PuSH - Publikationsserver des Helmholtz Zentrums München: Combined immunophenotyping and fish ('Fish-Ipa') with chromosome-specific DNA-probes allows the quantification and differentation of ex vivo generated dendritic cells (DC), leukemia-derived DC and clonal leukemic cells in patients with AML.

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Kremser, A.* ; Kufner, S.* ; Konhaeuser, E. ; Kroell, T.* ; Hausmann, A.* ; Tischer, J.* ; Kolb, H.-J.* ; Zitzelsberger, H. ; Schmetzer, H.M.

Combined immunophenotyping and fish ('Fish-Ipa') with chromosome-specific DNA-probes allows the quantification and differentation of ex vivo generated dendritic cells (DC), leukemia-derived DC and clonal leukemic cells in patients with AML.

Leuk. Lymphoma 54, 1297-1308 (2013)

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Abstract Antileukemic-T-cell-responses, induced by leukemia-derived-dendritic-cells (DC(leu)) are variable, due to varying DC/DC(leu)-composition/quality. We studied DC/DC(leu)-composition/quality after blast-culture in four DC-media by flowcytometry (FC) and combined FISH/immunophenotyping-analysis(FISH-IPA). Both methods showed, that DC-methods produce variable proportions of DC-subtypes. 1)FISH-IPA is an elaborate method to study clonal aberrations in blast/DC-cells on slides, however without preselection of distinct cell-populations for FISH-analysis. 2)FISH-IPA-data proved previous FC-data: not every clonal/blast cell is converted to DC(leu) (resulting in various proportions of DC(leu)) and not every detectable DC is of clonal/leukemic origin. 3)Preselection of the best of four DC-methods for 'best' DC/DC(leu)-generation is necessary. 4)DC(leu)-proportions correlate with the antileukemic functionality of DC/DC(leu)-stimulated T-cells, thereby proving the necessity of studying quality of DC/DC(leu) after culture. 5)FC is the superior method to quantify DC/DC(leu), since a blast-phenotype is available in every given patient, even with low/no proportions of clonal aberrations, and can easily be used to study cellular compositions after DC-culture.

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