TY  - JOUR
AB  - Nutrient availability influences ribosome biogenesis, requiring dynamic regulation of RNA Pol I activity. In C. elegans , fasting reduces pre-rRNA levels. However, whether this reduction stems from a regulation of RNA Pol I expression remains unclear. Here, we examined how the nutritional status affects the localization and expression levels of RPOA-2 , a core subunit of RNA Pol I, in the intestine. We found that RPOA-2 retains its nucleolar localization regardless of animals being fed, fasted or fed after fasting. Interestingly, fasting reduces RPOA-2 protein amounts which are restored upon feeding. These findings suggest that the availability of RNA Pol I core subunits contributes to the regulation of rDNA transcription in response to nutrients.
AU  - Al-Refaie, N.
AU  - Padovani, F.
AU  - Schmoller, K.M.
AU  - Cabianca, D.S.
C1  - 73429
C2  - 56868
TI  - Localization and expression dynamics of an RNA Pol I core subunit in response to fasting in C. elegans.
JO  - MicroPubl. Biol.
VL  - 2025
PY  - 2025
SN  - 2578-9430
ER  - 

TY  - JOUR
AB  - C-terminal binding proteins (CTBPs) are conserved transcriptional repressors important in cancer and inflammation. Uniquely amongst transcriptional co-regulators, CTBPs possess a functional dehydrogenase domain. Since multiple malignancies display elevated CTBP levels, CTBP inhibitors targeting this dehydrogenase domain have been developed. While the importance of CTBPs dehydrogenase function for transcriptional regulation remains unclear, several studies have relied on CTBP inhibitors. In vitro experiments have confirmed binding of these compounds to CTBP's active site, however evidence for specificity is lacking. To address this, we treated wildtype and Ctbp1 , 2 double knockout J774.1 cells with MTOB or 4-Cl-HIPP and performed RNA-seq. We observed that both inhibitors elicit distinct transcriptional changes indicating non-overlapping modes of action. Moreover, the majority of changes induced by either inhibitor are observed in Ctbp1/2 double knockout cells suggesting off-target effects. We hypothesize that those CTBP dehydrogenase inhibitors lack specificity to CTBPs and emphasise careful revaluation of findings inferred from studies using those inhibitors.
AU  - Stickland, B.A.*
AU  - Greulich, F.*
AU  - Uhlenhaut, N.H.
C1  - 73422
C2  - 57057
TI  - The specificity of CTBP dehydrogenase inhibitors MTOB and 4-Cl-HIPP.
JO  - MicroPubl. Biol.
VL  - 2025
PY  - 2025
SN  - 2578-9430
ER  - 

TY  - JOUR
AB  - Sarm1 is an evolutionary conserved protein that is essential for Wallerian axon degeneration. Sarm1 has emerged as a therapeutic target to treat neuropathies derived from metabolic or chemical stress and physical injury of axons. Yet, the full repertoire of consequences of inhibiting Sarm1 remains unknown. Here we show that loss of Sarm1 in zebrafish does not affect the sensorimotor transformations that underlie rheotaxis. In addition, Sarm1 deficit accelerates the re-growth of regenerating axons. These data indicate that systemic inhibition of Sarm1 is a viable therapeutic option compatible with sustained nervous system function.
AU  - Asgharsharghi, A.
AU  - Tian, W.
AU  - Haehnel-Taguchi, M.*
AU  - López-Schier, H.
C1  - 61558
C2  - 50347
TI  - Sarm1 is dispensable for mechanosensory-motor transformations in zebrafish.
JO  - MicroPubl. Biol.
VL  - 2021
PY  - 2021
SN  - 2578-9430
ER  - 

TY  - JOUR
AU  - Tian, W.
AU  - López-Schier, H.
C1  - 59823
C2  - 49483
TI  - Blocking Wallerian degeneration by loss of Sarm1 does not promote axon resealing in zebrafish.
JO  - MicroPubl. Biol.
VL  - 2020
PY  - 2020
SN  - 2578-9430
ER  -