TY  - JOUR
AB  - Conventional cell culture substrates are flat and rigid, locking cells in a permanent and unphysiological geometry. Advanced tissue culture models that emulate the dynamic and 3D environments of organs remain challenging to generate. Here, we establish flexible silicone adhesive films as versatile substrates that enable the on-demand release, transfer, and folding of cultured 2D tissues into 3D geometries. We rolled primary epithelial cultures into tubes, assembled cuboidal structures, and transferred primary endothelial cultures between culture environments for coculturing. Our approach provides an easy-to-implement platform for dynamic geometrical designs in tissue engineering.
AU  - Roth, D.
AU  - Zampa, B.
AU  - Augustin, R.
AU  - Payandehjoo, D.
AU  - Porcella, G.
AU  - Sahin, A.T.
AU  - van der Does, A.M.*
AU  - Nawroth, J.
C1  - 75764
C2  - 57970
CY  - 1155 16th St, Nw, Washington, Dc 20036 Usa
SP  - 6506-6514
TI  - Release, transfer, fold: Using a silicone adhesive for on-demand 3D tissue engineering.
JO  - ACS Biomater. Sci. Eng.
VL  - 11
IS  - 11
PB  - Amer Chemical Soc
PY  - 2025
SN  - 2373-9878
ER  - 

TY  - JOUR
AB  - Superparamagnetic iron oxide nanoparticles (SPIONs) have gained increasing interest in nanomedicine, but most of those that have entered the clinical trials have been withdrawn due to toxicity concerns. Therefore, there is an urgent need to design low-risk and biocompatible SPION formulations. In this work, we present an original safe-by-design nanoplatform made of silica nanoparticles loaded with SPIONs and decorated with polydopamine (SPIONs@SiO2-PDA) and the study of its biocompatibility performance by an ad hoc thorough in vitro to in vivo nanotoxicological methodology. The results indicate that the SPIONs@SiO2-PDA have excellent colloidal stability in serum-supplemented culture media, even after long-term (24 h) exposure, showing no cytotoxic or genotoxic effects in vitro and ex vivo. Physiological responses, evaluated in vivo using Caenorhabditis elegans as the animal model, showed no impact on fertility and embryonic viability, induction of an oxidative stress response, and a mild impact on animal locomotion. These tests indicate that the synergistic combination of the silica matrix and PDA coating we developed effectively protects the SPIONs, providing enhanced colloidal stability and excellent biocompatibility.
AU  - Romano, M.
AU  - González Gómez, M.A.*
AU  - Santonicola, P.*
AU  - Aloi, N.*
AU  - Offer, S.
AU  - Pantzke, J.
AU  - Raccosta, S.*
AU  - Longo, V.*
AU  - Surpi, A.*
AU  - Alacqua, S.
AU  - Zampi, G.*
AU  - Dediu, V.A.*
AU  - Michalke, B.
AU  - Zimmermann, R.
AU  - Manno, M.*
AU  - Piñeiro, Y.*
AU  - Colombo, P.*
AU  - Di Schiavi, E.*
AU  - Rivas, J.*
AU  - Bergese, P.*
AU  - Di Bucchianico, S.
C1  - 67001
C2  - 53403
CY  - 1155 16th St, Nw, Washington, Dc 20036 Usa
SP  - 303-317
TI  - Synthesis and characterization of a biocompatible nanoplatform based on silica-embedded SPIONs functionalized with polydopamine.
JO  - ACS Biomater. Sci. Eng.
VL  - 9
IS  - 1
PB  - Amer Chemical Soc
PY  - 2023
SN  - 2373-9878
ER  -