  - Krug, C.*
AU  - Saller, M.M.*
AU  - Feuchtinger, A.
AU  - Giunta, R.E.*
AU  - Volkmer, E.*
AU  - Holzbach, T.*
C1  - 65974
C2  - 53019
CY  - Wolters Kluwer India Pvt Ltd , A-202, 2nd Flr, Qube, C T S  No 1498a-2 Village Marol, Andheri East, Mumbai, Maharashtra, India
SP  - 652-656
TI  - Hypoxic pre-conditioned adipose-derived stem/progenitor cells embedded in fibrin conduits promote peripheral nerve regeneration in a sciatic nerve graft model.
JO  - Neural Regen. Res.
VL  - 18
IS  - 3
PB  - Wolters Kluwer Medknow Publications
PY  - 2023
SN  - 1876-7958
ER  - 

TY  - JOUR
AB  - Flatworms of the species Schmidtea mediterranea are immortal-adult animals contain a large pool of pluripotent stem cells that continuously differentiate into all adult cell types. Therefore, single-cell transcriptome profiling of adult animals should reveal mature and progenitor cells. By combining perturbation experiments, gene expression analysis, a computational method that predicts future cell states from transcriptional changes, and a lineage reconstruction method, we placed all major cell types onto a single lineage tree that connects all cells to a single stem cell compartment. We characterized gene expression changes during differentiation and discovered cell types important for regeneration. Our results demonstrate the importance of single-cell transcriptome analysis for mapping and reconstructing fundamental processes of developmental and regenerative biology at high resolution.
AU  - Saller, M.M.*
AU  - Huettl, R.E.*
AU  - Mayer, J.M.*
AU  - Feuchtinger, A.
AU  - Krug, C.*
AU  - Holzbach, T.*
AU  - Volkmer, E.*
C1  - 53601
C2  - 44758
CY  - 1200 New York Ave, Nw, Washington, Dc 20005 Usa
SP  - 854-861
TI  - Validation of a novel animal model for sciatic nerve repair with an adipose-derived stem cell loaded fibrin conduit.
JO  - Neural Regen. Res.
VL  - 13
IS  - 5
PB  - Amer Assoc Advancement Science
PY  - 2018
SN  - 1876-7958
ER  -