TY - JOUR AB - Autofucusing is the fundamental step when it comes to image acquistion and analysis with automated microscopy devices. Despite all efforts that have been put into developing a reliable autofocus system, recent methods still lack robustness towards fifferent microscope modes and distracting artefacts. This paper presents a novel automated focusing approach that is generally applicable to different microscope modes (bright-field, phase contrast, Differential Interference Contrast (DIC) and fluorescence microscopy). The main innovation consists in a Content-based focus search that makes use of a priori knowledge about the observed objects by employing local object features and Boosted Learning. Hence, this method turns away from common autofocus approaches that apply solely whole image frequency measurements to obrein the focus plane. Thus, it is possible to exclude artefacts from being brought into focus calculatin as well as locating the in-focus layer of specific microscopic objects. AU - Hamm, P. AU - Schulz, J.* AU - Englmeier, K.-H. C1 - 622 C2 - 28020 SP - 173-180 TI - Content-based autofocusing in automated microscopy. JO - Image Anal. Stereol. VL - 29 PY - 2010 SN - 1580-3139 ER - TY - JOUR AU - Rodenacker, K. AU - Hausner, M.* AU - Kuhn, M.* AU - Wuertz, St.* AU - Purkayastha, S.* C1 - 9526 C2 - 21872 SP - 556-560 TI - Depth intensity correction of biofilm volume data from confocal laser scanning microscopes. JO - Image Anal. Stereol. VL - 20 PY - 2001 SN - 1580-3139 ER - TY - JOUR AU - Jütting, U. AU - Gais, P. AU - Rodenacker, K. AU - Böhm, J.* AU - Höfler, H. C1 - 21348 C2 - 19464 SP - 39-43 TI - MIB-1, AgNOR and DNA distribution parameters and their prognostic value in neuroendocrine tumours of the lung. JO - Image Anal. Stereol. VL - 19 PY - 2000 SN - 1580-3139 ER - TY - JOUR AU - Rodenacker, K. AU - Brühl, A.* AU - Hausner, M.* AU - Kuhn, M.* AU - Liebscher, V. AU - Wagner, M.* AU - Winkler, G. AU - Wuertz, St.* C1 - 21347 C2 - 19463 SP - 151-156 TI - Quantification of biofilms in multi-special digital volumes from confocal laser-scanning microscopes. JO - Image Anal. Stereol. VL - 19 PY - 2000 SN - 1580-3139 ER - TY - JOUR AU - Rodenacker, K. AU - Hahn, K.R. AU - Winkler, G. AU - Auer, D.P.* C1 - 21744 C2 - 19922 SP - 189-194 TI - Spatio-temporal data analysis with non-linear filters : Brain mapping with fMRI data. JO - Image Anal. Stereol. VL - 19 PY - 2000 SN - 1580-3139 ER -