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Fuchs, Y.F.* ; Sharma, V.* ; Eugster, A.* ; Kraus, G.* ; Morgenstern, R.* ; Dahl, A.* ; Reinhardt, S.* ; Petzold, A.* ; Lindner, A.* ; Löbel, D.* ; Bonifacio, E.

Gene expression-based identification of antigen-responsive CD8+ T cells on a single-cell level.

Front. Immunol. 10:2568 (2019)
Verlagsversion DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
CD8(+) T cells are important effectors of adaptive immunity against pathogens, tumors, and self antigens. Here, we asked how human cognate antigen-responsive CD8(+) T cells and their receptors could be identified in unselected single-cell gene expression data. Single-cell RNA sequencing and qPCR of dye-labeled antigen-specific cells identified large gene sets that were congruently up- or downregulated in virus-responsive CD8(+) T cells under different antigen presentation conditions. Combined expression of TNFRSF9, XCL1, XCL2, and CRTAM was the most distinct marker of virus-responsive cells on a single-cell level. Using transcriptomic data, we developed a machine learning-based classifier that provides sensitive and specific detection of virus-responsive CD8(+) T cells from unselected populations. Gene response profiles of CD8(+) T cells specific for the autoantigen islet-specific glucose-6-phosphatase catalytic subunit-related protein differed markedly from virus-specific cells. These findings provide single-cell gene expression parameters for comprehensive identification of rare antigen-responsive cells and T cell receptors.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Cd8(+) T Cells ; Single-cell ; Antigen-responsive ; Gene-expression Analysis ; Ctl (cytotoxic T Lymphocyte) ; Influenza Matrix Protein ; Cmv Pp65 ; T Cell Receptor (tcr); Parallel Detection; Immune-responses; Ifn-gamma; Requires; Memory; Infection; Expansion; Egr2
Sprache englisch
Veröffentlichungsjahr 2019
HGF-Berichtsjahr 2019
ISSN (print) / ISBN 1664-3224
e-ISSN 1664-3224
Zeitschrift Frontiers in Immunology
Quellenangaben Band: 10, Heft: , Seiten: , Artikelnummer: 2568 Supplement: ,
Verlag Frontiers
Verlagsort Avenue Du Tribunal Federal 34, Lausanne, Ch-1015, Switzerland
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Diabetes and Obesity (IDO)
POF Topic(s) 90000 - German Center for Diabetes Research
Forschungsfeld(er) Helmholtz Diabetes Center
PSP-Element(e) G-501900-229
DOI 10.3389/fimmu.2019.02568
WOS ID WOS:000500777200001
Scopus ID 85075786909
PubMed ID 31781096
Erfassungsdatum 2019-12-02
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