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Breidert, M.* ; Miehlke, S.* ; Glasow, A.* ; Orban, Z.* ; Stolte, M.* ; Ehninger, G.* ; Bayerdörffer, E.* ; Nettesheim, O.* ; Halm, U.* ; Haidan, A.* ; Bornstein, S.R.*

Leptin and its receptor in normal human gastric mucosa and in Helicobacter pylori-associated gastritis.

Scand. J. Gastroenterol. 34, 954-961 (1999)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
BACKGROUND: Leptin, a newly discovered weight-reducing hormone, is mainly produced in fat cells. Recently, this hormone has been reported to be produced in rat gastric mucosa cells. In the present study we analyzed the localization and expression of leptin and its receptors in normal human gastric mucosa and in patients with Helicobacter pylori-associated gastritis. METHODS: Plasma leptin levels and gastric mucosa leptin content were determined in 39 patients with dyspepsia. Cellular localization of leptin and of the signaling receptor (Ob-RL) were assessed by immunohistochemistry. Reverse transcriptase polymerase chain reaction (RT-PCR) for leptin receptor isoforms was performed on gastric epithelial cells isolated by laser-capture-microdissection. RESULTS: Leptin content of the corpus gastric mucosa in H. pylori-positive patients was significantly increased (4.6+/-1.2. n = 15) as compared with the H. pylori-negative group (27.5+/-0.5 pg/mg, n = 24, P = 0.006). The presence of leptin immunoreactivity was shown in the lower half of corpus epithelial glands. By RT-PCR no leptin mRNA was detectable in human gastric tissue. In contrast, expression of both Ob-R(L) and the leptin receptor isoforms could be detected in gastric epithelial cells. Leptin receptor protein was detected throughout the mucosa. CONCLUSIONS: Leptin itself is stored and secreted but not produced in human gastric mucosa. The functional receptor and all isoforms are present in human gastric mucosa. H. pylori-associated gastritis leads to significant increases in local leptin concentration in the gastric corpus.
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Publication type Article: Journal article
Document type Scientific Article
ISSN (print) / ISBN 0036-5521
e-ISSN 1502-7708
Journal Scandinavian Journal of Gastroenterology
Quellenangaben Volume: 34, Issue: 10, Pages: 954-961 Article Number: , Supplement: ,
Publisher Informa Healthcare
Reviewing status Peer reviewed
Institute(s) Institute of Pancreatic Islet Research (IPI)