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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Analysis of N-acyl-l-homoserine lactones produced by Burkholderia cepacia with partial filling micellar electrokinetic chromatography - electrospray ionization-ion trap mass spectrometry.
Electrophoresis 24, 3067-3074 (2003)
A method for the analysis of N-acyl-L-homoserine lactones (AHLs) with micellar electrokinetic chromatography coupled to electrospray ionization-ion trap mass spectrometry, combining the flexibility of capillary electrophoresis with the unmatched structural information provided by mass spectrometry is presented. Different surfactants were evaluated, with sodium dodecyl sulfate (SDS) yielding the best results considering sensitivity and flexibility. We examined the interaction of AHLs with the SDS micelles at different analysis conditions and applied the optimized method to the analysis of a real bacterial sample. Two AHLs from Burkholderia cepacia colonizing the rhizosphere of traditional Indian rice cultivars could be unambiguously determined in an ethyl acetate extract with high resolution flexibility.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Burkholderia cepacia; Electrospray ionization-mass specrometry; Micellar electrokinetic chromatography; N-Acyl-l-homoserine; Quorum sensing
ISSN (print) / ISBN
0173-0835
e-ISSN
1522-2683
Zeitschrift
Electrophoresis
Quellenangaben
Band: 24,
Heft: 17,
Seiten: 3067-3074
Verlag
Wiley
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute of Ecological Chemistry (IOEC)
Institute of Soil Ecology (IBOE)
Institute of Soil Ecology (IBOE)