TY  - JOUR
AB  - Endophytes inhabit plant tissues, offering various benefits to their hosts.Understanding their roles in sustainable agriculture is a key focus of research. Using16S rRNA gene and fungal specific ITS2 region amplicon sequencing, we investigatedhow 11 different potato cultivars and 3 different growing conditions influence thediversity of microbial endophytes in potato roots. We compared plants grown on 2different soils in greenhouse conditions and plantlets grown in agar medium, i.e., invitro, representing the planting material without the effect of the soil microbiome. Ourstudy reveals that growing conditions significantly influenced the alpha- and beta-diversity of endophytic bacteria. In plants grown in soils, the bacterial endophyticcommunity was mainly represented by the Pseudomonodacae family, whereas for invitro plants, the Paenibacillaceae, a spore-forming bacteria family, was the mainPage 1 of 50representative. The fungal community comprised many possible fungal pathogenssuch as Colletotrichum, Fusarium and Verticillium. For the endophytic fungi both soiltypes and cultivars affected fungal diversity, a stronger effect for cultivars was seen forfungi as compared to bacteria.Overall, our findings indicate that endophytic bacteria exhibit strong recruitmentpotential from soil communities, while the identity of cultivars has also influenced fungaldynamics communities. These findings shed light on the intricate interactions amongpotato cultivars and soil microbiomes, which can affect the design of sustainableagricultural strategies.
AU  - Nepal, J.*
AU  - Guyoneaud, R.*
AU  - Guedea, F.*
AU  - Zhao, T.*
AU  - Vink, S.N.*
AU  - Jia, X.*
AU  - Treder, K.*
AU  - Michałowska, D.*
AU  - Pawłowska, A.*
AU  - Schloter, M.
AU  - Radl, V.
AU  - Salles, J.F.*
AU  - Attard, E.*
C1  - 74741
C2  - 57582
CY  - 3340 Pilot Knob Road, St Paul, Mn 55121 Usa
TI  - Impact of growing conditions and potato cultivars on the diversity of endophytic microbial communities.
JO  - Phytobio. J.
PB  - Amer Phytopathological Soc
PY  - 2025
ER  - 

TY  - JOUR
AB  - Apple replant disease (ARD) occurs when apple is repeatedly planted at 
      the same site, leading to growth reductions and losses in fruit yield 
      and quality. Up to now the etiology is poorly understood, but soil 
      (micro)biota are known to be involved. Since endophytes often colonize 
      plants via the rhizosphere this study aimed at comparing the bacterial 
      endophytic root microbiome in plants growing in ARD affected and 
      unaffected soils from three different sites based on greenhouse biotests
       using a molecular barcoding approach. The initial endophytic microbiome
       of the starting material (in vitro propagated plants of the apple 
      rootstock M26) did not significantly affect the overall richness and 
      diversity of the endophytic community in plants after 8 weeks of growth 
      in the respective soils, but some genera of the initial microbiome 
      managed to establish in apple roots. Proteobacteria were the dominant 
      phylum in all samples. No differences in diversity or number of amplicon
       sequence variants (ASVs) between plants grown in ARD soil and 
      unaffected soil was observed. However, several ASVs of high abundance 
      uniquely found in plants grown in ARD affected soils were Streptomyces. 
      In soil from all three sites these Streptomyces were negatively 
      correlated to plant growth parameters. Future inoculation experiments 
      using selected Streptomyces isolates have to prove if bacteria from this
       genus are opportunists or part of the ARD complex. For the first time, 
      the bacterial endophytic community of apple roots grown in ARD affected 
      soils was characterized which will help to understand the etiology of 
      ARD and develop countermeasures.
AU  - Mahnkopp-Dirks, F.*
AU  - Radl, V.
AU  - Kublik, S.
AU  - Gschwendtner, S.
AU  - Schloter, M.
AU  - Winkelmann, T.*
C1  - 61352
C2  - 49794
CY  - 3340 Pilot Knob Road, St Paul, Mn 55121 Usa
SP  - 177-189
TI  - Molecular barcoding reveals the genus Streptomyces as associated root endophytes of apple (Malus domestica) plants grown in soils affected by apple replant disease.
JO  - Phytobio. J.
VL  - 5
IS  - 2
PB  - Amer Phytopathological Soc
PY  - 2020
ER  -