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Eichholtz-Wirth, H. ; Sagan, D.

IkB/NF-kB mediated cisplatin resistance in HeLa cells after low-dose gamma-irradiation is associated with altered SODD expression.

Apoptosis 5, 255-263 (2000)
DOI
Open Access Gold möglich sobald Verlagsversion bei der ZB eingereicht worden ist.
Fractionated γ-irradiation (15 × 2 Gy in 3 weeks) induces a cellular resistance in HeLa cells against cisplatin exposure but not against irradiation. The mechanisms underlying this cellular resistance are associated with major changes in the TNFR1-dependent transduction pathway. The resistant HeLa/B cells exhibit increased levels of NF-κB with temporally independent regulation of the subunits NF-κB50 and NF-κB65. Blocking IκB degradation by the proteasome inhibitor PSI, which abolishes the release of the active NF-κB protein, induces cell death much more effectively in the parental than in the resistant HeLa/B cells. The translocation of NF-κB does not seem to be affected in a similar manner since masking of the translocation sequence by NF-κB SN50 enhances cisplatin toxicity to the same degree in both cell lines and overcomes drug resistance. Changes in upstream signaling are suggested by increased sensitivity of the parental HeLa cells to cisplatin in the presence of neutralizing anti-TNFR1. In HeLa/B cells, reduced expression of the 50 kDa silencer of death domain, SODD, is accompanied by constitutive overexpression of a 40–42 kDa SODD-like protein. A possible involvement of SODD in cisplatin resistance is discussed, which may shift the balance between life and death in the TNF receptor pathway to increased NF-κB activation.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter cisplatin resistance; irradiation; NF-kB; SODD
ISSN (print) / ISBN 1360-8185
e-ISSN 1573-675X
Zeitschrift Apoptosis
Quellenangaben Band: 5, Heft: 3, Seiten: 255-263 Artikelnummer: , Supplement: ,
Verlag Springer
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Radiation Biology (ISB)