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Open Access Green as soon as Postprint is submitted to ZB.
Impact of Hey2 and COUP-TFII on genes involved in arteriovenous differentiation in primary human arterial and venous endothelial cells.
Basic Res. Cardiol. 108:362 (2013)
Arteries and veins show marked differences in their anatomy, physiology and genetic expression pattern. In this study, we analyzed impact of overexpression or downregulation of arterial marker gene Hey2 and venous marker gene COUP-TFII in human venous and arterial endothelial cells on genes involved in arteriovenous differentiation. Lentiviral overexpression of venous marker gene COUP-TFII in arterial endothelial cells led to downregulation of NICD4, arterial marker gene Hey2 and EphrinB2. Downregulation of Hey2 could be mediated by direct binding of COUP-TFII to Hey2 promoter as shown by ChIP, EMSA and promoter analysis. Downregulation of Hey2 by shRNA causes downregulation of EphrinB2 expression. Overexpression of arterial marker Hey2 in venous endothelial cells did not change expression pattern of COUP-TFII. Downregulation of venous marker gene COUP-TFII in venous endothelial cells resulted in upregulation of VEGF-A, Dll4 and EphrinB2 expression. Our data support an important role of Hey2 and COUP-TFII in arteriovenous differentiation of human endothelial cells.
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Publication type
Article: Journal article
Document type
Scientific Article
ISSN (print) / ISBN
0300-8428
e-ISSN
1435-1803
Journal
Basic Research in Cardiology
Quellenangaben
Volume: 108,
Issue: 4,
Article Number: 362
Publisher
Springer
Reviewing status
Peer reviewed
Institute(s)
Institute of Pancreatic Islet Research (IPI)