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Speier, S.* ; Nyqvist, D.* ; Cabrera, O.* ; Yu, J.* ; Molano, R.D.* ; Pileggi, A.* ; Moede, T.* ; Köhler, M.* ; Wilbertz, J.* ; Leibiger, B.* ; Ricordi, C.* ; Leibiger, I.B.* ; Caicedo, A.* ; Berggren, P.O.*

Noninvasive in vivo imaging of pancreatic islet cell biology.

J. Nat. Med. 14, 574-578 (2008)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Advanced imaging techniques have become a valuable tool in the study of complex biological processes at the cellular level in biomedical research. Here, we introduce a new technical platform for noninvasive in vivo fluorescence imaging of pancreatic islets using the anterior chamber of the eye as a natural body window. Islets transplanted into the mouse eye engrafted on the iris, became vascularized, retained cellular composition, responded to stimulation and reverted diabetes. Laser-scanning microscopy allowed repetitive in vivo imaging of islet vascularization, beta cell function and death at cellular resolution. Our results thus establish the basis for noninvasive in vivo investigations of complex cellular processes, like beta cell stimulus-response coupling, which can be performed longitudinally under both physiological and pathological conditions.
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Publication type Article: Journal article
Document type Scientific Article
ISSN (print) / ISBN 1340-3443
e-ISSN 1861-0293
Journal Journal of natural medicines
Quellenangaben Volume: 14, Issue: 5, Pages: 574-578 Article Number: , Supplement: ,
Publisher Springer
Publishing Place Tokyo [u.a.]
Reviewing status Peer reviewed
Institute(s) Abteilung Biophysikalische Strahlenforschung