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Willeke, F.* ; Mechtersheimer, G.* ; Schwarzbach, M.* ; Weitz, J.* ; Zimmer, D.* ; Lehnert, T.* ; Herfarth, C.* ; von Knebel Doeberitz, M.* ; Ridder, R.*

Detection of SYT-SSX1/2 fusion transcripts by reverse transcriptase-polymerase chain reaction (RT-PCR) is a valuable diagnostic tool in synovial sarcoma.

Eur. J. Cancer 34, 2087-2093 (1998)
PMC
Open Access Green as soon as Postprint is submitted to ZB.
Cytogenetically, most synovial sarcomas are characterised by a specific chromosomal translocation [(X;18) (p11.2;q11.2)], which results in the generation of fusion transcripts comprising SYT (18q11) and either SSX1 or SSX2 (Xp11) sequences. By using a sensitive reverse transcriptase-polymerase chain reaction (RT-PCR) protocol, specific SYT-SSX1/2 fusion transcripts were detected in 10 histopathologically confirmed synovial sarcomas. Control tumours with morphological spindle cell patterns mimicking monophasic synovial sarcoma tested negative (18/19) in the RT-PCR protocol, with the exception of one spindle cell sarcoma originally classified as a fibrosarcoma. Furthermore, the established RT-PCR protocol was used to evaluate the feasibility of SYT-SSX1/2 fusion transcript detection for minimal residual disease analysis. Analyses of surgical margins revealed a fusion transcript in two of four operations for synovial sarcoma analysed, one of which was diagnosed with tumour free margins by conventional histopathology. These data suggest that the RT-PCR amplification of SYT-SSX1/2 fusion transcripts is a valuable tool in the differentiation of synovial sarcomas, especially in cases of equivocal morphology. Additionally, the RT-PCR approach may be used for the detection of residual tumour cells in synovial sarcoma patients.
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Publication type Article: Journal article
Document type Scientific Article
ISSN (print) / ISBN 0959-8049
e-ISSN 1879-0852
Journal European Journal of Cancer
Quellenangaben Volume: 34, Issue: 13, Pages: 2087-2093 Article Number: , Supplement: ,
Publisher Elsevier
Reviewing status Peer reviewed
Institute(s) Institute of Pancreatic Islet Research (IPI)