PuSH - Publikationsserver des Helmholtz Zentrums München: C/EBPβ expression in ALK-positive anaplastic large cell lymphomas (ALCL) is required for cell proliferation and is induced by the STAT3 signaling pathway.

Informationen

Hinweise zu Qualitätskriterien von Zeitschriften

Open-Access-Richtlinie der Helmholtz-Gemeinschaft 2016

CC Licencences

Metriken für Publikationen

Navigation

Startseite

English

Recherche

Erweiterte Suche

Durchblättern nach ...

... HMGU-Autoren/Konsortien

... Organisationsstruktur

... Zeitschriften

... Publikationstypen

... Forschungsdaten

... Arbeitsgruppen

... Erscheinungsjahr

Publikationen im Überblick

Statistik

HGF Fortschrittsbericht

OA Publikationen

Eintragen

Neue Publikation eintragen

Neue Publikation holen aus...

...EVA

Fehlende Publikation melden

Highlights

Suche

Hilfe & Kontakt

Ansprechpartner

Hilfe

Datenschutz

Helmholtz Open Science

Bibliometrische Indikatoren

SHERPA/RoMEO

DOAJ

Export:

Text

Endnote (RIS) BIB

BibTeX

Anastasov, N. ; Bonzheim, I.* ; Rudelius, M.* ; Klier, M.* ; Dau, T. ; Angermeier, D. ; Duyster, J.* ; Pittaluga, S.* ; Fend, F.* ; Raffeld, M.* ; Quintanilla-Martinez, L.

C/EBPβ expression in ALK-positive anaplastic large cell lymphomas (ALCL) is required for cell proliferation and is induced by the STAT3 signaling pathway.

Haematologica 95, 760-767 (2010)

Verlagsversion Volltext

DOI

PMC

	Open Access Gold

Abstract
Metriken
Zusatzinfos

Background: ALK+ALCL is characterized by the t(2;5) chromosomal translocation, resulting in the expression of the fusion protein NPM-ALK. Recently, we reported the abnormal expression of the transcription factor C/EBPβ in ALK+ALCL, and demonstrated its dependence on NPM-ALK kinase activity. Design and Methods: In this study, the role of C/EBPβ in proliferation and survival of ALK+ALCL was investigated, as well as, the mechanism of its expression and activity. Highly effective shRNA sequences and/or pharmacological inhibitors were used to abrogate C/EBPβ, STAT3, AKT, ERK1/2 and mTOR expression or activity.. Results: Interference with C/EBPβ expression resulted in a dramatic decrease in cell proliferation in ALK+ALCLs, with a mild induction of apoptosis after 6 days. Downregulation of STAT3 resulted in a marked decrease in C/EBPβ mRNA and protein levels with impairment in cell proliferation and viability, underscoring the important role of these two proteins in ALK-mediated oncogenesis. Additionally, we demonstrated that reduction of ERK1/2 activity led to C/EBPβ Thr235 dephosphorylation and moderate growth retardation. The AKT/mTOR signaling pathway did not have any influence on C/EBPβ expression or C/EBPβ phosphorylation. Conclusions: These findings reveal the convergence of STAT3 and ERK1/2 signaling pathways activated by NPM-ALK, in mediating the regulation of C/EBPβ expression, a transcription factor central to NPM-ALK transformation.

Impact Factor

Scopus SNIP

Web of Science
Times Cited

Scopus
Cited By

Altmetric

5.978

1.280