TY  - JOUR
AB  - INTRODUCTION: Lung cancer remains one of the most prevalent and deadly cancers globally, with high mortality rates largely due to late-stage diagnosis, aggressive progression, and frequent recurrence. Despite advancements in diagnostic techniques and therapeutic interventions, the overall prognosis for lung cancer patients continues to be dismal. METHOD: Emerging research has identified non-coding RNAs (ncRNAs), including microRNAs, long non-coding RNAs, and circular RNAs, as critical regulators of gene expression, significantly influencing cancer biology. These ncRNAs play pivotal roles in various aspects of lung cancer pathogenesis, including tumor initiation, progression, metastasis, and resistance to therapy. RESULTS: We provide a comprehensive analysis of the current understanding of ncRNAs in lung cancer, emphasizing their potential as biomarkers for early diagnosis, prognostication, and the prediction of the therapeutic response. We explore the biological functions of ncRNAs, their involvement in key oncogenic pathways, and the molecular mechanisms by which they modulate gene expression and cellular processes in lung cancer. Furthermore, this review highlights recent advances in ncRNA-based diagnostic tools and therapeutic strategies, such as miRNA mimics and inhibitors, lncRNA-targeted therapies, and circRNA-modulating approaches, offering promising avenues for personalized medicine. CONCLUSION: Finally, we discuss the challenges and future directions in ncRNA research, including the need for large-scale validation studies and the development of efficient delivery systems for ncRNA-based therapies. This review underscores the potential of ncRNAs to revolutionize lung cancer management by providing novel diagnostic and therapeutic options that could improve patient outcomes.
AU  - Suri, C.*
AU  - Swarnkar, S.*
AU  - Bhaskar, L.*
AU  - Verma, H.K.
C1  - 72152
C2  - 56411
TI  - Non-coding RNA as a biomarker in lung cancer.
JO  - Non-coding RNA
VL  - 10
IS  - 5
PY  - 2024
SN  - 2311-553X
ER  - 

TY  - JOUR
AB  - Cold and nutrient‐activated brown adipose tissue (BAT) is capable of increasing systemic energy expenditure via the uncoupled respiration and secretion of endocrine factors, thereby protecting mice against diet‐induced obesity and improving insulin response and glucose tolerance in men. Long non‐coding RNAs (lncRNAs) have recently been identified as fine‐tuning regulators of cellular function. While certain lncRNAs have been functionally characterised in adipose tissue, their overall contribution in the activation of BAT remains elusive. We identified lncRNAs correlating to interscapular brown adipose tissue (iBAT) function in a high fat diet (HFD) and cold stressed mice. We focused on Gm15551, which has an adipose tissue specific expression profile, is highly upregulated during adipogenesis, and downregulated by β‐adrenergic activation in mature adipocytes. Although we performed comprehensive transcriptional and adipocyte physiology profiling in vitro and in vivo, we could not detect an effect of gain or loss of function of Gm15551.
AU  - Engelhard, C.A.*
AU  - Huang, C.*
AU  - Khani, S.
AU  - Kasparek, P.*
AU  - Prochazka, J.*
AU  - Rozman, J.*
AU  - Reguera, D.P.*
AU  - Sedlacek, R.*
AU  - Kornfeld, J.W.*
C1  - 65304
C2  - 52440
TI  - Comprehensive transcriptional profiling and mouse phenotyping reveals dispensable role for adipose tissue selective long noncoding RNA Gm15551.
JO  - Non-coding RNA
VL  - 8
IS  - 3
PY  - 2022
SN  - 2311-553X
ER  - 

TY  - JOUR
AB  - Dictyostelium discoideum is a social amoeba, which on starvation develops from a single-cell state to a multicellular fruiting body. This developmental process is accompanied by massive changes in gene expression, which also affect non-coding RNAs. Here, we investigate how tRNAs as key regulators of the translation process are affected by this transition. To this end, we used LOTTE-seq to sequence the tRNA pool of D. discoideum at different developmental time points and analyzed both tRNA composition and tRNA modification patterns. We developed a workflow for the specific detection of modifications from reverse transcriptase signatures in chemically untreated RNA-seq data at single-nucleotide resolution. It avoids the comparison of treated and untreated RNA-seq data using reverse transcription arrest patterns at nucleotides in the neighborhood of a putative modification site as internal control. We find that nucleotide modification sites in D. discoideum tRNAs largely conform to the modification patterns observed throughout the eukaroytes. However, there are also previously undescribed modification sites. We observe substantial dynamic changes of both expression levels and modification patterns of certain tRNA types during fruiting body development. Beyond the specific application to D. discoideum our results demonstrate that the developmental variability of tRNA expression and modification can be traced efficiently with LOTTE-seq.
AU  - Hoffmann, A.
AU  - Erber, L.*
AU  - Betat, H.*
AU  - Mörl, M.*
AU  - Fallmann, J.*
C1  - 62211
C2  - 50726
TI  - Changes of the tRNA modification pattern during the development of Dictyostelium discoideum.
JO  - Non-coding RNA
VL  - 7
IS  - 2
PY  - 2021
SN  - 2311-553X
ER  -