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qPCR zur quantitativen Validierung von Metagenomdaten.
BioSpektrum 22, 265-269 (2016)
Microorganisms are essential to maintain ecosystem functions and health as they catalyze most keystone processes. Formerly the quantification of key functions was performed by broad ranged primers, which were mainly based on genomes of cultivated microbes. To take advantage of the enormous development of next generation sequencing technologies, we propose to use metagenomic data for the design of new primer systems. Here we describe a two-phasic approach for a targeted and site specific primer design for qPCR.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
ISSN (print) / ISBN
0947-0867
e-ISSN
1868-6249
Zeitschrift
BioSpektrum
Quellenangaben
Band: 22,
Heft: 3,
Seiten: 265-269
Verlag
Springer
Begutachtungsstatus
Peer reviewed