TY  - JOUR
AB  - Infection of Arabidopsis with avirulent Pseudomonas syringae and exposure to nitrogen dioxide (NO2) both trigger hypersensitive cell death (HCD) that is characterized by the emission of bright blue-green (BG) autofluorescence under UV illumination. The aim of our current work was to identify the BG fluorescent molecules and scrutinize their biosynthesis, localization, and functions during the HCD. Compared with wild-type (WT) plants, the phenylpropanoid-deficient mutant fah1 developed normal HCD except for the absence of BG fluorescence. Ultrahigh resolution metabolomics combined with mass difference network analysis revealed that WT but not fah1 plants rapidly accumulate dehydrodimers of sinapic acid, sinapoylmalate, 5-hydroxyferulic acid, and 5-hydroxyferuloylmalate during the HCD. FAH1-dependent BG fluorescence appeared exclusively within dying cells of the upper epidermis as detected by microscopy. Saponification released dehydrodimers from cell wall polymers of WT but not fah1 plants. Collectively, our data suggest that HCD induction leads to the formation of free BG fluorescent dehydrodimers from monomeric sinapates and 5-hydroxyferulates. The formed dehydrodimers move from upper epidermis cells into the apoplast where they esterify cell wall polymers. Possible functions of phenylpropanoid dehydrodimers are discussed.
AU  - Kanawati, B.
AU  - Bertic, M.
AU  - Moritz, F.
AU  - Habermann, F.*
AU  - Zimmer, I.
AU  - Mackey, D.*
AU  - Schmitt-Kopplin, P.
AU  - Schnitzler, J.-P.
AU  - Durner, J.
AU  - Gaupels, F.
C1  - 68282
C2  - 54703
CY  - The Atrium, Southern Gate, Chichester Po19 8sq, W Sussex, England
TI  - Blue-green fluorescence during hypersensitive cell death arises from phenylpropanoid deydrodimers.
JO  - Plant Direct
VL  - 7
IS  - 9
PB  - John Wiley & Sons Ltd
PY  - 2023
SN  - 2475-4455
ER  - 

TY  - JOUR
AB  - The multinational Arabidopsis research community is highly collaborative and over the past thirty years these activities have been documented by the Multinational Arabidopsis Steering Committee (MASC). Here, we (a) highlight recent research advances made with the reference plant Arabidopsis thaliana; (b) provide summaries from recent reports submitted by MASC subcommittees, projects and resources associated with MASC and from MASC country representatives; and (c) initiate a call for ideas and foci for the “fourth decadal roadmap,” which will advise and coordinate the global activities of the Arabidopsis research community.
AU  - Parry, G.*
AU  - Provart, N.J.*
AU  - Brady, S.M.*
AU  - Uzilday, B.*
AU  - Multinational Arabidopsis Steering Committee (Falter-Braun, P.)
AU  - Multinational Arabidopsis Steering Committee (Mayer, K.F.X.)
C1  - 60991
C2  - 49619
TI  - Current status of the multinational Arabidopsis community.
JO  - Plant Direct
VL  - 4
IS  - 7
PY  - 2020
SN  - 2475-4455
ER  -