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Kim, J.* ; Xie, S.* ; Valenzuela, L.F.* ; Xiao, J.* ; Lanz, M.C.* ; Gao, X.* ; Swaffer, M.* ; Mitchell, C.E.* ; Rubin, S.M.* ; Schmoller, K.M. ; Skotheim, J.M.*

A Fkh1/2 binding site array in the WHI5 promoter drives sub-scaling transcription.

Cell Rep. 45:117304 (2026)
Verlagsversion Forschungsdaten DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Cells maintain size homeostasis by coupling growth to division. In budding yeast, newborn cells contain similar amounts of the G1/S inhibitor Whi5, which is diluted as cells grow in G1 to promote cell cycle entry. Similar Whi5 amounts at birth arise from size-independent (sub-scaling) WHI5 mRNA production during S/G2/M and equal partitioning of Whi5 at division. Although chromatin association explains equal partitioning at division, the basis of sub-scaling transcription remained unclear. By systematically mutating the WHI5 promoter, we identify a core region from -126 to -75 bp upstream of the start codon that is responsible for sub-scaling. This sequence contains a repeating array of binding sites for the Fkh1/2 transcription factor. Mutating these sites, deleting FKH1 or FKH2, or disrupting Fkh1/2 dimerization weakens WHI5 sub-scaling. Together with structural predictions and a mathematical model of cooperative Fkh binding, our results suggest that sub-scaling WHI5 transcription is regulated by a Fkh1/2 heteropolymer that binds an array of sites in its core promoter.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Cp: Cell Biology ; Cp: Genomics ; G1/s Transition ; Whi5 Dilution ; Cell Cycle Control ; Cell Size Control ; Cooperative Dna Binding ; Forkhead Transcription Factors ; Size Scaling ; Sub-scaling Transcription; Cell-size Regulation; Budding-yeast; Gene-expression; Copy Number; Whi5; Hcm1
ISSN (print) / ISBN 2211-1247
e-ISSN 2211-1247
Zeitschrift Cell Reports
Quellenangaben Band: 45, Heft: 5, Seiten: , Artikelnummer: 117304 Supplement: ,
Verlag Elsevier
Verlagsort 50 Hampshire St, Floor 5, Cambridge, Ma 02139 Usa
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Functional Epigenetics (IFE)
Förderungen NIH