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Sharma, S. ; Radl, V. ; Hai, B. ; Kloos, K. ; Mrkonjic, M. ; Engel, M. ; Schauss, K. ; Schloter, M.

Quantification of functional genes from procaryotes in soil by PCR.

J. Microbiol. Methods 68, 445-452 (2007)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Controlling turnover processes and fluxes in soils and other environments requires information about the gene pool and possibilities for its in situ induction. Therefore in the recent years there has been a growing interest in genes and transcripts coding for metabolic enzymes. Besides questions addressing redundancy and diversity, more and more attention is given on the abundance of specific DNA and mRNA in the different habitats. This review will describe several PCR techniques that are suitable for quantification of functional genes and transcripts such as MPN-PCR, competitive PCR and real-time PCR. The advantages and disadvantages of the mentioned methods are discussed. In addition, the problems of quantitative extraction of nucleic acid and substances that inhibit polymerase are described. Finally, some examples from recent papers are given to demonstrate the applicability and usefulness of the different approaches.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter quantitative PCR; real-time PCR; MPN-PCR; competitive PCR; microbial activity
ISSN (print) / ISBN 0167-7012
e-ISSN 1872-8359
Zeitschrift Journal of Microbiological Methods
Quellenangaben Band: 68, Heft: 3, Seiten: 445-452 Artikelnummer: , Supplement: ,
Verlag Elsevier
Verlagsort Amsterdam
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Soil Ecology (IBOE)