TY  - JOUR
AB  - Signal propagation in cardiac cell networks can be modulated by heat stimulation. Here, the response of a connected HL-1 cardiomyocyte cell network to the application of confined heat stimuli using Ca 2+ imaging is investigated. Localized temperature gradients are generated by resistive heating via microwire arrays on a chip surface, which serves as a substrate for growing a confluent cell network. It is demonstrated that upon heat stimulation, the velocity of the propagating Ca 2+ wave in the network is locally increased, leading to a deformation of the wavefront. Furthermore, evidence of a change in the signal propagation direction caused by a relocation of the pacemaker cell is shown. This effect might be used in future applications, where heat is employed as an alternative modality for cell stimulation protocols.
AU  - Dang, K.M.*
AU  - Rinklin, P.*
AU  - Afanasenkau, D.*
AU  - Westmeyer, G.G.
AU  - Schürholz, T.*
AU  - Wiegand, S.*
AU  - Wolfrum, B.*
C1  - 55023
C2  - 45995
TI  - Chip-based heat stimulation for modulating signal propagation in HL-1 cell networks.
JO  - Adv. Biosyst.
VL  - 2
IS  - 12
PY  - 2018
SN  - 2366-7478
ER  -