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Baldini, F.* ; Bolzoni, L.* ; Giannetti, A.* ; Keß, M. ; Krämer, P. ; Kremmer, E. ; Porro, G.* ; Senesi, F.* ; Trono, C.*

A new procalcitonin optical immunosensor for POCT applications.

Anal. Bioanal. Chem. 393, 1183-1190 (2009)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
A new immunosensor for the determination of procalcitonin was developed. A sandwich assay format was implemented on a polymethylmetacrylate optical biochip, opportunely shaped in order to obtain several flow channels and potentially suitable for point of care testing applications. The sandwich format makes use of two new rat monoclonal antibodies. The capture antibody was covalently immobilised on the surface of the plastic chip, and the detection antibody was labelled with DY647 dye. Different combinations of capture and detection antibodies were investigated, and particular attention was devoted in order to avoid the nonspecific adsorption. A limit of detection of 0.088 mg L-1 was achieved within the working range of 0.28-50 mg L-1 in buffer samples. The assay was also implemented in human serum, and 0.2 and 0.7-25 mg L-1 were the attained limit of detection and working range, respectively.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Optical biochip; Immunosensor; Procalcitonin; PMMA; Fluorescence anisotropy; POCT; serum procalcitonin; infection; bacterial; sepsis
ISSN (print) / ISBN 1618-2642
e-ISSN 1618-2650
Zeitschrift Analytical and Bioanalytical Chemistry
Quellenangaben Band: 393, Heft: 4, Seiten: 1183-1190 Artikelnummer: , Supplement: ,
Verlag Springer
Verlagsort Heidelberg
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Ecological Chemistry (IOEC)
Institute of Molecular Immunology (IMI)
Research Unit Microbe-Plant Interactions (AMP)