TY  - JOUR
AB  - The molecular investigation of archived formalin-fixed, paraffin-embedded (FFPE) tissue samples provides the chance to obtain molecular patterns as indicatives for treatment and clinical end points. MALDI mass spectrometry imaging is capable of localizing molecules like proteins and peptides in tissue sections and became a favorite platform for the targeted and non-targeted approaches, especially in clinical investigations for biomarker research. In FFPE tissues the recovery of proteomic information is constrained by fixation-induced cross-links of proteins. The promising new insights obtained from FFPE in combination with the comprehensive patients' data caused much progress in the optimization of MS imaging protocols to investigate FFPE samples. This review presents the past and current research in MALDI MS imaging of FFPE tissues, demonstrating the improvement of analyses, their actual limitations, but also the promising future perspectives for histopathological and tissue-based research.
AU  - Gorzolka, K.
AU  - Walch, A.K.
C1  - 31300
C2  - 34343
SP  - 1365-1376
TI  - MALDI mass spectrometry imaging of formalin-fixed paraffin-embedded tissues in clinical research.
JO  - Histol. Histopathol.
VL  - 29
IS  - 11
PY  - 2014
SN  - 0213-3911
ER  - 

TY  - JOUR
AB  - The mouse is the most commonly used animal for modelling human disease. New approaches for generating genetically manipulated mouse models to represent human disease, as well as target the function of specific genes, has increased the importance of mice in biomedical science. For the correct interpretation of alterations in mouse phenotype the basic morphology of background mouse strains must be known. Despite on-going efforts to create publicly available baseline phenotypic data, the information concerning spontaneous lesions in wild-type mice is incomplete and scattered so far, and further studies are needed. We addressed this problem by screening haematoxylin-eosin stained sections of brain, reproductive organs, urinary bladder, kidney, thyroid, parathyroid, heart, lung, spleen, thymus, lymph nodes, adrenal glands, stomach, intestine, liver, skin and pancreas of six commonly used inbred mouse strains (C57BL6/J, C57BL6/NTac, C3HeB/FeJ, BALB/cByJ, 129P2/OlaHsd and FVB/N) for inherent spontaneous morphological lesions. Interesting spontaneous phenotypes were seen in morphology of the liver, pancreas, adrenal glands, lungs, intestines and heart. In conclusion, care should be taken when choosing the background mouse strain for genetic manipulations, since different mouse strains harbour different inherent lesions that can affect the function of targeted genes, interpretation of results and translation of results to model human disease.
AU  - Serpi, R.
AU  - Klein-Rodewald, T.
AU  - Calzada-Wack, J.
AU  - Neff, F.
AU  - Schuster, T.*
AU  - Gailus-Durner, V.
AU  - Fuchs, H.
AU  - Poutanen, M.*
AU  - Hrabě de Angelis, M.
AU  - Esposito, I.
C1  - 28473
C2  - 33413
SP  - 79-88
TI  - Inbred wild type mouse strains have distinct spontaneous morphological phenotypes.
JO  - Histol. Histopathol.
VL  - 28
IS  - 1
PB  - Hernandez
PY  - 2013
SN  - 0213-3911
ER  - 

TY  - JOUR
AB  - Spectrins are members of the superfamily of F-actin cross linking proteins that are important as scaffolding proteins for protein sorting, cell adhesion, and migration. In addition, spectrins have been implicated in TGF-beta signaling. The aim of the present study was to analyze the expression and localization of beta1-spectrin (SPTBN1) in pancreatic tissues. mRNA levels of SPTBN1 in cultured pancreatic cancer cell lines, as well as in normal pancreatic tissues (n=18), chronic pancreatitis (n=48) and pancreatic cancer tissues (n=66) were analyzed by real time quantitative RT-PCR. Localization of SPTBN1 in pancreatic tissues was determined by immunohistochemistry. SPTBN1 staining was assessed semi-quantitatively in 55 cancer tissues and survival analysis was carried out using the Kaplan-Meier method. Median SPTBN1 mRNA levels were 6.0-fold higher in pancreatic cancer tissues compared to the normal pancreas (p
AU  - Jiang, X.H.*
AU  - Gillen, S.*
AU  - Esposito, I.
AU  - Giese, N.A.*
AU  - Michalski, C.W.*
AU  - Friess, H.*
AU  - Kleeff, J.*
C1  - 2165
C2  - 27715
SP  - 1497-1506
TI  - Reduced expression of the membrane skeleton protein beta1-spectrin (SPTBN1) is associated with worsened prognosis in pancreatic cancer.
JO  - Histol. Histopathol.
VL  - 25
IS  - 12
PB  - Hernandez
PY  - 2010
SN  - 0213-3911
ER  - 

TY  - JOUR
AU  - Werner, M.*
AU  - Müller, J.*
AU  - Walch, A.K.*
AU  - Höfler, H.
C1  - 21249
C2  - 19360
SP  - 553-559
TI  - The molecular pathology of Barrett's esophagus.
JO  - Histol. Histopathol.
VL  - 14
PY  - 1999
SN  - 0213-3911
ER  -