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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Molecular live cell bioimaging in stem cell research.
Ann. NY Acad. Sci. 1266, 18-27 (2012)
Functional heterogeneity within stem and progenitor cells has been shown to influence cell fate decisions. Similarly, intracellular signaling activated by external stimuli is highly heterogeneous and its spatiotemporal activity is linked to future cell behavior. To quantify these heterogeneous states and link them to future cell fates, it is important to observe cell populations continuously with single cell resolution. Live cell imaging in combination with fluorescent biosensors for signaling activity serves as a powerful tool to study cellular and molecular heterogeneity and the long-term biological effects of signaling. Here, we describe these methodologies, their advantages over classical approaches, and we illustrate how they could be applied to improve our understanding of the importance of heterogeneous cellular and molecular responses to external signaling cues.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
biosensors; cytokines; M-CSF; signaling; lineage choice; stem cell; imaging; time lapse; Protein-Kinase-C; Stimulating Factor-I; Hematopoietic Progenitor Cells; Infrared Fluorescent Proteins; Receptor Tyrosine Kinase; M-Csf Receptor; Self-Renewal; Lineage Commitment; Living Cells; Scaffolding Protein
ISSN (print) / ISBN
0077-8923
e-ISSN
1749-6632
Zeitschrift
Annals of the New York Academy of Sciences
Quellenangaben
Band: 1266,
Heft: 1,
Seiten: 18-27
Verlag
New York Academy of Sciences
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed
Institut(e)
Research Unit Stem Cell Dynamics (SCD)